Df1900

Manufactured by R&D Systems

The DF1900 is a laboratory instrument designed for the detection and quantification of analytes in biological samples. It utilizes advanced detection technologies to provide accurate and reliable results. The core function of the DF1900 is to perform various analytical assays, but a detailed description without interpretation or extrapolation cannot be provided within the given parameters.

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Lab products found in correlation

Df2100, by R&D Systems (2 mentions) Hr series nefa hr 2, by Fujifilm (1 mentions) Dpc900, by R&D Systems (1 mentions) Eglp 35k, by Merck Group (1 mentions) Microplate reader, by Molecular Devices (1 mentions) Dla50, by R&D Systems (1 mentions) Dlb50, by R&D Systems (1 mentions) Ab193695, by Abcam (1 mentions) Bio plex system, by Bio-Rad (1 mentions) Luminex xmap technology, by Bio-Rad (1 mentions)

Close spelling variants of this product

FGF19 Quantikine® DF1900 (4 citations)

8 protocols using df1900

Serum Biomarkers for Cholesterol Metabolism

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Serum fibroblast growth factor (FGF)21, proprotein convertase subtilisin/kexin type 9 (PCSK9), and FGF19 levels were determined by ELISA (Catalog nos. DF2100, DPC900, and DF1900, respectively; R&D Systems), according to the manufacturer’s instructions. Serum NEFA levels were determined using an enzymatic colorimetric assay [HR Series NEFA-HR(2); Wako Diagnostics]. Levels of serum BAs and 7a-hydroxy-4-cholesten-3-one (C4), a BA synthesis marker, were determined by liquid chromatography–tandem mass-spectrometry using deuterium-labeled standards for C4 and BA (24 (link)). Unesterified lathosterol, a serum marker of total cholesterol synthesis, was determined by gas chromatography–mass spectrometry (25 (link)). C4 and lathosterol levels were corrected for total serum and total cholesterol (C4c and lathosterol/c), respectively (26 (link), 27 (link)).

Rosqvist F., Kullberg J., Ståhlman M., Cedernaes J., Heurling K., Johansson H.E., Iggman D., Wilking H., Larsson A., Eriksson O., Johansson L., Straniero S., Rudling M., Antoni G., Lubberink M., Orho-Melander M., Borén J., Ahlström H, & Risérus U. (2019). Overeating Saturated Fat Promotes Fatty Liver and Ceramides Compared With Polyunsaturated Fat: A Randomized Trial. The Journal of Clinical Endocrinology and Metabolism, 104(12), 6207-6219.

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Quantification of Circulating FGF19 Levels

Cited 1 time

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Circulation FGF19 levels of the health controls and NTCP p.Ser267Phe variant were determined using a sandwich enzyme-linked immunosorbent assay according to the manufacturer’s instructions (DF1900; R&D Systems, Inc, Minneapolis, MN).¹⁷ (link)

Mao F., Wang M.X., Hou X., Zhou Z., Yan Y.Y., Fang L.J., Tan Z., Fang W.Y., Liu T., He W., Li C., Xie X.B., Lu S.Q., Sui J., Wang F., Han J., Wang J.S, & Li W. (2020). NTCP Deficiency Causes Gallbladder Abnormalities in Mice and Human Beings. Cellular and Molecular Gastroenterology and Hepatology, 11(3), 831-839.

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Quantifying Serum FGF, GLP-1 Levels

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Serum levels of fibroblast growth factor 19 (FGF19) (catalog #DF1900; R&D Systems, Minneapolis, MN), FGF21 (catalog #DF2100; R&D Systems), and glucagon‐like peptide 1 (GLP‐1) (catalog #EGLP‐35K; Millipore, Burlington, MS) were conducted per the manufacturers’ protocols.

Yang Z., Kusumanchi P., Ross R.A., Heathers L., Chandler K., Oshodi A., Thoudam T., Li F., Wang L, & Liangpunsakul S. (2019). Serum Metabolomic Profiling Identifies Key Metabolic Signatures Associated With Pathogenesis of Alcoholic Liver Disease in Humans. Hepatology Communications, 3(4), 542-557.

