TGF-β was obtained from R&D Systems (Minneapolis, MN, USA). Goat anti-MRTF-A (C-19) and rabbit anti-MRTF-A (H-140) antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA); rabbit anti-palladin, mouse anti-SMA, mouse anti-calponin, mouse anti-β-tubulin, and mouse anti-β-actin antibodies (Sigma, St. Louis, MO, USA); and phospho-Ser/Thr antibody (Abcam, Cambridge, MA) were used for western blot. siNox4, siMRTF-A, siPalladin and All-Star negative control siRNA (siNeg) (Qiagen, Valencia, CA), Lipofectamine RNAiMAX (Invitrogen, Mountain View, CA), and OPTI-MEM (Gibco) were used for siRNA transfection. ROCK inhibitor (Y-27632) (Sigma, St. Louis, MO, USA) was used for kinase inhibition.
Rabbit anti palladin
Manufactured by Merck Group
Sourced in United States
Rabbit anti-palladin is a primary antibody that specifically binds to the palladin protein. Palladin is a cytoskeletal protein involved in the organization and dynamics of the actin cytoskeleton. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to detect and study the expression and localization of palladin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Rock inhibitor y 27632, by Merck Group (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Mouse anti β tubulin, by Merck Group (1 mentions)
Tgf β, by R&D Systems (1 mentions)
Mouse anti sma, by Merck Group (1 mentions)
Mouse anti gapdh, by Merck Group (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Bis tris gel, by Thermo Fisher Scientific (1 mentions)
Protease and phosphatase inhibitor, by Merck Group (1 mentions)
2 protocols using rabbit anti palladin
1
Elucidating MRTF-A Signaling Pathways
2
Western Blot Analysis of Palladin and mDia2
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Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (Sigma) with phosphatase and protease inhibitors (EMD Millipore) and heated to 70°C. Samples were run on a 4–12% Bis–Tris gel (Life Technologies) and transferred to a polyvinylidene fluoride (PVDF) membrane (Life Technologies). The following primary antibodies were used: rabbit anti-palladin (1:1000; Sigma), rabbit anti-mDia2 (1:750; ProteinTech), mouse anti-GAPDH (1:10,000, Sigma). The following secondary antibodies were used: goat anti-mouse horseradish peroxidase (HRP)-conjugate, goat anti-rabbit HRP-conjugate (both from Life Technologies). HRP-conjugated bands were imaged using enhanced chemiluminescence reagent (ECL, Thermo Fisher).
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