Blasticidin hydrochloride

Manufactured by Thermo Fisher Scientific

Blasticidin hydrochloride is a laboratory reagent used as a selection marker in cell culture and molecular biology experiments. It is an antibiotic that acts by inhibiting protein synthesis, allowing for the selection of cells that have been successfully transfected or transduced with a gene of interest.

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Close spelling variants of this product

Blasticidin S-hydrochloride Blasticidin

2 protocols using blasticidin hydrochloride

Cell Culture Media Procurement

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McCoy’s 5A and Dulbecco’s Modified Eagle Medium-F12 (DMEM-F12) were obtained from Thermo Fisher Scientific (Waltham, MA). Hygromycin B, zeocin, blasticidin hydrochloride, and doxycycline hydrochloride also were from Thermo Fisher Scientific. HyClone fetal bovine serum was obtained from GE Healthcare Life Sciences (Pittsburgh, PA). Zinc chloride was obtained from Sigma (St. Louis, MO).

Blomain E.S., Rappaport J.A., Pattison A.M., Bashir B., Caparosa E., Stem J., Snook A.E, & Waldman S.A. (2020). APC-β-catenin-TCF signaling silences the intestinal guanylin-GUCY2C tumor suppressor axis. Cancer Biology & Therapy, 21(5), 441-451.

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Intestinal Organoid Culture Protocol

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McCoy’s 5A (#10050CV), Dulbecco’s Modified Eagle Medium (DMEM) (#10013CV), DMEM/F12 (#10092CV), Matrigel (#354230), and cell recovery solution (#354253) were from Corning (Corning, NY). Ca²⁺/Mg²⁺-free Hank’s Balanced Salt Solution (HBSS) (#14175095), GlutaMax supplement (#35050061), penicillin-streptomycin (#15140122), HEPES (#15630080), HyClone fetal bovine serum (#SH3007103), hygromycin B (#10687010), zeocin (#R25005), blasticidin hydrochloride (#A1113903), geneticin/G418 sulfate (#10131027), doxycycline hydrochloride (#BP2653-1), puromycin (#A1113803), N-2 supplement (#17502048), and B-27 supplement (#12587010) were from Thermo Fisher Scientific (Waltham, MA). N-acetyl-l-cysteine (#A9165), A83-01 (#SML0788), SB202190 (#S7067), nicotinamide (#NO636), and zinc chloride (#Z0152) were from Sigma-Aldrich (St Louis, MO). Wnt3a-, R-Spondin-, and Noggin- conditioned medium (from L-WRN cells; #CRL-3276) was from the American Type Culture Collection (Manassas, VA). Mouse recombinant epidermal growth factor (EGF) (#315-09) and Noggin (#250-38) were from PeproTech (Rocky Hill, NJ). Human recombinant R-spondin-1 (#4645-RS-025/CF) was from R&D Systems (Minneapolis, MN). CHIR99021 (#129830-38-2) and Y-27632 (#252917-06-9) were from StemCell Technologies (Vancouver, Canada).

Rappaport J.A., Entezari A.A., Caspi A., Caksa S., Jhaveri A.V., Stanek T.J., Ertel A., Kupper J., Fortina P.M., McMahon S.B., Jaynes J.B., Snook A.E, & Waldman S.A. (2021). A β-Catenin-TCF-Sensitive Locus Control Region Mediates GUCY2C Ligand Loss in Colorectal Cancer. Cellular and Molecular Gastroenterology and Hepatology, 13(4), 1276-1296.

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