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8 protocols using c16h18cln3s

1

Synthesis and Characterization of Metal-Organic Frameworks

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All materials utilized in this investigation were of analytical quality. C8H6O4 (benzene-1,4-dicarboxylic acid (H2BDC), 99%) was supplied by Sigma-Aldrich. C3H7NO (N,N-dimethylformamide (DMF), 99%), ZrCl4 (zirconium tetrachloride, 99%), CH3OH (methanol, 99%), C14H14N3NaO3S (methyl orange, 99%), C16H18ClN3S (methylene blue, 99%), and C32H22N6Na2O6S2 (Congo red, 99%) were purchased from Merck (see Table 1). In addition, demineralized water was purchased from a local market.
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2

Melamine-based Photocatalytic Materials

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The materials used in this work are melamine (C3N3(NH2)3, Sigma-Aldrich), citric acid monohydrate (C6H8O7·H2O, Merck, ACS grade), methylene blue (C16H18ClN3S, Merck), sodium hydroxide (NaOH, Merck), acetic acid (CH3COOH, Merck), ethylene glycol ((CH2OH)2, Merck), ZSM-5 (Pingxiang Naike Chemical), dimethyl sulfoxide (DMSO, (CH3)2SO, Sigma-Aldrich), methanol (CH3OH, SAP chemicals), isopropyl alcohol (IPA, (CH3)2CHOH, SAP chemicals) and l-ascorbic acid (C6H8O6, SAP chemicals) and deionized water.
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3

Serpentine-based Dye Adsorption Study

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Herein, serpentine obtained from Wadi Ghadir, Eastern desert in Egypt was subjected to grinding and sieving to get powder with fraction size <100 mesh. The chemicals used were: methylene blue (MB, C16H18ClN3S, Sigma–Aldrich, CAS 122965-43-9, and purity 98.5%), malachite green (MG, C23H25N2, Sigma-Aldrich CAS 123333-61-9, and purity 99%), hydrogen peroxide (30%, H2O2, Merck, Germany, CAS 7722-84-1), ferric chloride (FeCl3·6H2O, Loba Chemie, Mumbai, India, CAS 10025-77-1, purity 97%), iron (II) sulfate (FeSO4.7H2O, Loba Chemie, Mumbai, India, CAS 7782-63-0, purity 99%), ammonia hydroxide solution (NH4OH, Loba Chemie, CAS 1336-21-6, purity 25%), and distilled water. To obtain the required pH value, HCl (0.01 M) and NaOH (0.01 M) were used. The molecular structures of studied dyes (MG and MB) are shown in Figure 1.
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4

Zeolite-Mediated Dye Adsorption and Metal Ion Removal

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Zeolite mordenite, sodium (SiO2:Al2O3 mole ratio 13:1, Alfa Aesar), Methylene blue (MB), (C16H18ClN3S, Sigma-Aldrich), Silver nitrate (AgNO3, Sigma-Aldrich, 99.98%), Copper (II) nitrate. (Cu(NO3)2.2.5H2O, 98+%, Chem-Lab NV), Sodium hydroxide (NaOH, Sigma-Aldrich, ≥ 98% ).
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5

Chitosan-based Dye Removal Protocol

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Chitosan (medium molecular weight, 75–85% deacetylated), sodium persulfate (PS, Na2S2O8, >98%), and methylene blue (C16H18ClN3S, ≥95%) were obtained from Sigma-Aldrich. Glacial acetic acid (CH3COOH, 100%, EMSURE® ACS), hydrochloric acid (HCl, 37%, EMSURE® ACS), and sodium hydroxide (NaOH, 99–100%, EMSURE® ACS) were obtained from Merck Millipore.
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6

Synthesis of Photocatalyst Compounds

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The following chemicals were used for the synthesis of photocatalysts, bismuth nitrate pentahydrate (Bi(NO3)3·5H2O, Sigma Aldrich 98%), ammonium metavanadate (NH4VO3, Sigma Aldrich 98%), cobalt(ii) nitrate hexahydrate (Co(NO3)2·6H2O, Sigma Aldrich 98%), sodium hydroxide (NaOH, Sigma Aldrich 98%) and sodium borohydride (NaBH4, Sigma Aldrich 98%). To monitor the degradation activities, methylene blue (MB) dye (C16H18ClN3S, Sigma Aldrich), Congo red (CR) dye (C32H22N6Na2O6S2), and rhodamine B (C28H31ClN2O3, Sigma Aldrich) were used.
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7

Zinc Chloride Synthesis Protocol

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Zinc chloride (ZnCl2), sodium hydroxide (NaOH), ethanol (C2H5OH) and methylene blue (MB) dye with the chemical formula C16H18ClN3S were received from Sigma-Aldrich (Prague, Czech Republic). The chemicals were used as received.
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8

Zebrafish Embryo Cultivation and Disinfection

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Zebrafish (Danio rerio) embryos were obtained from fish facilities at the University of Malaga following standard procedures (Kimmel et al., 1995; (link)Truong et al., 2011) (link).
A breeding stock of adult zebrafish was kept on a recirculating system at 27 ± 1°C. Fishes were maintained with a standard 12:12-hour light-dark photoperiod. Embryos were produced by one-to-one or one-to-two female-male mating in fish hatch box. Fertilized eggs are collected and disinfected using a 0.5% bleach solution during 1 min and washed several times with E3 embryo media (5.00 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2, 0.33 mM MgSO4, 0.00003% C16H18ClN3S; Sigma-Aldrich). Clean eggs were incubated in E3 medium in a Petri dish at 28 ± 0.1 ᵒC.
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