All CT scans including non-contrast scan and contrast-enhanced triple phases were performed with the Siemens
SOMATOM Sensation 64 CT scanner (Erlangen, Germany) within 7 days before the initial TACE treatment. For standardized analysis of each patient, a cross-sectional enhanced CT images at the third lumbar vertebra (L3) was selected. The areas of skeletal muscle area (SM) and adipose tissue [subcutaneous, (SAT); visceral (VAT)] were measured by using the
Slice-O-Matic software (version 5.0; Tomovision, Montreal, Canada), and the calculation was based on the Hounsfield units (HU) thresholds (−29 to 150 HU for SM, −190 to −30 for SAT and −150 to −50 for VAT) (von Hessen et al., 2021 (
link)). All of the three variables were normalized for the height in m
2 and expressed as indexes (cm
2/m
2). The skeletal muscles at L3 consisted of the psoas major, the erector spinae, the quadratus lumborum, the rectus abdominis, the transversus abdominis, the internal oblique, and the external oblique. Additionally, the density of each variable was calculated in HU. For the measurements of the variables, two observers with more than 5 years of experience in abdominal radiology independently measured the CT images from 30 randomly selected patients and compared the results, and the inter-observer agreement was 97.0%.
Li Q., Zhang L., Hou Z.H., Zhao D.X., Li J.B., Zhang S., Yin Y., Ni C.F, & Chen T. (2021). High Visceral Adipose Tissue Density Correlates With Unfavorable Outcomes in Patients With Intermediate-Stage Hepatocellular Carcinoma Undergoing Transarterial Chemoembolization. Frontiers in Cell and Developmental Biology, 9, 710104.