A sequential inductively coupled plasma optical emission spectrometer (ICP-OES, OPTIMA 7000, Perkin Elmer) was employed to determine Hg2+. The dry weight of cells was calculated from the OD600 by using the value of 0.292 g dry weight per liter at an OD600 of 1.0 which was obtained in the experiment. Hg2+ binding capacity was expressed as milligram Hg2+ accumulated per gram dry weight of cells (mg/g). Hg2+ removal ratio was expressed as the percentage of Hg2+ removed from Hg2+ aqueous solution (%). XPS analysis was performed by a X-ray photo-electron spectroscopy (Microlab 350, Thermo VG).
Optima 7000
Manufactured by PerkinElmer
Sourced in United States
The PerkinElmer Optima 7000 is an inductively coupled plasma optical emission spectrometer (ICP-OES) system designed for elemental analysis. It provides high-performance capabilities for the detection and quantification of trace and major elements in a wide range of sample types.
Automatically generated - may contain errors
Lab products found in correlation
Milli q apparatus, by Merck Group (2 mentions)
Titrisol, by Merck Group (2 mentions)
Toc tn analyzer multi n c 3000, by Analytik Jena (1 mentions)
2400 chn elemental, by PerkinElmer (1 mentions)
X pert pro diffractometer, by Malvern Panalytical (1 mentions)
Mls 1200 mega, by Milestone (1 mentions)
Nova nanosem 230, by Thermo Fisher Scientific (1 mentions)
Alpha ftir spectrometer, by Bruker (1 mentions)
Usb2000, by OceanOptics (1 mentions)
Pw1710 diffractometer, by Malvern Panalytical (1 mentions)
Cary 630 spectrometer, by Agilent Technologies (1 mentions)
Litesizer 500 particle analyzer, by Anton Paar (1 mentions)
Easypure 2, by Thermo Fisher Scientific (1 mentions)
Jem 1400, by JEOL (1 mentions)
D8 advance diffractometer, by Bruker (1 mentions)
Cbm 20a, by Shimadzu (1 mentions)
Milli q system, by Merck Group (1 mentions)
17 protocols using optima 7000
1
Quantifying Hg2+ Binding Capacity
2
Phosphorus Analysis in Lotus japonicus
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10–20 mg dried leaf material from non-mycorrhized and mycorrhized Lotus japonicus wild-type and lys11 mutant plants were digested in 1 mL 6 M HNO3 at 90 °C with shaking (450 rpm) for 1 hour. The leaf extract was diluted to 6 ml with distilled water and filtered. All experiments were performed in three replicates and blanks (containing no samples) were simultaneously run. Phosphorous concentration was determined with a Perkin Elmer Optima 7000 (Perkin Elmer, Norwalk, Connecticut, USA) inductively coupled plasma-optical emission spectrometer (ICP-OES). The reagents adopted were of analytical grade purity. Metal standard solutions were prepared from concentrated stock solutions (Merck Titrisol). High purity water (HPW) obtained from a Milli-Q apparatus (Millipore, Bedford, USA) was used throughout.
3
Soil Carbon and Mineral Analysis
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The carbon (C) contents in the soil were analyzed using a Perkin-Elmer 2400 CHN elemental analyzer. The filtered (0.45 µm PTFE filter) extract was measured for DOC using a TOC/TN analyzer (multi N/C 3000, Analytik Jena AG, Germany). The pH was determined in 1∶2.5 (w/v) soil:water extracts using a pH electrode. Electrical conductivity (EC) was determined using a conductivity meter (LF91, German). The Al and Si concentrations in the soil FA extracts were measured using ICP-AES (Optima 7000, PerkinElmer, USA). Mineralogical identification was performed using X-ray powder diffraction (Nanjing Normal University). The diffraction patterns were recorded from 3 to 60° 2-theta using Ni-filtered Cu K-alpha radiation at a rate of 300 s per step on a PANalytical X-pert Pro diffractometer using an X-celerator position-sensitive detector.
4
Iron Adsorption Quantification by ICP-OES
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Iron adsorption
was quantified by inductively coupled plasma-optical emission spectroscopy,
ICP OPTIMA 7000 equipped with WinLan 32 software (Perkin-Elmer, Milan,
Italy). The ICP was equipped with a cyclonic spray chamber and a Teflon
Mira Mist nebulizer. The instrumental conditions were plasma power
1.5 kW; sample aspiration rate 15 rpm (∼2 mL min–1); argon nebulizer flow 0.6 L min–1; argon auxiliary
flow 0.2 L min–1; and argon plasma flow 15 L min–1.
The adsorption experiments were performed
starting from 50 mL of a 5.85 ppm Fe3+ aqueous solution,
the concentration of iron detected in chronic wounds in the literature.20 (link) The pH of the solutions was adjusted to 7.6
with HCl 1 M. The adsorption experiments were carried out at room
temperature by dipping 2 mg of C-Ker-film for different times ranging
from 1 to 168 h (1 week). ICP-OES analysis was performed by previously
filtering the solution with a PTFE filter (0.45 μm) and then
adding 1% v/v nitric acid (65% wt). Each concentration value was averaged
based on three measurements. ICP-OES data are expressed in μg
mg–1.
was quantified by inductively coupled plasma-optical emission spectroscopy,
ICP OPTIMA 7000 equipped with WinLan 32 software (Perkin-Elmer, Milan,
Italy). The ICP was equipped with a cyclonic spray chamber and a Teflon
Mira Mist nebulizer. The instrumental conditions were plasma power
1.5 kW; sample aspiration rate 15 rpm (∼2 mL min–1); argon nebulizer flow 0.6 L min–1; argon auxiliary
flow 0.2 L min–1; and argon plasma flow 15 L min–1.
