Supelco discovery bio wide pore c18 column

Manufactured by Merck Group

Sourced in United States

The Supelco Discovery BIO Wide Pore C18 column is a reverse-phase high-performance liquid chromatography (HPLC) column designed for the separation and analysis of large biomolecules. The column features a wide pore size that enables the effective separation of proteins, peptides, and other macromolecules. It is composed of high-purity silica particles with a chemically bonded C18 stationary phase, providing optimal chromatographic performance for the targeted application.

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Lab products found in correlation

Ezprep, by Teledyne (2 mentions) Combiflash, by Teledyne (2 mentions) Microotof qiii, by Bruker (1 mentions) Dionex ultimate 3000 lc, by Thermo Fisher Scientific (1 mentions) Recombinant human insulin, by Merck Group (1 mentions) Milli q plus system, by Merck Group (1 mentions) Tx 100, by Merck Group (1 mentions) Na2hpo4 12h2o, by Merck Group (1 mentions) Nah2po4 2h2o, by Merck Group (1 mentions) Microtof q 3 spectrometer, by Bruker (1 mentions) Congo red, by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Thioflavin t, by Merck Group (1 mentions)

2 protocols using supelco discovery bio wide pore c18 column

Synthesis and Characterization of Collagen Peptides

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Model collagen fragments H-Gly-Hyp-Pro-Ala-Hyp-Pro-OH (1), H-(Pro)₆-OH (2), H-(Pro)₉-OH (3), H-(Hyp)₆-OH (4) and H-(Hyp)₉-OH (5) were synthesized on chlorotrityl resin according the Fmoc/tBu strategy.
Analytical RP-HPLC was performed on a LC Dionex UltiMate 3000 (ThermoFisher Scientific, Waltham, MA, USA), using a Kinetex Reversed Phase C18 column (100 × 4.6 mm). Gradients of 0.1% TFA in H₂O (B) and 0.1% TFA in CH₃CN (A) were used, at a flow rate 0.4 mL/min with UV detection at 220 and 254 nm. Preparative HPLC was done on a CombiFlash, EZPrep, Teledyne ISCO (Lincoln, NE, USA) using a Supelco Discovery BIO Wide Pore C18 column (25 cm × 21.2 mm, 10 mm; Sigma-Aldrich) flow rate 5 mL/min, detection wavelengths 220 and 254 nm, gradient ratio A (0.1% TFA in CH₃CN) and B (0.1% TFA in H₂O) 0:100 to 18:82 in 30 min, followed by an isocratic run for 5 min.
Mass Spectrometry analysis was performed on a Bruker microOTOF-QIII (Bruker Corporation, Billerica, MA, USA).

Wasko J., Fraczyk J., Becht A., Kaminski Z.J., Flinčec Grgac S., Tarbuk A., Kaminska M., Dudek M., Gliscinska E., Draczynski Z, & Kolesinska B. (2020). Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine. Materials, 13(14), 3079.

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Insulin Aggregation Characterization

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Human recombinant insulin (Sigma-Aldrich, Poznan, Poland), essentially fatty acid free human serum albumin (HSA) (Sigma-Aldrich), 4-(1-pyrenyl)butyric acid (Merck, Warsaw, Poland), Human recombinant insulin (Sigma-Aldrich), Congo Red, Thioflavin T (Sigma-Aldrich, Poznan, Poland), and all necessary amino acid derivatives (Merck) were used as received. NaH₂PO₄∙2H₂O, Na₂HPO₄∙12H₂O, TX-100, EtOH, MeOH, and NaCl (Sigma-Aldrich) were used for the buffer preparation. Water was purified with the use of a Millipore Milli-Q Plus system.
Analytical HPLC: UltiMate 3000 UHPLC System Thermo Scientific™; column parameters: Kinetex 2.6 u C18 100A, 100 × 4.6 mm, 20 °C; diode array UV/Vis detector (DAD); eluent ACN/H₂O; gradient 0–2 min 3/97, 2–31 min 95/5, 31–32 min 0/100, 32–33 min 0/100, 33–35 min 3/97, 35–37.5 min 3/97.
Preparative HPLC: CombiFlash, EZPrep, Teledyne ISCO, Supelco Discovery BIO Wide Pore C18 column (25 cm × 21.2 mm, 10 mm; Sigma Aldrich); flow rate, 5 mL/min; detection wavelengths, 220 and 254 nm); gradient ratio A (0.1% TFA in ACN)/ B (0.1% TFA in H₂O) 0:100 to 18:82 in 30 min, followed by an isocratic run for 5 min.
ESI/MS: micrOTOF-Q III spectrometer Bruker Daltonics equipped with electrospray source (ESI) and time of flight detector (TOF).

Wasko J., Wolszczak M., Kaminski Z.J., Steblecka M, & Kolesinska B. (2020). Human Serum Albumin Binds Native Insulin and Aggregable Insulin Fragments and Inhibits Their Aggregation. Biomolecules, 10(10), 1366.

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