Nis elements d v 3

Microscopic preparations were mounted in water, 3 % potassium hydroxide (KOH) or lactic acid (LA). Methods of microscopy included stereomicroscopy using a Nikon SMZ 1500 and Nomarski differential interference contrast (DIC) using the Zeiss Axio Imager.A1 compound microscope. Images and data were gathered using the Nikon DS-U2 or Zeiss Axiocam 506 color digital cameras and measured by using the NIS-Elements D v.3.0 or Zeiss ZEN Blue Edition softwares. For certain images of ascomata the stacking software Zerene Stacker v.1.04 (Zerene Systems, Richland, WA, USA) was used. Measurements are reported as maxima and minima in parentheses and the range representing the mean plus and minus the standard deviation of a number of measurements given in parentheses.

Cultures were prepared and maintained as described previously (Jaklitsch 2009 (link)). Microscopic observations were made in tap water, except where noted. Morphological analyses of microscopic characters were carried out as described earlier (Jaklitsch 2009 (link)). Methods of microscopy included stereomicroscopy using Nikon SMZ1500, Olympus SZX10 and Euromex Novex RZ 65.560, light microscopy using Euromex XHR MIC 625, Olympus BX51 and Nomarski differential interference contrast (DIC) using the compound microscopes Nikon Eclipse E600 and Zeiss Axio Imager.A1. Images and data were gathered with Nikon Coolpix 4500, Nikon DS-U2, Nikon D90, Olympus DP72 and Zeiss Axiocam 506 colour digital cameras and measured directly with the microscope, or with Olympus cellSens Dimension, NIS-Elements D v.3.0 and Zeiss ZEN Blue Edition softwares. Amyloidity of asci was assessed using Lugol or Melzer reagent. Measurements are reported as maximum and minimum in parentheses and the range representing the mean plus and minus the standard deviation of a number of measurements given in parentheses.