Mda mb 361 cells

Human HER2-amplified breast cancer BT474 cells (ATCC) were cultured in RPMI 1640 supplemented with 10% FBS (Atlanta Biologicals, Flowery Branch, GA, USA). Human HER2-amplified MDA-MB-361 cells (ATCC) were cultured in DMEM/F12 supplemented with 10% FBS. BT474 and MDA-MB-361 cells were transduced with an expression cassette encoding Gaussia luciferase (Gluc) and green fluorescent protein (GFP), as previously described.⁶

MCF7, MDAMB231, T47D, MCF10A, Hs578T and HEK293 cell lines were obtained from ATCC and grown according to their guidelines. MDAMB361 cells (ATCC) were grown in DMEM (Gibco Invitrogen) with 20% fetal bovine serum (FBS; Gibco Invitrogen) and 1% antibiotics (Gibco Invitrogen). B80T5 cells (a gift from Roger Reddel; CMRI, Australia) were grown in RPMI (Gibco Invitrogen) with 10% FBS and 1% antibiotics. K5+/K19 + cells [12 (link)] were grown in 1:1 MEM α (Gibco Invitrogen) and Ham’s F-12 Nutrient Mix (Gibco Invitrogen) with 1% FBS, 10mM HEPES, 1 µg/ml bovine pancreatic insulin, 1 µg/ml hydrocortisone, 50 µg/ml epidermal growth factor (Sigma Aldrich), 10 mg/ml transferrin, 100 µM β-estradiol, 2 mM glutamine, 2.6 ng/ml sodium selenite, 1 ng/ml cholera toxin (Sigma Aldrich), 6.5 ng/ml triiodothyronine, 100 µM ethanolamine, 35 µg/ml BPE, 10 µg/ml gentamicin, 10 µg/ml ascorbic acid, 15 µg/ml hygromycin B. All cell lines were tested for mycoplasma contamination and verified by short tandem repeats (STR) profiling.