The adsorption experiments were performed
starting from 50 mL of a 5.85 ppm Fe3+ aqueous solution,
the concentration of iron detected in chronic wounds in the literature.20 (link) The pH of the solutions was adjusted to 7.6
with HCl 1 M. The adsorption experiments were carried out at room
temperature by dipping 2 mg of C-Ker-film for different times ranging
from 1 to 168 h (1 week). ICP-OES analysis was performed by previously
filtering the solution with a PTFE filter (0.45 μm) and then
adding 1% v/v nitric acid (65% wt). Each concentration value was averaged
based on three measurements. ICP-OES data are expressed in μg
mg–1.
5
Catalyst Recycling and Characterization
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The catalyst recovered after the filtration of the crude reaction mixture was washed with water (20 mL), acetone (20 mL), and methanol (20 mL), dried under vacuum at room temperature overnight and room temperature, and reused in the next runs under the same optimized reaction conditions. The conversions obtained after the recycling tests were measured by GC-MS. Catalyst structure and Pd loading were also analyzed after recycling.
The total Pd content was determined using ICP–OES with a Perkin Elmer Optima 7000 (Perkin Elmer, Norwalk, CT, USA) spectrometer via standard addition.
The total Pd content was determined using ICP–OES with a Perkin Elmer Optima 7000 (Perkin Elmer, Norwalk, CT, USA) spectrometer via standard addition.
6
Assessing Magnesium and Calcium Ions Release in Phosphate Buffer Saline
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The CPC and Mg-CPCs were made into a cylinder with a diameter of 4.5 mm and a height of 6 mm by using a mold. After setting and incubate at 37°C for 24 h, the cement samples were immersed in an 8 ml phosphate buffer saline (PBS) solution (without calcium and magnesium ions, PH = 7.35) and placed in a 37°C incubator. At days 1, 3, 7, 14 and 28 after immersion, all the extracts were collected and replaced with fresh PBS. An inductively coupled plasma emission spectrometer (Perkin Elmer, Optima 7000, United States) was used to detect the concentration of Mg and Ca ions in the extracted samples. The process was repeated three times for each group of samples.
7
Silver Release Quantification from PCL
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The amount of silver released from the enriched PCL-based constructs over time was monitored via inductively coupled plasma–optical emission spectroscopy (ICP-OES) with a PerkinElmer Optima 7000 DV apparatus (PerkinElmer, Waltham, MA, USA). The instrument was equipped with a PEEK Mira Mist nebulizer, a cyclonic spray chamber, and an Echelle monochromator. A wavelength of 328.068 nm was used for Ag. Each concentration value was calculated using the external calibration method, with standard solutions prepared using the matrix matching method and considering the average value of 3 instrumental measurements.
8
Microwave-assisted metal analysis
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Sample dissolution was performed in polytetrafluoroethylene (PTFE) bombs, with a Milestone MLS-1200 Mega (Milestone, Sorisole, Italy) microwave laboratory unit.
Sample analyses were carried out with a Perkin Elmer Optima 7000 (Perkin Elmer, Norwalk, CT, USA) inductively coupled plasma optical emission spectrometer (ICP-OES).
Analytical grade reagents were used throughout the experiments. Standard metal solutions were prepared from concentrated Merck Titrisol stock solutions (Merck, Darmstadt, Germany).
High purity water (HPW) obtained from a Milli-Q apparatus (Millipore, Burlington, MA, USA) was used for the preparation of samples as well as standard solutions.
Sample analyses were carried out with a Perkin Elmer Optima 7000 (Perkin Elmer, Norwalk, CT, USA) inductively coupled plasma optical emission spectrometer (ICP-OES).
Analytical grade reagents were used throughout the experiments. Standard metal solutions were prepared from concentrated Merck Titrisol stock solutions (Merck, Darmstadt, Germany).
High purity water (HPW) obtained from a Milli-Q apparatus (Millipore, Burlington, MA, USA) was used for the preparation of samples as well as standard solutions.
9
Biocatalytic Enzyme-COF Characterization
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An FEI Nova NanoSEM 230 was used to obtain SEM images of the samples after Pt coating. Crystallinity analysis of biocatalytic enzyme-COF was performed using XRD-MPD-powder diffraction. The samples were vacuum dried and analysed using PXRD measurement. Small-angle X-ray scattering (SAXS) patterns were collected at the Australian Synchrotron using a Pilatus 1 M detector and a 20 keV beam. Fourier transform infrared (FTIR) spectra were recorded on a Bruker Alpha FTIR Spectrometer. Metal ion content in the samples was determined using Inductively coupled plasma-optical emission spectroscopy (ICP-OES) instruments (Optima7000 and Avio from PerkinElmer, USA). Synchrotron Far-infrared (FIR)/terahertz (THz) radiation was collected at the Australian Synchrotron using an attenuated total reflectance (ATR) sampling accessory in the range of 50–750 cm−1.
10
Trace Mineral Analysis in Plasma and Liver
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Plasma and liver samples were analyzed for trace minerals using inductively coupled plasma-optical emissions spectroscopy (ICP-OES; Optima 7000; PerkinElmer, Waltham, MA) using methods described in Pogge and Hansen (2013). Quality control samples (serum UTAK, Valencia, CA; bovine liver from National Institutes of Standards and Technology, Gaithersburg, MD) were included in all runs to verify instrument accuracy.
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