24 well millicell hanging insert

Manufactured by Merck Group

The 24-well millicell hanging insert is a laboratory equipment product designed for cell culture applications. It features a permeable membrane that allows for the exchange of media and nutrients between the upper and lower chambers, facilitating the study of cellular processes and interactions. The product is offered in a 24-well format, providing a multifunctional platform for various experimental setups.

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Lab products found in correlation

24 well biocoat matrigel invasion chambers, by BD (2 mentions) Spot imaging software, by Nikon (2 mentions)

Close spelling variants of this product

Millicell inserts (70 citations) 24-well insert (16 citations) Millicell hanging inserts (12 citations) Millicells (10 citations) 24‐well Hanging Insert (2 citations) Millicell insert well (2 citations)

2 protocols using 24 well millicell hanging insert

Cell Migration and Invasion Assay

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Migration and invasion assays were performed in 24-well millicell hanging insert (Millipore) or 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences). In brief, 1 × 10⁵ cells were seeded to the top chamber and 10% FBS in medium was added to the bottom chamber as a chemoattractant. After 24 or 48-h incubation, the number of cells that invaded through the membrane (migration) or Matrigel (invasion) was counted in 10 fields and imaged using SPOT imaging software (Nikon).

Ming X.Y., Fu L., Zhang L.Y., Qin Y.R., Cao T.T., Chan K.W., Ma S., Xie D, & Guan X.Y. (2016). Integrin α7 is a functional cancer stem cell surface marker in oesophageal squamous cell carcinoma. Nature Communications, 7, 13568.

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Cell Migration and Invasion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Migration and invasion assays were performed in 24-well millicell hanging insert (Millipore) or 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences). In brief, 1×10⁵ cells were seeded to the top chamber and 10% FBS in medium was added to the bottom chamber as a chemoattractant. After 24 h or 48 h incubation, the number of cells that invaded through the membrane (migration) or Matrigel (invasion) was counted in 10 fields and imaged using SPOT imaging software (Nikon).

Ming X.Y., Zhang X., Cao T.T., Zhang L.Y., Qi J.L., Kam N.W., Tang X.M., Cui Y.Z., Zhang B.Z., Li Y., Qin Y.R, & Guan X.Y. (2018). RHCG Suppresses Tumorigenicity and Metastasis in Esophageal Squamous Cell Carcinoma via Inhibiting NF-κB Signaling and MMP1 Expression. Theranostics, 8(1), 185-198.

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