Pretreated HCC cells were stained with an Annexin V-fluorescein isothiocyanate/PI apoptosis detection kit (BD Biosciences, La Jolla, CA, USA) according to the manufacturer's instructions. Stained cells were analyzed by FACSCalibur.
Annexin 5 fluorescein isothiocyanate pi apoptosis detection kit
Manufactured by BD
Sourced in United States
The Annexin V-fluorescein isothiocyanate/PI apoptosis detection kit is a laboratory equipment designed to detect and quantify apoptosis, a type of programmed cell death, in cellular samples. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a DNA-binding dye, to identify cells undergoing different stages of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Facscalibur flow cytometer, by BD (1 mentions)
Novocyte flow cytometry, by Agilent Technologies (1 mentions)
P akt, by Santa Cruz Biotechnology (1 mentions)
P pi3k, by Santa Cruz Biotechnology (1 mentions)
Hdac2, by Santa Cruz Biotechnology (1 mentions)
Ly294002, by Selleck Chemicals (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
5 protocols using annexin 5 fluorescein isothiocyanate pi apoptosis detection kit
1
Annexin V-FITC/PI Apoptosis Assay
2
Apoptosis Profiling of Breast Cancer Cells
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Cell apoptosis was assessed by flow cytometry using an Annexin-V-fluorescein isothiocyanate/PI apoptosis detection kit (BD Biosciences, San Jose, CA, USA), according to the manufacturer's instructions. The pretreated MCF-7 and MDA-MB-231 cells were harvested and washed twice with PBS (4°C). Following this, they were resuspended in 1X binding buffer at a concentration of 1×106 cells/ml, stained with Annexin V/PI and kept on ice for 30 min in the dark. The cells were then analyzed on a FACSCalibur™ flow cytometer (BD Biosciences). Assays were performed three times in triplicate.
3
Measuring Apoptosis in Transfected Cell Lines
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Following incubation of transfected TPC-1 and FTC133 cells for 48 h, the induction of apoptosis was measured using the Annexin V-fluorescein isothiocyanate/PI apoptosis detection kit (BD Biosciences), according to the manufacturer's instructions. Flow cytometry was performed to detect cell apoptosis (early apoptotic cells + late apoptotic cells; upper right quadrant + lower right quadrant) and the results were analyzed using Kaluza Analysis (version 2.1.1.20653; Beckman Coulter, Inc.).
4
Apoptosis Analysis of siRNA-Transfected Cells
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The siRNA-transfected cells were treated with 5 ug/mL of 5-Fu for 48 hours before harvest. For the analysis, cells were stained with the annexin V-fluorescein isothiocyanate/PI Apoptosis Detection Kit (BD Pharmingen), following the manufacturer’s instructions. Stained cells were analyzed by NovoCyte flow cytometry (ACEA Biosciences). Three independent experiments were performed.
5
Selective HDAC2 Inhibitor Induces Apoptosis
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Novel selective HDAC2 inhibitor CAY10683, IM (STI571) and LY294002 were purchased from Selleck Chemicals (USA). Annexin V-fluorescein isothiocyanate/PI apoptosis detection kit was obtained from BD Biosciences (BD Biosciences, San Jose, CA, USA). Antibodies specific for C-caspases3, C-poly (ADP-ribose) polymerase (PARP), P21, CDK1, β-actin, HDAC2, P-PI3K, PI3K, P-AKT, AKT were bought from Santa Cruz Biotechnology (Santa Cruz, CA). Secondary antibodies (HRP-conjugated goat anti-rabbit or anti-mouse) were also purchased from Santa Cruz Biotechnology (Santa Cruz, CA).
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