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Lyoplate mouse cell surface marker screening panel

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The BD Lyoplate™ Mouse Cell Surface Marker Screening Panel is a comprehensive set of pre-formatted 96-well plates containing a collection of antibodies targeting a wide range of mouse cell surface markers. The panel is designed to facilitate the screening and identification of cell surface antigens expressed on mouse cells.

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Lab products found in correlation

Accutase, by BD (2 mentions) Apc conjugated anti human mouse cd49f itga6 antibody, by BioLegend (2 mentions) Guava easy cyte 6ht 2l flow cytometer, by Merck Group (1 mentions) Guava easycyte 6ht 2l ow cytometer, by Merck Group (1 mentions)

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Lyoplate (7 citations) BD Lyoplate™ Screening Panels (6 citations) Mouse Cell Surface Marker Screening Panel (2 citations)

5 protocols using lyoplate mouse cell surface marker screening panel

1

Flow Cytometry of MPC Surface Markers

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To determine surface marker expression of MPC, flow cytometry was performed on a Guava easyCyte 6HT 2 L flow cytometer (Merck Millipore, Germany). The BD Lyoplate™ Mouse Cell Surface Marker Screening Panel containing purified monoclonal antibodies specific for inter alia CD9 and CD98 cell surface marker proteins (BD biosciences, USA) was employed according to manufacturer’s instructions. Cell events were acquired with Guava InCyte™ v.2.3 software. Histograms and dot- plots were generated with a minimum of 5000 events at a sample flow rate of 1.8 μL/mL. Positive staining was obtained by comparison with isotype control set as at least 95% negative or comparison to control (negative) cells.
Messner F., Thurner M., Müller J., Blumer M., Hofmann J., Marksteiner R., Couillard-Despres S., Troppmair J., Öfner D., Schneeberger S, & Hautz T. (2021). Myogenic progenitor cell transplantation for muscle regeneration following hindlimb ischemia and reperfusion. Stem Cell Research & Therapy, 12, 146.
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2

Flow Cytometry Cell Profiling of E0771 Cells

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All cell cultures destined for flow cytometry were dissociated with Accutase (BD Biosciences, USA) to limit cleavage of extracellular receptors. Cells were labeled with the APC-conjugated anti-human/mouse CD49f/ITGA6 antibody (BioLegend, Bethesda, MD, USA, product: 313615) or the APC-conjugated Rat IgG2a,k isotype control (BioLegend, Bethesda, MD, USA, product: 400511) according to manufacturer’s recommendations. The flow cytometry cell surface marker screen was conducted on E0771 cells using the BD Lyoplate Mouse Cell Surface Marker Screening Panel (BD, Franklin Lakes, NJ, USA). The geometric mean of fluorescent values <4 was chosen as the cutoff in accordance with assay negative controls. Values for the entire dataset are contained in Supplementary Table 3.
Berens E.B., Sharif G.M., Schmidt M.O., Yan G., Shuptrine C.W., Weiner L.M., Glasgow E., Riegel A.T, & Wellstein A. (2016). Keratin-associated protein 5-5 controls cytoskeletal function and cancer cell vascular invasion. Oncogene, 36(5), 593-605.
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3

Flow Cytometry Cell Profiling of E0771 Cells

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All cell cultures destined for flow cytometry were dissociated with Accutase (BD Biosciences, USA) to limit cleavage of extracellular receptors. Cells were labeled with the APC-conjugated anti-human/mouse CD49f/ITGA6 antibody (BioLegend, Bethesda, MD, USA, product: 313615) or the APC-conjugated Rat IgG2a,k isotype control (BioLegend, Bethesda, MD, USA, product: 400511) according to manufacturer’s recommendations. The flow cytometry cell surface marker screen was conducted on E0771 cells using the BD Lyoplate Mouse Cell Surface Marker Screening Panel (BD, Franklin Lakes, NJ, USA). The geometric mean of fluorescent values <4 was chosen as the cutoff in accordance with assay negative controls. Values for the entire dataset are contained in Supplementary Table 3.
Berens E.B., Sharif G.M., Schmidt M.O., Yan G., Shuptrine C.W., Weiner L.M., Glasgow E., Riegel A.T, & Wellstein A. (2016). Keratin-associated protein 5-5 controls cytoskeletal function and cancer cell vascular invasion. Oncogene, 36(5), 593-605.
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4

Multi-Marker Phenotyping of Dermal Fibroblasts

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Dermal fibroblasts were harvested as described previously from adult
(P56) En1Cre;R26mTmGtransgenic mice. Cells were analyzed using BD Lyoplate Mouse Cell Surface Marker
Screening Panel (BD Biosciences, cat. 562208) containing 176 purified monoclonal
antibodies and corresponding isotype controls. Manufacturer's staining protocol
was followed with slight modifications. Cells isolated from the dorsal dermis of
En1Cre;R26mTmGmice were plated into U-bottom 96-well plates at a density of 2.5 to 5 ×
105 cells per well in FACS buffer. Primary antibody incubation
was done in 100-μl volume for 30 min on ice. Next, cells were incubated
with biotinylated secondary antibodies (goat antimouse 1:400, goat antirat
1:400, goat antisyrian hamster 1:400, goat anti–Armenian hamster 1:800)
in 100-μl volume for 30 min on ice. Tertiary incubation with Alexa Fluor
647 Streptavidin (1:4000)—as well as PacBlue-conjugated CD31 (1:100),
CD45 (1:200)—and Ter-119 (1:200), was carried out in 100-μl volume
for 30 min on ice. Analysis was performed using flow cytometer BD LSR Fortessa
with High Throughput Sampler.
Rinkevich Y., Walmsley G.G., Hu M.S., Maan Z.N., Newman A.M., Drukker M., Januszyk M., Krampitz G.W., Gurtner G.C., Lorenz H.P., Weissman I.L, & Longaker M.T. (2015). Identification and isolation of a dermal lineage with intrinsic fibrogenic potential. Science (New York, N.Y.), 348(6232), aaa2151.
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5

Flow Cytometry Analysis of MPC

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To determine surface marker expression of MPC, ow cytometry was performed on a Guava easyCyte 6HT 2L ow cytometer (Merck Millipore, Germany). The BD Lyoplate™ Mouse Cell Surface Marker Screening Panel containing puri ed monoclonal antibodies speci c for inter alia CD9 and CD98 cell surface marker proteins (BD biosciences, USA) was employed according to manufacturer's instructions. Cell events were acquired with Guava InCyte™ v.2.3 software. Histograms and dot-plots were generated with a minimum of 5000 events at a sample ow rate of 1.8 μL/mL. Positive staining was obtained by comparison with isotype control set as at least 95% negative or comparison to control (negative) cells.
Messner F., Thurner M., Müller J., Blumer M., Hofmann J., Marksteiner R., Couillard‐Després S., Troppmair J., Öfner D., Schneeberger S., & Hautz T. (2021). Myogenic progenitor cell transplantation for muscle regeneration following hindlimb ischemia and reperfusion.
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