After shRNA transfection for 72 h, the apoptotic cells were quantified using the Annexin-V/Propidium Iodide (PI) apoptosis kit (Multiscience, Hangzhou, China). SMMC-7721 cells were collected, washed with phosphate-buffered saline (PBS), and resuspended in 200 μl of binding buffer containing 5 μl of annexin V (10 μg/ml) for 10 min in the dark. The cells were then incubated with 10 μl of PI (20 μg/ml), and the samples were immediately analyzed using flow cytometry (Epics XL; Beckman Coulter, Brea, CA, USA). Data acquisition and analysis were performed using CellQuest software.
Annexin 5 propidium iodide apoptosis kit
Manufactured by MultiSciences Biotech
Sourced in China
The Annexin-V/Propidium Iodide (PI) apoptosis kit is a laboratory tool used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin-V, a protein that binds to phosphatidylserine, and Propidium Iodide, a DNA-binding dye, to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells through flow cytometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Epics xl, by Beckman Coulter (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Trizol rna extraction reagent, by Thermo Fisher Scientific (1 mentions)
Tunel reagent kit, by Roche (1 mentions)
Lipofectamine transfection reagent, by Thermo Fisher Scientific (1 mentions)
Sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions)
Rnasin, by Promega (1 mentions)
Facscanto 2 platform, by BD (1 mentions)
Horseradish peroxidase hrp conjugated anti rabbit antibody, by Cell Signaling Technology (1 mentions)
Electrochemiluminescence ecl reagent, by Merck Group (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
4 protocols using annexin 5 propidium iodide apoptosis kit
1
Quantifying Apoptosis via Annexin-V/PI Assay
2
Apoptosis Profiling and Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TRIzol RNA extraction reagent (Invitrogen, Waltham, MA, USA), RNasin (Promega, Madison, WI, USA), fetal calf serum (Gibco, Waltham, MA, USA), annexin V/propidium iodide (PI) apoptosis kit (MultiSciences Biotech, Hangzhou, China), SYBR Green PCR Master Mix (purchased from Applied Biosystems), Lipofectamine transfection reagent (Invitrogen), antifade mounting medium and 4',6-diamidino-2-phenylindole (DAPI)-staining solution (Sigma-Aldrich, St. Louis, MO, USA), DL2000DNA marker (Dalian Proteri Biotechnology Co. Ltd., China), terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) reagent kit (Roche, Basel, Switzerland).
3
Annexin V/PI Apoptosis Assay for SKM-1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The apoptosis rate of treated skm-1 cells was evaluated using an annexin
V/propidium iodide (PI) apoptosis kit from MultiSciences Biotech (Zhejiang,
China) according to the manufacturer’s protocol. For each drug concentration, 1
to 3 × 106 cells were used on the BD FACSCanto II platform (BD
Biosciences, San Jose, CA, USA). Intact cells were marked as annexin
V–/PI–, whereas early apoptotic cells and late
apoptotic/necrotic cells were marked as annexin V+/PI– and
annexin V+/PI+, respectively.
V/propidium iodide (PI) apoptosis kit from MultiSciences Biotech (Zhejiang,
China) according to the manufacturer’s protocol. For each drug concentration, 1
to 3 × 106 cells were used on the BD FACSCanto II platform (BD
Biosciences, San Jose, CA, USA). Intact cells were marked as annexin
V–/PI–, whereas early apoptotic cells and late
apoptotic/necrotic cells were marked as annexin V+/PI– and
annexin V+/PI+, respectively.
4
Apoptosis Pathway Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
4,5-Dimethylthiazol-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) was bought from MP Biomedicals (California, USA). Annexin V/propidium iodide (PI) apoptosis kit was purchased from MultiSciences Biotech Co., Ltd (Hangzhou, China). BCA protein assay kit was bought from Thermo Fisher Scientific, Inc (ML, USA). Rabbit anti-human PIK3CA, EGFR, phospho-EGFR (p-EGFR), AKT, p-AKT, ERK, and p-ERK monoclonal antibodies (mAb) and horseradish peroxidase (HRP) conjugated anti-rabbit antibody were purchased from Cell Signaling Technology, Inc (MA, USA). Electro-Chemi-Luminescence (ECL) reagent was purchased from Millipore Corporation (MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!