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2 protocols using plain microscope slides

1

Liquid Crystal Compound Synthesis

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The following LC monomers were purchased from Jiangsu Hecheng Advanced Materials Co., Ltd.: 5CB, 8CB, E7, and RM257. The following chemical compounds were purchased from Sigma-Aldrich: water-soluble dyes (EO, rhodamine B, methyl orange, and methylene blue), SDS, DTAB, DMOAP (42 wt % in methanol), photoinitiator 2,2-dimethoxy-2-phenylacetophenone (DMPAP), salts (CaCl2, Na2S, AgNO3, PbNO3, CuSO4, CdCl2, and FeCl3), poly(pyromellitic dianhydride-co-4,4′-oxydianiline) amic acid (PAA), and 1-methyl-2-pyrrolidinone. Azobenzene was purchased from Fisher Scientific. Anhydrous ethanol was purchased from Decon Labs Inc. TEM grids (G75-Au; 3.05 mm in diameter) were purchased from Electron Microscopy Sciences. Water used in all experiments was purified using a Milli-Q water purification system (Simplicity, C9210). Plain microscope slides (25 mm by 75 mm by 1 mm) were purchased from Thermo Fisher Scientific. Unless stated otherwise, purchased chemicals were used as received without further modification or purification.
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2

Nanomaterial Characterization Protocol

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Sodium phosphate dibasic heptahydrate (ACS
grade, 98.0–102.0%), sodium phosphate monobasic monohydrate
(ACS grade, 98.0–102.0%), sodium chloride (ACS grade, ≥99.0%),
11-mercaptoundecyl-N, N,N-trimethylammonium
bromide (HS(CH2)11N(CH3)3Br), Trizma base (NH2C(CH2OH)3,
≥99.8%), 12-mercaptododecanoic acid (HS (CH2)11COOH, 96%), 1-dodecanethiol (HS(CH2)11CH3, ≥98%), and 11-mercapto-1-undecanol (HS(CH2)11OH, ≥99%) were purchased from Sigma-Aldrich
(St. Louis, MO). N-hydroxysulfosuccinimide (NHS),
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC),
and plain microscope slides (25 × 75 × 1 mm) were purchased
from ThermoFisher Scientific (Waltham, MA). CSG10/Au probes were purchased
from NT-MDT Spectrum Instruments (Moscow, Russia). Ethanol 200 proof
was purchased from Decon Labs. Inc. (King of Prussia, PA). All aqueous
solutions or buffers were prepared using purified water with a resistivity
of ≥18 MΩ·cm from a nanopure filtration system (Thermo
Scientific) and filtered using a 0.2 μm disposable bottle top
filter before use. AAV2 and AAV8 empty and full capsids were purchased
from Vigene Biosciences Inc., Rockville, MD. Full capsids contained
the GFP gene.
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