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Ma hydrochloride

Manufactured by Merck Group
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(+)MA hydrochloride is a chemical compound used in research laboratories. It serves as a key intermediate in the synthesis of various pharmaceutical and biological compounds. The product specification and technical details are available upon request.

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Lab products found in correlation

Sodium chloride (nacl), by Merck Group (2 mentions) Quinine hemisulfate, by Merck Group (1 mentions) Saccharin sodium salt, by Merck Group (1 mentions) Nicotine hydrogen tartrate, by Merck Group (1 mentions)

9 protocols using ma hydrochloride

1

Chronic Methamphetamine Administration Protocol

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MA hydrochloride was purchased from Sigma-Aldrich (St. Louis, MO). MA was prepared by dissolving MA hydrochloride in saline solution to produce concentrations of 4 mg/kg and 8 mg/kg. After a one week acclimation period to the animal facility, rats began MA treatment. Rats were randomly assigned to receive vehicle (saline), or low dose (LD) 4mg/kg MA, or high dose (HD) 8 mg/kg MA, via daily intraperitoneal injections for four months (1 ml/kg body weight).
Thanos P.K., Kim R., Delis F., Ananth M., Chachati G., Rocco M.J., Masad I., Muniz J.A., Grant S.C., Gold M.S., Cadet J.L, & Volkow N.D. (2016). Chronic Methamphetamine Effects on Brain Structure and Function in Rats. PLoS ONE, 11(6), e0155457.
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2

Genotyping Taar1 Gene in Mice

Cited 1 time
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(+)MA hydrochloride was purchased from Sigma Aldrich (St. Louis, MO, USA) and dissolved in tap water for drinking studies or in sterile 0.9% saline (Baxter Healthcare Corp., Deerfield, IL, USA) for IP injection. Saccharin sodium salt (SACC) and quinine hemisulfate (QUIN) were obtained from Sigma Aldrich and dissolved in tap water for tastant studies. Genomic DNA was extracted from ear punch samples using QuickExtract DNA extraction solution (Qiagen, Valecia, CA, USA) and Taar1 was amplified (forward 5’-ctttctgctgggctgtctga-3’, reverse 5’-caacagcgctcaacagttctc-3’) and genotype determined utilizing a rtPCR assay, based on standard Taqman procedures35 (link) and methods similar to those fully detailed in our previous publications.18 (link),19 (link)
Reed C., Stafford A.M., Mootz J.R., Baba H., Erk J, & Phillips T.J. (2020). A breeding strategy to identify modifiers of high genetic risk for methamphetamine intake. Genes, brain, and behavior, 20(2), e12667.
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3

Enantioselective Effects of Baclofen on Alcohol Intake

Cited 2 times
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(+)-MA hydrochloride (Sigma-Aldrich, St. Louis, MO, USA; Cat. No. M8750) was dissolved in tap water for oral consumption. R(+)-BAC hydrochloride was purchased from Sigma-Aldrich (Cat. No. G013) and dissolved in sterile .9% saline (Baxter Healthcare Corp., Deerfield, IL, USA) for intraperitoneal (IP) injection at a volume of 10 ml/kg body weight. We chose R(+)-BAC because it has been shown to be more potent than S(−)-BAC (Falch et al. 1986 (link); Witczuk et al. 1980 (link)), and one lab demonstrated enantioselective effects of BAC, in which R(+)-BAC reduced EtOH intake, while S(−)-BAC increased EtOH intake (Kasten and Boehm 2014 (link); Kasten et al. 2015 (link)).
Stafford A., Reed C, & Phillips T. (2020). Non-genetic Factors that Influence Methamphetamine Intake in a Genetic Model of Differential Methamphetamine Consumption. Psychopharmacology, 237(11), 3315-3336.
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4

Quantification of Spontaneous Taar1 Mutation

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(+)MA hydrochloride was purchased from Sigma (St. Louis, MO USA) and dissolved in tap water for drinking or in sterile 0.9% saline (Baxter Healthcare Corp., Deerfield, IL, USA) for injection. For CTA studies, sodium chloride (NaCl) was obtained from Sigma (St Louis, MO, USA) and 11.7 g of NaCl was dissolved in 1 liter of tap water to achieve a 0.2M solution. DNA samples used to narrow down the time when the spontaneous mutation arose were extracted from frozen spleen tissue archived at The Jackson Laboratory for DBA/2J mice from generations 208, 218, 221, and 225. These samples were sequenced locally for the Taar1 SNP at position 229. Additional samples from generations 215 and 216 were sequenced by the technical division at The Jackson Laboratory, using our procedures (described below).
Reed C., Baba H., Zhu Z., Erk J., Mootz J.R., Varra N.M., Williams R.W, & Phillips T.J. (2018). A Spontaneous Mutation in Taar1 Impacts Methamphetamine-Related Traits Exclusively in DBA/2 Mice from a Single Vendor. Frontiers in Pharmacology, 8, 993.
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5

