Anti stat3 12640

WXWH0265 was provided by WuXi AppTec Group (Shanghai, China) and prepared in vivo in 5% dimethyl sulphoxide (DMSO) and 95% double‐distilled water. The DMSO concentration was limited to 0.1% in vitro. The signal transducer and activator of transcription 3 (STAT3) inhibitors, napabucasin (BBI608), fasudil (HY‐10341A), pirfenidone (HY‐B0673) and GSK429286A (HY‐11000), were purchased from MedChemExpress. S7936, Belumosudil (KD025) was purchased from Selleck Chemicals, USA. Clodronate liposomes were obtained from Liposomes (CP‐005‐005). Anti‐STAT3 (# 12640) and anti‐p‐STAT3 (Y705; # 9145) antibodies were purchased from Cell Signaling Technology (Cambridge, MA, USA). Anti‐α‐SMA (# MAB1420) antibodies were obtained from R&D Systems (Minneapolis, MN, USA). Anti‐ROCK1 (Cat# ab134181), anti‐ROCK2 (Cat# ab71598) and anti‐collagen‐Ⅰ (Cat# ab138492) antibodies were purchased from Abcam (Cambridge, UK). Anti‐β‐tubulin antibodies were purchased from Huabio Technology (Beijing, China).

The following antibodies were used in the study: anti-Fbw7 (for IHC) (H00055294-M02, Abnova, Taipei City, Taiwan), anti-Fbw7 (for western blots) (ab109617, Abcam, Cambridge, UK), anti-Stat3, 12640; anti-phospho-Stat3^Tyr705, 4113; anti-Ubiquitin, 3936 (Cell Signaling Technology, Beverly, MA, USA), anti-Myc tag, 16286–1-AP; anti-Flag tag, 66008–2-Ig; and anti-β-actin, 60008–2-Ig; anti-Myc, 10828–1-AP; anti-Notch, 10062–2-AP; anti-Jun, 10024–2-AP; anti-DEK, 16448–1-AP; anti-Mcl1, 16225–1-AP(Proteintech, Rosemont, IL, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Amresco (Dallas, TX, USA).