Day 1 adult worms were mounted on 2% agarose pads with 1 mm sodium azide as anesthetic and imaged with a Zeiss laser-scanning microscope (LSM710) using Zen (black edition) acquisition software (Carl Zeiss, Oberkochen, Germany). GFP fluorescence and phase contrast images were collected using a 488 laser, and mCherry fluorescence was collected using a 561 laser. At ×20, slices were taken every 1.7 μ using a 1.7-μ pinhole. At ×63 (water immersion objective), slices were taken every 0.8 μ using a 0.9-μ pinhole.
Zen black edition acquisition software
Manufactured by Zeiss
Sourced in Germany
The Zen (black edition) acquisition software is a core component of Zeiss' imaging solutions. It provides a platform for controlling and managing the data acquisition process of various microscopy systems. The software enables users to capture, process, and manage high-quality images and data from Zeiss microscopes and related imaging equipment.
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Lab products found in correlation
3 protocols using zen black edition acquisition software
1
Microscopic Imaging of C. elegans
2
3D Imaging of Fluorescent Neurons
3
Invadopodia Localization Assay
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The 2.5D invadopodia localization assay was adapted from Magalhaes et al. (2011) (link). In brief, growth factor–reduced Matrigel (2.5 mg/ml; BD) was mixed with 0.2 mg/ml Alexa Fluor 405 gelatin (1:20 dilution) and applied to the top chamber of a 1.0-µm transwell support (Corning). Excess Matrigel/gelatin solution was removed, and both chambers were allowed to equilibrate in plain DMEM at 37°C for 1 h. After equilibration, the bottom chamber was filled with 750 µl of DMEM/10% FBS. 30,000 cells were resuspended in 200 µl of DMEM/0.5% FBS, plated in the top chamber, and allowed to invade for 24 h. Cells were fixed in 3.7% paraformaldehyde and immunofluorescently labeled for cortactin and pY402-Pyk2. Membranes were imaged using an inverted laser-scanning confocal microscope (LSM780; 60× 1.4 NA with oil objective with ZEN black edition acquisition software; ZEISS). 3D invadopodia images were reconstructed using Imaris 8.0.0 (Bitplane).
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