Preparation of MA and CEF Solutions

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(+) MA hydrochloride was purchased from Sigma-Aldrich (St. Louis, MO). CEF (Sandoz Inc., Princeton, NJ) was purchased from The University of Toledo Medical Center Pharmacy. Both drugs were dissolved in saline solution (0.9% NaCl). EtOH (190 proof, Decon Lab) was diluted with water for oral gavage.
Alshehri F.S., Althobaiti Y.S, & Sari Y. (2016). Effects of Administered Ethanol and Methamphetamine on Glial Glutamate Transporters in Rat Striatum and Hippocampus. Journal of molecular neuroscience : MN, 61(3), 343-350.
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6

Adolescent Methamphetamine and Nicotine Exposure

Cited 2 times
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(+)-MA hydrochloride (Sigma Aldrich, St. Louis, MO, USA) and (-)-NIC tartrate (Sigma Aldrich, St. Louis, MO, USA) were diluted in 0.9% injectable saline to appropriate concentrations. Intra-peritoneal (IP) injections (0.1 mL) were administered four times daily at two-hour intervals to all mice on PND 30 and PND 31 (early adolescence; [6 (link)]). Treatment groups consisted of MA (7.5 mg/kg × 4 injections), NIC (0.3 mg/kg × 4 injections), MA and NIC (7.5 mg/kg and 0.3 mg/kg, respectively × 4 injections), and saline (× 4 injections). This injection schedule and paradigm was chosen to replicate previous research examining the effects of early adolescent MA exposure in mice [20 (link), 40 (link)] and all solutions were administered via IP injections for consistency between groups. All injections were counterbalanced within the housing cages.
Buck J.M., Morris A.S., Weber S.J., Raber J, & Siegel J.A. (2017). Effects of adolescent methamphetamine and nicotine exposure on behavioral performance and MAP-2 immunoreactivity in the nucleus accumbens of adolescent mice. Behavioural brain research, 323, 78-85.
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7

Methamphetamine Hydrochloride Nebulization

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MA hydrochloride (catalog #M8750-5G, Sigma-Aldrich Corp) was dissolved in water at a concentration of 1 mg/ml and nebulized for experimental animals. For control animals, water was nebulized. A volume of 7.5 ml of nebulized MA or water was expressed from the nebulizer into the chamber during the 1-h interval of availability each day.
Juárez-Portilla C., Pitter M., Kim R.D., Patel P.Y., Ledesma R.A., LeSauter J, & Silver R. (2018). Brain Activity during Methamphetamine Anticipation in a Non-Invasive Self-Administration Paradigm in Mice. eNeuro, 5(2), ENEURO.0433-17.2018.
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8

Nicotine and Methamphetamine Administration

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(−)-Nicotine hydrogen tartrate and (±)-MA hydrochloride (Sigma-Aldrich, St. Louis, MO) were dissolved in saline. Nicotine injections were administered subcutaneously (SC), whereas MA injections were administered intraperitoneally (IP). Both drugs were dissolved in saline and the pH of the nicotine solution was adjusted to 7.4. Nicotine doses were expressed as the free base. For the drinking solutions, MA and sucrose were dissolved in distilled water.
Harmony Z.R., Alderson E.M., Garcia-Carachure I., Bituin L.D, & Crawford C.A. (2020). Effects of Nicotine Exposure on Oral Methamphetamine Self-Administration, Extinction, and Drug-Primed Reinstatement in Adolescent Male and Female Rats. Drug and alcohol dependence, 209, 107927.
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9

Administering Methamphetamine Hydrochloride

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(+)MA hydrochloride was purchased from Sigma (St. Louis, MO, USA) and dissolved in tap water for drinking or in sterile 0.9% saline (Baxter Healthcare Corp., Deerfield, IL, USA) for injection. For the body temperature studies, saline and MA were administered by intraperitoneal injection at a volume of 10 ml/kg body weight.
Stafford A.M., Reed C., Baba H., Walter N.A., Mootz J.R., Williams R.W., Neve K.A., Fedorov L.M., Janowsky A.J, & Phillips T.J. (2019). Taar1 gene variants have a causal role in methamphetamine intake and response and interact with Oprm1. eLife, 8, e46472.
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