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3 protocols using sodium dodecyl sulfate (sds)

1

Synthesis and Characterization of Phenolphthalin

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Potassium 2,5-dihydroxybenzenesulfonate (SHQ), 2,6-difluorobenzonitrile (DFBN), deuterated dimethyl sulfoxide (DMSO-d6) and N,N-dimethyl formamide (DMF) were obtained from Sigma Aldrich (Shanghai, China). Bisphenol A (BPA), phenolphthalein (PP), zinc (Zn), sodium hydroxide (NaOH), potassium carbonate (K2CO3), N-methyl pyrrolidone (NMP), toluene, ethanol, tetrahydrofuran (THF), N,N-dimethylformamide (DMF), dichloromethane (CH2Cl2) and sodium dodecyl sulfate (SDS) were received from Chengdu Kelong Chemical Co. (Chengdu, China). Methylene blue (MB) and methyl orange (MO) were purchased from Sinopharm chemical reagent (Shanghai, China). Phenolphthalin (PPL) was synthesized from phenolphthalein (PP), Zn and NaOH.
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2

Insecticide and Synergist Evaluation

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Five different insecticides were used in this research, including 74% emamectin benzoate (Nanjing Red Sun Co., Ltd., Gaochun, China), 97% chlorpyrifos (Hubei Sharonda Co., Ltd., Jingzhou, China), 95% chlorantraniliprole (Corteva Agriscience, Indianapolis, United States), 95% indoxacarb (Jiangsu Flag Chemical Industry Co., Ltd., Nanjing, China), and 99% spinetoram (Wuhan Xiyu Biotechnology Co., Ltd., Hubei, China). The synergists included diethyl maleate (DEM), piperonyl butoxide (PBO), and triphenyl phosphate (TPP), which were bought from Aladdin Shanghai Co., Ltd. (Nanjing, China). α-Naphthyl acetate (α-NA), 1-chloro-2,4-dinitrobenzene (CDNB), and fast blue salt B were purchased from Chengdu Ai Keda Chemical Technology Co., Ltd. (Chengdu, China). Bovine serum albumin (BSA), NADPH, DTT, PMSF, and reduced glutathione (GSH) were purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). The Coomassie brilliant blue G 250, EDTA Na2, and sodium dodecyl sulfate (SDS) were bought from Chengdu Kelong Chemical Reagent Co., Ltd. (Chengdu, China).
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3

Human Hair Extraction and Analysis

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Materials: Human hair was collected from local hair salons in Chongqing, China.
Sodium dodecyl sulfate (SDS), thioglycolic acid (TGA), hydrochloric acid and sodium hydroxide were purchased from Kelong Chemical reagent Co., Ltd. (Chengdu, China).
Amicon ultra-filtration centrifuge tubes (10, 30, 50, and 100 kDa) were purchased from Millipore Corporation (Bedford, USA). Enzyme-linked immunosorbent assay (ELISA) kits were purchased from the Neobioscience Technology Company (Beijing, China). Other reagents used in this study were of analytical grade.
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