Centaur xpt

Patients’ data were collected by careful reviewing of their electronic medical records. We registered baseline characteristics, cardiovascular risk factors, medical history, medications, ECG, routine biological analyses, and data from coronary angiography and ventriculography. Multiplane coronary angiography was performed in all patients using the standard techniques. Left ventricular ejection fraction (LVEF) was assessed using ventriculography results and 2D-echocardiography according to the Simpson biplane method. Right ventricle involvement was defined by a segmental wall motion alteration of the right ventricle. Three patterns of quantitative TnI and BNP were identified: at admission, at peak, and at discharge. The ratios of BNP/TnI were computed using the first concomitantly available levels of TnI and BNP. Blood samples were analyzed using the Access 2 (Beckman) analyzer in the Colmar center and the Centaur XPT (Siemens) in the Strasbourg center.

Trained study personnel administered a standard questionnaire to collect information on lifestyle characteristics and medications. Anthropometric parameters included body weight and height and were measured in accordance with a standard protocol. Body mass index (BMI) was calculated as weight in kilograms divided by height in meters squared (kg/m²). To avoid interference with circadian rhythm, participants were asked to fast, and blood samples were taken between 8:00 AM and 10:00 AM. All samples were shipped under cold chain management to a central laboratory (certified by the College of American Pathologists) and centrifuged within 4 h.
Levels of metals in blood were measured by inductively coupled plasma mass spectrometer (ICAP-RQ, Thermo Fisher Scientific, Waltham, USA). Thyroid function was tested by chemiluminescence immunoassay (Centaur XPT, Siemens, Erlangen, Germany). TPOAb was assessed using a UniCel Dxl800 (Beckman, Brea, USA), and TgAb with a Biolumi 8000 (SNIBE, Shenzhen, China). Blood lipid profiles were conducted by BS800 (Mindray, Shenzhen, China) and included the following: total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), and low-density lipoprotein (LDL). Glycated hemoglobin (HbA1c) was measured with an HLC-723G8 (TOSOH, Tokyo, Japan).

A venous blood sample was collected prior to the first transfusion of packed red cells. Hematologic analysis was performed using an automated blood cell analyzer (Sysmex XN 1000 A), and Hb analysis was confirmed by high-performance liquid chromatography (HPLC) (Bio-Rad, USA) at the Department of Clinical Pathology, Dr. Hasan Sadikin General Hospital. Chemical immunoassay (Reagent, Siemens EXL 200 Dimension, USA) was used to measure liver function (AST and ALT), while particle-enhanced turbidimetric immunoassay (Dimension, USA) for CRP and ELISA (Elabscience, USA) for IL-6 levels. Iron status was measured by using enzyme immunoassays (Centaur XPT, Siemens, USA), while serum hepcidin levels were measured by human hepcidin using competitive enzyme-linked immunosorbent assay (C-ELISA) (R&D Systems, Minneapolis, MN, USA). The measurement of GDF15 levels was performed by using the quantitative sandwich enzyme immunoassay technique method (ELISA–Quantikine, human GDF15 immunoassay from R&D Systems, Inc., USA). The normal range for GDF15 is 289–1,096 pg/mL, and hepcidin is 12.5–400 ng/mL. The normal range for CRP is 0–6 mg/L, IL-6 is 0–16.4 pg/mL, ALT is 5–45 U/L, and AST is 20–63 U/L.