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Ab992

Manufactured by Merck Group

Ab992 is a piece of laboratory equipment designed for general use in research and testing environments. It is a precise and reliable tool for performing a variety of tasks, such as sample preparation, analysis, and measurement. The core function of Ab992 is to provide accurate and consistent results, enabling researchers and scientists to conduct their work efficiently and effectively.

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3 protocols using ab992

1

Chromatin Immunoprecipitation Protocol

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Antibodies to histone H3K4me3 (Abcam ab8580), histone H3K27ac (Abcam ab4729), histone H2A.z (Abcam ab4174), histone H3K27me3 (Abcam ab6002), CTCF (Millipore 07–729), Rad21 (Abcam ab992), Calbindin (Millipore AB1778), GFP (Abcam ab13970), and DsRed (Clontech 632496) were purchased.
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2

Chromatin Remodeling Complex Protein Detection

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Primary antibodies for Western blots used were rabbit anti-human SNF2H (Bethyl Laboratories, A301-081A), mouse anti-CTCF (Abcam, ab37477), rabbit anti-RAD21 (Abcam, ab992), rabbit anti-CHD1 (Bethyl Laboratories, A301-218A), rabbit anti-CHD2 (Active Motif, 39364), rabbit anti-CHD4 (Bethyl Laboratories, A301-081A), rabbit anti-ACF1 (Bethyl Laboratories, A301-318A), rabbit anti-WSTF (Cell Signaling, 2152), rabbit anti–TIP5 (Invitrogen, 491037) and mouse anti-beta actin (Sigma, A2228). Primary antibodies for ChIP used were rabbit anti-human SNF2H (Abcam, ab72499), mouse anti-CTCF (Millipore, 17–10044), rabbit anti-RAD21 (Abcam, ab992), rabbit anti-BPTF (Millipore, Abe24), and rat anti-SNF2L (2C4, [39 (link)] which has been kindly provided by P. Becker). Secondary antibodies for Western blots used were Alexa Fluor 680 goat anti-mouse (Invitrogen) and Alexa Fluor 790 goat anti-rabbit (Invitrogen) for immunofluorescence staining and analysis using the LI-COR Odyssey CLx.
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3

Chromatin Immunoprecipitation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to histone H3K4me3 (Abcam ab8580), histone H3K27ac (Abcam ab4729), histone H2A.z (Abcam ab4174), histone H3K27me3 (Abcam ab6002), CTCF (Millipore 07–729), Rad21 (Abcam ab992), Calbindin (Millipore AB1778), GFP (Abcam ab13970), and DsRed (Clontech 632496) were purchased.
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