Butylhydroxytoluene bht

Most native and deuterated standards for analysis of oxylipins, endocannabinoids, and endocannabinoid-related lipids, including 12-[[(cyclohexylamino)carbonyl]amino]-dodecanoic acid (CUDA) used for recovery calculation purposes, were obtained from Cayman Chemical (Ann Arbor, MI, USA). Only 9,10,13-trihydroxyoctadecenoic acid (TriHOME) and 9,12,13-TriHOME were obtained from Larodan (Malmö, Sweden). Acetonitrile (ACN) and methanol (MeOH) were from Merck (Darmstadt, Germany). Isopropanol was from VWR PROLABO (Fontenay-sous-Bois, France). Acetic acid was purchased from Aldrich Chemical Company, Inc. (Milwaukee, WI, USA). Butylhydroxytoluene (BHT) was from Cayman Chemical (Ann Arbor, MI, USA) and ethylenediaminetetraacetic acid (EDTA) from Fluka Analytical, Sigma-Aldrich (Buchs, Switzerland). Glycerol was from Fischer Scientific (Loughborough, UK). All solvents and chemicals were of HPLC grade or higher and ultrapure water was used (Milli-Q Gradient system, Millipore, Milford, MA, USA).

The following native and deuterated standards were purchased from Cayman Chemicals (Ann Arbor, MI, USA): 2-AG, AEA, PEA, SEA, OEA, LEA, POEA, EPEA, DEA, NA-Gly, 2-AG-d₈, AEA-d₈, PEA-d₄, SEA-d₃, OEA-d₄, 12-[[(cyclohexylamino)carbonyl]amino]-dodecanoic acid (CUDA). Acetonitrile and methanol were from Merck (Darmstadt, Germany). Isopropanol was from VWR PROLABO (Fontenay-sous-Bois, France). Acetic acid was purchased from Aldrich Chemical Company, Inc. (Milwaukee, WI, USA). Butylhydroxytoluene (BHT) was from Cayman Chemical (Ann Arbor, MI, USA) and ethylenediaminetetraacetic acid (EDTA) from Fluka Analytical, Sigma-Aldrich (Buchs, Switzerland). Glycerol was from Fischer Scientific (Loughborough, UK). All solvents and chemicals were of HPLC grade or higher. Water was purified by a Milli-Q Gradient system (Millipore, Milford, MA, USA).