Surface protein expression was analyzed using flow cytometry. Cells were harvested and prepared as a single cell suspension. The cells were stained with fluorescence conjugated antibodies (1:50 dilution). The antibodies were FITC conjugated anti-human CD44 (Cat. No. 555478, BD Bioscience, USA), PE-conjugated anti-human CD105 (Cat. No. 21271054, Immuno Tools, Germany), and PerCP-conjugated anti-CD45 (Cat. No. 21810455, Abcam, USA). The mean fluorescence intensity was determined by a FACSCalibur flow cytometer using CellQuest software (BD Bioscience, USA).
Percp conjugated anti cd45
Manufactured by Immunotools
Sourced in Germany
PerCP-conjugated anti-CD45 is a fluorescently labeled antibody that binds to the CD45 surface antigen. CD45 is a pan-leukocyte marker expressed on all human leukocytes. The PerCP fluorescent dye is used to label the antibody, allowing for the detection and identification of CD45-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Facscalibur flow cytometer, by BD (2 mentions)
Cellquest software, by BD (2 mentions)
Pe conjugated anti human cd105, by Immunotools (2 mentions)
Apc conjugated anti human cd90, by Immunotools (2 mentions)
Fitc conjugated anti human cd44, by BD (2 mentions)
Pe conjugated anti human cd105, by BD (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Fascalibur, by BD (1 mentions)
3 protocols using percp conjugated anti cd45
1
Flow Cytometry Analysis of Surface Markers
2
Multiparametric Flow Cytometry Analysis
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Cells were harvested using trypsin/EDTA solution to obtain a single cell suspension. The cells were immunostained in 1% horse serum (Gibco) in sterile phosphate buffered saline (PBS) with primary antibodies conjugated with fluorescent dye. The antibodies were PerCP-conjugated anti-CD45 (Immuno Tools, Friesoythe, Germany), FITC conjugated anti-human CD44 (BD Bioscience Pharmingen, NJ, USA), PE-conjugated anti-human CD105 (Immuno Tools), and APC-conjugated anti-human CD90 (Immuno Tools). Flow cytometry analysis was performed using a FACSCalibur Flow cytometer (BD Bioscience, CA, USA).
3
Immunophenotyping of Stem Cells
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Cells were detached with trypsin/EDTA solution to obtain single-cell suspension. Further, cells were washed with 1% FBS in PBS and subsequently stained with antibodies. Primary antibodies were FITC conjugated anti-human CD44 (BD Bioscience Pharmingen, USA), APC-conjugated anti-human CD90 (Immuno Tools, Germany), PE-conjugated anti-human CD105 (Immuno Tools) and PerCP-conjugated anti-CD45 (Immuno Tools). Stained cells were analysed using a FASCalibur using the CellQuest software (BD Bioscience, USA).
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