L 2420

Manufactured by Waters Corporation

Sourced in Japan

The L-2420 is a high-performance liquid chromatography (HPLC) detector manufactured by Waters Corporation. It is designed to provide reliable and accurate detection of a wide range of analytes in liquid chromatography applications. The L-2420 utilizes a UV-Vis absorbance detection technology to identify and quantify the components of a sample as they elute from the HPLC column.

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Lab products found in correlation

High performance liquid chromatography (hplc), by Hitachi (3 mentions) L 2200, by Waters Corporation (3 mentions) Xselect hss c18, by Waters Corporation (2 mentions) At 330, by Waters Corporation (2 mentions) Waters c18 reversed phase column, by Waters Corporation (1 mentions) C18 reversed phase column, by Waters Corporation (1 mentions)

4 protocols using l 2420

Analytical Characterization of Reynoutria japonica

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A whole fresh plant was collected in August 2018 in the medicinal herb garden of School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, and was verified by associate professor Jia Lingyun as Reynoutria japonica Houtt. The plant was divided into several parts: leaves, stems, root phloem, root xylem and part of the tissues were dried at 60 °C until constant weight. The dried materials were grounded into fine powder and sealed in tube for content determination of polydatin, resveratrol and emodin using a Hitachi HPLC (Tokyo, Japan) system coupled with DAD detector (L-2420), auto-sampler (L-2200), column oven (AT-330), and reverse phase column (Waters, Milford, MA, USA, XSELECT™ HSS C₁₈, 4.6 × 250 mm, 5 µm).
Besides, plant material used for extraction was purchased from Guoda TCM pharmacy (Shanghai, China) and authorized by associate professor Jia Lingyun, as dried root and rhizome tissue of Reynoutria japonica Houtt. The plant materials were powdered to pass through 120 meshes and extracted to obtain RJE as described in Section 4.8.

Liu J., Zhang X., Yan T., Wang F., Li J., Jia L., Jia J, & Hu G. (2020). Screening of an Endophyte Transforming Polydatin to Resveratrol from Reynoutria Japonica Houtt and the Optimization of Its Transformation Parameters. Molecules, 25(20), 4830.

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Transdermal Ketoprofen Drug Delivery

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Drug-in-adhesive patch with 5% materials and 4% ketoprofen (KP) was prepared using solvent evaporation method. A drug release study was conducted with modified Franz diffusion cells at 32 °C. The patch was pasted on a cellulose membrane and then fixed onto the receptor cell with phosphate buffer solution (PBS, pH 7.4) of 4.5 mL. Samples of 2.0 mL were collected at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 26, 28, 30, 32, 34, 36, 48, 50, 52, 54, 56, 58, 60 and 72 h, and then replaced with the same volume of fresh PBS. The drug concentration was determined by Hitachi HPLC (Tokyo, Japan), which consisted of a pump L-2130, auto sampler L-2200 and UV-detector L-2420 and Waters C18 reversed-phase column (Waters, America) (200 × 4.6 mm, 5 μm). The mobile phase of KP was a mixture of methanol, water and acetic acid solution (60 : 40 : 0.2, v/v), and the pH was adjusted to 6.8 with triethylamine. The column temperature was 40 °C and the flow rate was 1 mL min⁻¹. The wavelength of the detector was set at 260 nm.
A simplified Higuchi equation was used to describe the drug release behavior as follows: where D and h are the diffusion rate and diffusion length, respectively. The release percent R was plotted against and k is the release rate.

Zhang Y., Sun C.Y, & Lin L. (2023). Coordination-directed self-assembly of nano-cages: metal ion-change, ligand-extending, shape-control and transdermal drug delivery. RSC Advances, 13(34), 23396-23401.

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HPLC Analysis of Echinacoside Quantification

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A Hitachi HPLC (Tokyo, Japan) system coupled with DAD detector (L-2420), auto-sampler (L-2200), column oven (AT-330), and reverse phase column (Waters, MA, USA, XSELECT™ HSS C₁₈, 4.6 × 250 mm, 5 µm) was applied for echinacoside content determination. The detection wavelength was set to be 330 nm according to the literature [23 ]. The elution condition was methanol-0.1% formic acid (30:70, v/v).
Standard compounds were accurately weighed and dissolved in 5 mL measuring flasks to final concentrations of 10.0 mg·mL⁻¹. Serial dilutions were made for standard curve preparation. 10 µL of each standard solution dilution was injected into HPLC. Regression analysis between echinacoside concentrations (X) and peak areas (Y) were conducted using a Microsoft 2007 Excel software. The standard curve and linear range of echinacoside was Y = 6 × 10⁶X − 4193.9, (R² = 0.9998, linear range: 0.0032 to 2.0 mg·mL⁻¹). Peak areas in samples with corresponding retention time was recorded and used to calculate the concentration in extract obtained from the Extract Preparation section.

Hu G., Wu T., Chang Y., Zhan X, & Jia J. (2018). Wound Stress, an Unheeded Factor for Echinacoside Accumulation in Cistanche deserticola Y. C. Ma. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(4), 893.

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HPLC Analysis of ATE Concentration

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The drug concentration was determined by Hitachi HPLC (Tokyo, Japan), which consisted of a Pump L-2130, AutoSampler L-2200, UV-detector L-2420, and C18 reversed-phase column (200 × 4.6 mm, 5 μm, ODS-2 Waters, Milford, MA). The mobile phase for the ATE was a mixture of methanol, water, and phosphoric acid solution (70:30:0.1, v/v). The column temperature was 25 °C, the flow rate was set at 0.7 mL/min, and the free drug was detected at a wavelength of 275 nm.

Zhang K., Zhuang Y., Zhang W., Guo Y, & Liu X. (2020). Functionalized MoS2-nanoparticles for transdermal drug delivery of atenolol. Drug Delivery, 27(1), 909-916.

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