Anti RhoA, Cdc42, Rac1, and β-tubulin antibodies were purchased from Cell Signaling Technology (USA). ST1937 and SB258585 were obtained from Tocris (Bristol, UK). C3 transferase was from Cytoskeleton Inc. (USA). Y-27632 was a product of Invitrogen (USA). PP2, H89, and PD98059 were products of Calbiochem (USA). 5-HT and other chemicals were purchased from Sigma (USA). Protease inhibitor mixture were from BioVision (USA).
Sb258585
Manufactured by Bio-Techne
Sourced in United Kingdom
SB258585 is a small molecule inhibitor that selectively blocks the activity of the p38 mitogen-activated protein kinase (MAPK) signaling pathway. It is used as a research tool to study the biological functions of the p38 MAPK cascade.
Automatically generated - may contain errors
Lab products found in correlation
St1936, by Bio-Techne (2 mentions)
As 19, by Bio-Techne (2 mentions)
Anti rhoa, by Cell Signaling Technology (1 mentions)
C3 transferase, by Cytoskeleton (1 mentions)
Y27632, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor mixture, by Abcam (1 mentions)
Cdc42, by Cell Signaling Technology (1 mentions)
β tubulin, by Cell Signaling Technology (1 mentions)
Cisapride, by Bio-Techne (1 mentions)
5 ht hydrochloride, by Bio-Techne (1 mentions)
Anti tuj1, by BioLegend (1 mentions)
Anti cdk5, by Santa Cruz Biotechnology (1 mentions)
Sb206553, by Merck Group (1 mentions)
Roscovitine, by Selleck Chemicals (1 mentions)
Trimipramine maleate, by Merck Group (1 mentions)
Amitriptyline hydrochloride, by Bio-Techne (1 mentions)
Sc 17320, by Santa Cruz Biotechnology (1 mentions)
Anti actin, by Merck Group (1 mentions)
Anti app ctf, by Merck Group (1 mentions)
Sb271046, by Selleck Chemicals (1 mentions)
5 protocols using sb258585
1
Reagents and inhibitors for RhoA, Cdc42, Rac1
2
Skeletal Staining of Newborn Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice received intraperitoneal injection of ST1936 (10 mg/kg, Tocris Bioscience) or SB258585 (5 mg/kg, Tocris Bioscience) four times at E6, E9, E12, E15. Briefly, newborn mice were deskinned, eviscerated, and immersed in 100% ethanol for 24 h. The samples were fixed in acetone for 24 h and then stained for 24 h in a solution containing 0.1% Alizarin Red, 0.3% Alcian Blue, acetic acid, and 70% ethanol (1:1:1:17, v/v/v/v). They were then transferred to a solution of 1% KOH in 20% glycerol until clear and then stored in glycerol.
3
Serotonin Receptor Ligands and Rho GTPase Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following 5-HTR ligands and Rho GTPase family inhibitors were purchased from Tocris Bioscience (Bristol, UK): 8-hydroxy-PIPAT oxalate, alpha-methyl-5-hydroxyltryptamine maleate, N-methylquipazine dimaleate, cisapride, 5-carboxamidotrypamine maleate, ST1936 oxalate, AS 19, SB258585, 5-HT hydrochloride. rhosin hydrochloride, NSC 23766, and ZCL 278.
4
Pharmacological Modulation of BACE1 and APP Processing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluoxetine, TAPI-1, SB271046, SB742457, L-685,458 and roscovitine were purchased from Selleck Chemicals. Amitriptyline hydrochloride, ST1936 and SB258585 were from Tocris Bioscience. Protriptyline hydrochloride, amoxapine, trimipramine maleate, SB215505, SB206553, cAMP, L-ascorbic acid and DAPI were purchased from Sigma. Escitalopram oxalate was purchased from Lundbeck. Sertraline hydrochloride was from Pfizer. BACE inhibitor IV (BSI IV) was from Calbiochem. Recombinant human BDNF, GNDF, and IGF-I were from Peprotech. CellTiter-Glo was from Promega. Immunoblotting was performed with the following antibodies: anti-ADAM10 (Ab1997, Abcam), anti-BACE1 N-termimus (AP7774b, Abgent), anti-APP-CTF (A8717, Sigma), anti-actin (A2066, Sigma), anti-CDK5 (sc-173, Santa Cruz), and Rabbit anti-β-arrestin-1/2 (A1CT) antibody was a kind gift from Dr. Robert J. Lefkowitz. Immunofluorescence staining was performed with the following primary antibodies: anti-Tuj1 (801201, BioLegend), anti-Sox2 (sc-17320, Santa Cruz), anti-Map2 (Ab5622, Millipore), and anti-GFAP (sc-6170, Santa Cruz).
5
Pharmacological modulation of neural activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AS19 (#1968), SB242084 (2901/10), SB269970 (#1612/10), SB258585 (#1961/10), WAY100635 (#4380), 8-OH-DPAT (#0529/10), WAY629 (#2173), 5-Carboxamidotryptamine meleate (5-CT, #0458),
D-2-amino-5-phosphonovalerate (AP5, #0106), bicuculline methiodide (Bic, #2503), and 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX, #0190) were purchased from Tocris Bioscience. Serotonin hydrochloride (5-HT, #H9523) was obtained from Sigma–Aldrich. All drugs were dissolved in water or DMSO and aliquoted as stock solutions that were stored at −80 °C. On the day of recording, the stock solutions were diluted to final concentrations in ACSF (at least 1:1000 dilution) for the experimental test. For experiments when DMSO was used for preparing stock solutions of some drugs (AS19, SB242084, and CNQX), we also added 0.1% of DMSO in ACSF of control condition. During the recording, drugs were delivered locally via a multi-channel drug application system (Warner Instruments, CT, USA) with a flow pipette of 250-μM diameter so that the drug solutions were quickly washed out after the drug channel was switched to normal ACSF.
D-2-amino-5-phosphonovalerate (AP5, #0106), bicuculline methiodide (Bic, #2503), and 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX, #0190) were purchased from Tocris Bioscience. Serotonin hydrochloride (5-HT, #H9523) was obtained from Sigma–Aldrich. All drugs were dissolved in water or DMSO and aliquoted as stock solutions that were stored at −80 °C. On the day of recording, the stock solutions were diluted to final concentrations in ACSF (at least 1:1000 dilution) for the experimental test. For experiments when DMSO was used for preparing stock solutions of some drugs (AS19, SB242084, and CNQX), we also added 0.1% of DMSO in ACSF of control condition. During the recording, drugs were delivered locally via a multi-channel drug application system (Warner Instruments, CT, USA) with a flow pipette of 250-μM diameter so that the drug solutions were quickly washed out after the drug channel was switched to normal ACSF.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!