5 0 prolene

The MSCs-sponge complex was built as previously described (14 (link)). MSCs were suspended in growth medium at 1×10⁶ cells/ml, and 0.5 ml was seeded onto sterilized 2×10 mm collagen sponge scaffolds. Cell-seeded scaffolds were placed in culture dishes and incubated for 2 h in a minimum volume of normal medium (Fig. 1A), after which more medium was applied to submerge the scaffolds for 24 h. Then, the MSCs-sponge complex was cultured for an additional 7 days using 10 ng/ml of BMP-12 or normal medium.
Animals were anesthetized with 0.3% pentobarbital sodium, and the abdominal skin was sterilized with iodophor. A longitudinal midline skin incision was made to expose the linea alba, and a 10 mm long defect was created along the linea alba (Fig. 1B), referring to a previous study (15 (link)). Then, the linea alba incision was closed using 5-0 prolene (Ethicon, Inc., Cincinnati, OH, USA) with or without the MSCs-sponge complex (Fig. 1C). The suture/incision ratio was 4:1.
After the operation, the animals were raised for another 4 weeks and then euthanized. The whole abdominal wall was obtained for further analysis (Fig. 1D). The sample including the linea alba scar was pruned into two parts with widths of 5 mm. One piece was preserved in saline for tensiometric testing, and the other was fixed in 10% neutral buffered zinc-formalin for histological analysis.

PA injury is defined as significant bleeding from the PA that requires advanced treatment such as compression hemostasis or more in our institute. When bleeding from the PA was identified, the bleeding point was initially gently compressed with lung parenchyma or a cotton stick for several minutes to achieve pressure hemostasis. If the bleeding could not be controlled, a thrombostatic sealant (TachoSil™, CSL Behring), which is a collagen patch coated with human fibrinogen and thrombin, was cut to the appropriate size and subsequently introduced into the thorax through a port and then attached to the bleeding point for a few minutes in conjunction with sponge compression. If two attempts with the TachoSil™ were unsuccessful, or total blood loss reached over 600 ml, then the VATS approach was converted to a thoracotomy, with the operating surgeon determining whether vascular clamps were required to control the massive bleeding. Once the thoracotomy began and the bleeding point was exposed, this was followed by direct suturing of the injured vessels with 5–0 prolene™ (Ethicon) when needed after proximal and distal clamping of the injured PA. The primary cause of the PA injury was verified by three surgeons reviewing the intraoperative video recording postoperatively.

The pulmonary trunks were sutured onto the polyethylene terephthalate fabric lining of their respective pulmonary trunk holder with continuous suture using 5–0 Prolene^® (Ethicon, Somerville, NJ, USA). The pulmonary trunk was subsequently mounted between the ventricular chamber and the compliance chamber on the simulator.

Five cryptotia deformities in four patients were corrected using Z-plasty and ADM graft. The central arm of the Z-plasty was positioned in the inferoposterior direction from the posterior edge of eminentia cymba conchae to the hairline. The anterior line was drawn downwards along the posterior margin of the ear and the posterior line was drawn along the hairline. Next, local anesthetic was injected into the operative field. The incision was made and the superior auricular muscle was detached from the helix. After this step, the skin flap was elevated. Additionally, the skin flap behind the ear was elevated above the cartilage. The posterior skin flap is relatively easy to dissect as it is loosely attached to the cartilage compared to the anterior skin of the ear. All the tissue, including abnormally inserted muscle, was dissected from the ear cartilage. The cartilage abnormality was then observed and ADM was carved as a wedge shape. The matrix was then grafted at the posterior auricular sulcus with 5-0 Prolene (Ethicon, Somerville, NJ, USA) (Fig. 1). The symmetry of projection of the ears was confirmed and a 200 mL Hemovac drain was inserted under the skin flap to prevent seroma and hematoma formation. The Z-plasty was then completed.