Fish were maintained in the WT(ABb) background at 28.5°C. Lines used were Tg(sp7:EGFP)b1212 abbreviated sp7:EGFP (DeLaurier et al., 2010 (link)), Et(krt4:EGFP)sqet37 abbreviated ET37:EGFP (Parinov et al., 2004 (link)), Tg(sp7:nEOS)vp46rTg (Aman et al., 2021 (link)), bnc2utr16e1 (Lang et al., 2009 (link)), edadt1261 (Harris et al., 2008 (link)), Tg(tg:nVenus-v2a-nfnB)wprt8Tg abbreviated tg:Venus-NTR, Tg(tyrp1b:palm-mCherry)wprt11Tg (McMenamin et al., 2014 (link)), and Tg(pnp4a:palm-mCherry)wprt10Tg. Thyroid ablation and rearing of hypoTH fish were done as previously described (McMenamin et al., 2014 (link)). csf1a and csf1b mutant fish were generated by injecting CRISPR/Cas9 reagents (PNAbio) including synthetic single-guide RNA targeting the genomic sequences (csf1a: 5′-GCGGCATTCCCTCACATAC ; csf1b: 5′-GGCATGTTTGCAAGGACCG ) into zygotes, selecting phenotypic F0 animals, and repeat outcrossing to generate F2 families (Hwang et al., 2013 (link)). Recovered alleles contained premature termination codons owing to frame shift mutations (csf1a: 10 bp insertion; csf1b: 2 or 5 bp deletions having phenotypes indistinguishable from one another).
Crispr cas9 reagents
Manufactured by PNA Bio
CRISPR/Cas9 reagents are a set of molecular tools used in gene editing. They consist of a guide RNA and a Cas9 enzyme that together can recognize and cleave specific DNA sequences. The core function of CRISPR/Cas9 reagents is to enable targeted modifications of genetic material.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using crispr cas9 reagents
1
Zebrafish Genetic Toolkit and Thyroid Ablation
2
Zebrafish Genetic Toolkit and Thyroid Ablation
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Fish were maintained in the WT(ABb) background at 28.5°C. Lines used were Tg(sp7:EGFP)b1212 abbreviated sp7:EGFP (DeLaurier et al., 2010 (link)), Et(krt4:EGFP)sqet37 abbreviated ET37:EGFP (Parinov et al., 2004 (link)), Tg(sp7:nEOS)vp46rTg (Aman et al., 2021 (link)), bnc2utr16e1 (Lang et al., 2009 (link)), edadt1261 (Harris et al., 2008 (link)), Tg(tg:nVenus-v2a-nfnB)wprt8Tg abbreviated tg:Venus-NTR, Tg(tyrp1b:palm-mCherry)wprt11Tg (McMenamin et al., 2014 (link)), and Tg(pnp4a:palm-mCherry)wprt10Tg. Thyroid ablation and rearing of hypoTH fish were done as previously described (McMenamin et al., 2014 (link)). csf1a and csf1b mutant fish were generated by injecting CRISPR/Cas9 reagents (PNAbio) including synthetic single-guide RNA targeting the genomic sequences (csf1a: 5′-GCGGCATTCCCTCACATAC ; csf1b: 5′-GGCATGTTTGCAAGGACCG ) into zygotes, selecting phenotypic F0 animals, and repeat outcrossing to generate F2 families (Hwang et al., 2013 (link)). Recovered alleles contained premature termination codons owing to frame shift mutations (csf1a: 10 bp insertion; csf1b: 2 or 5 bp deletions having phenotypes indistinguishable from one another).
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