Ldc4297

Antiviral drugs were obtained from the following sources: Calbiochem, Darmstadt, Germany (CDK2 Inh II, Gö6976); GPC Biotech AG, Martinsried, Germany (R25); Lead Discovery Center GmbH, Dortmund, Germany (LDC4297); MedChemExpress, Monmouth Junction, NJ, USA (abemaciclib, AZD4573, CVT-313, CYC065, dinaciclib, maribavir, riviciclib, samuraciclib SEL120, SY1365); Tocris, Wiesbaden-Nordenstadt, Germany (SNS032, THAL-SNS032); Vichem Kft, Budapest, Hungary (Ax7396, Vi7392). Stock aliquots were prepared in sterile DMSO (Sigma Aldrich, St. Louis, MO, USA) and stored at −20 °C.

ATPγS was purchased from Sigma Aldrich; THZ1 was purchased from EMD Millipore; LDC4297 and dinaciclib were purchased from MedChemExpress (distributed by Insight Biotechnology, Wembley, UK). ICEC-series pyrazolopyrimidine compounds were synthesized and characterized during the course of the drug discovery program that led to the discovery of ICEC0942^{5 (link),30 (link)–33 ,41} . Further characterization data for ICEC0510-R and S enantiomers, ICEC0768, ICEC0880, ICEC0914, and CT7030 are provided above. After retrieval from cold storage, integrity of ICEC-series compounds was verified using UV-vis spectroscopy and liquid chromatography/mass spectrometry (see below for detailed methods). The results of this characterization are summarized in Supplementary Table 12.