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Quantification of Protein Biomarkers

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Enzyme‐linked immunosorbent assay (ELISA) kits were used to detect the concentrations of human FGF19 (DF1900, R&D Systems), IL‐1α (DLA50, R&D Systems), IL‐1β (DLB50, R&D Systems) and complement C5a (ab193695, Abcam) in culture supernatants or serum as described.^[³⁶^] The absorbance values could be read on a microplate reader (Molecular Devices) at a wavelength of 450 nm, within 30 min. The standard curve was used to convert absorbance values to protein concentrations.

Li C., Chen T., Liu J., Wang Y., Zhang C., Guo L., Shi D., Zhang T., Wang X, & Li J. (2023). FGF19‐Induced Inflammatory CAF Promoted Neutrophil Extracellular Trap Formation in the Liver Metastasis of Colorectal Cancer. Advanced Science, 10(24), 2302613.

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Quantification of Inflammatory Markers and Vascular Mediators

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Serum samples were used for the quantification of pro‐inflammatory cytokines and vascular mediators. Levels of interleukin (IL)‐1beta, IL‐6, interferon (IFN)‐gamma and tumour necrosis factor (TNF)‐alpha were determined as part of a panel of 27 inflammatory markers, growth factors and chemokines measured simultaneously through a magnetic bead‐based immunoassay on a Bio‐Plex® System with Luminex xMAP® Technology (#M500KCAF0Y, Bio‐Rad Laboratories Inc.) as previously described.¹¹, ¹² Serum levels of adipsin, adiponectin and adhesion molecules [intercellular adhesion molecule‐1 (ICAM‐1) and vascular adhesion molecule‐1 (VCAM‐1)] were quantified on a Bio‐Plex® System according to the manufacturer's instructions (#171A7002M, Bio‐Rad). A commercially available ELISA kit was used to measure the levels of fibroblast growth factor 19 (FGF19) (DF1900, R&D system).

Ponziani F.R., Nesci A., Caputo C., Salvatore L., Picca A., Del Chierico F., Paroni Sterbini F., Marzetti E., Di Giorgio A., Santoro L., Putignani L., Gasbarrini A., Santoliquido A, & Pompili M. (2022). High prevalence of lower limb atherosclerosis is linked with the gut–liver axis in patients with primary biliary cholangitis. Liver International, 43(2), 370-380.

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Giardia Infection and FGF19 Levels

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Serum samples from 23 G. lamblia-positive and 60 G. lamblia-negative children, as determined by PCR from fecal samples, were obtained from the previously published study cohort (43) , stored at -80°, and analyzed by ELISA for FGF19 concentration (DF1900, R&D Systems).

Riba A., Hassani K., Walker A., van Best N., von Zezschwitz D., Anslinger T., Sillner N., Rosenhain S., Eibach D., Maiga-Ascofaré O., Rolle-Kampczyk U., Basic M., Binz A., Mocek S., Sodeik B., Bauerfeind R., Mohs A., Trautwein C., Kiessling F., May J., Klingenspor M., Gremse F., Schmitt-Kopplin P., Bleich A., Torow N., von Bergen M, & Hornef M.W. (2020). Disturbed gut microbiota and bile homeostasis in Giardia-infected mice contributes to metabolic dysregulation and growth impairment. Science translational medicine, 12(565).

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Quantification of Serum FGF-19 Levels

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Human serum concentration of FGF-19 was determined using the Human FGF-19 Quantikine enzyme-linked immunoassay (ELISA) (R&D Systems, DF1900). Assay was completed according to manufacturer’s instructions with samples run in duplicate.

Q. Bartlett A., Vesco K.K., Purnell J.Q., Francisco M., Goddard E., Guan X., DeBarber A., Leo M.C., Baetscher E., Rooney W., Naugler W., Guimaraes A.R., Catalano P., Xia Z, & Schedin P. (2021). Pregnancy and weaning regulate human maternal liver size and function. Proceedings of the National Academy of Sciences of the United States of America, 118(48), e2107269118.

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Serum FGF19 Quantification by ELISA

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Blood samples were processed within 1 h after collection and stored at −80 °C until analysis. Serum concentrations of FGF19 were evaluated using ELISA kits (R&D #DF1900) following the manufacturer’s instructions.

Li F., Li Z., Han Q., Cheng Y., Ji W., Yang Y., Lu S, & Xia W. (2020). Enhanced autocrine FGF19/FGFR4 signaling drives the progression of lung squamous cell carcinoma, which responds to mTOR inhibitor AZD2104. Oncogene, 39(17), 3507-3521.

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