Log In Sign up
The largest database of trusted experimental protocols

Texas red labeled secondary antibody

Manufactured by Santa Cruz Biotechnology
Visit Supplier

The Texas Red-labeled secondary antibody is a fluorescent detection reagent used in various biomedical applications. It is designed to bind to primary antibodies and emit a red fluorescent signal, allowing for the visualization and detection of target proteins or cells in a sample.

Automatically generated - may contain errors

Lab products found in correlation

β catenin antibody, by Santa Cruz Biotechnology (2 mentions) Cd133, by Santa Cruz Biotechnology (1 mentions) Cd117 c kit, by Santa Cruz Biotechnology (1 mentions) Cd105 endoglin, by Santa Cruz Biotechnology (1 mentions) Cd166 alcam, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Texas Red

4 protocols using texas red labeled secondary antibody

1

Immunofluorescent Visualization of β-Catenin

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining was performed as described [12 (link), 33 (link), 42 (link), 49 , 54 (link), 55 (link)]. Briefly, cells were infected with AdWnt3A or AdGFP for 48h, fixed with methanol, permeabilized with 1% NP-40, and blocked with 10% BSA, followed by incubating with β-catenin antibody (Santa Cruz Biotechnology). After being washed, cells were incubated with Texas Red-labeled secondary antibody (Santa Cruz Biotechnology). Stains were examined under a fluorescence microscope. Stains without primary antibodies, or with control IgG, were used as negative controls.
Zhang H., Wang J., Deng F., Huang E., Yan Z., Wang Z., Deng Y., Zhang Q., Zhang Z., Ye J., Qiao M., Li R., Wang J., Wei Q., Zhou G., Luu H.H., Haydon R.C., He T.C, & Deng F. (2014). Canonical Wnt signaling acts synergistically on BMP9-induced osteo/odontoblastic differentiation of stem cells of dental apical papilla (SCAPs). Biomaterials, 39, 145-154.
+ Open protocol
+ Expand
2

Immunofluorescence Assay for Smad Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence assay was carried out as described 21 (link), 72 (link), 73 (link). Briefly, subconfluent cells were stimulated with BMP9-cm or Con-cm, for the indicated time the cells were fixed with methanol, permeabilized with 1% NP-40, and blocked with 10% donkey serum, followed by incubating with p-Smad1/5/8 antibody (Santa Cruz Biotechnology). After being washed, cells were incubated with a Texas Red-labeled secondary antibody (Santa Cruz Biotechnology). Stains were examined under a fluorescence microscope. Stains without primary antibodies, or with control IgG, were used as negative controls.
Li R., Yan Z., Ye J., Huang H., Wang Z., Wei Q., Wang J., Zhao L., Lu S., Wang X., Tang S., Fan J., Zhang F., Zou Y., Song D., Liao J., Lu M., Liu F., Shi L.L., Athiviraham A., Lee M.J., He T.C, & Zhang Z. (2016). The Prodomain-Containing BMP9 Produced from a Stable Line Effectively Regulates the Differentiation of Mesenchymal Stem Cells. International Journal of Medical Sciences, 13(1), 8-18.
+ Open protocol
+ Expand
3

Immunofluorescence Staining of Stem Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining was performed as described [22] (link), [24] (link), [35] (link), [37] (link), [40] (link), [52] . Briefly, cells were fixed with methanol, permeabilized with 1% NP-40, and blocked with 10% BSA, followed by incubating with CD73, CD44, CD90, CD117/c-kit, CD29, CD133, CD105/endoglin, CD166/ALCAM, or BMPR-II antibody (Santa Cruz Biotechnology) for 1 hr at room temperature. After being washed, cells were incubated with Texas Red-labeled secondary antibody (Santa Cruz Biotechnology) for 30 min. Cell nuclei were stained with DAPI. Stains were examined under a fluorescence microscope. Stains without primary antibodies, or with control IgG, were used as negative controls.
Wang N., Zhang W., Cui J., Zhang H., Chen X., Li R., Wu N., Chen X., Wen S., Zhang J., Yin L., Deng F., Liao Z., Zhang Z., Zhang Q., Yan Z., Liu W., Ye J., Deng Y., Wang Z., Qiao M., Luu H.H., Haydon R.C., Shi L.L., Liang H, & He T.C. (2014). The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs). PLoS ONE, 9(5), e97316.
+ Open protocol
+ Expand
4

Immunofluorescence Staining of β-Catenin

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence staining was performed as described [30] (link), [40] (link), [43] (link), [50] , [53] (link), [54] (link). Briefly, cells were infected with AdWnt3A or AdGFP for 48 h, fixed with methanol, permeabilized with 1% NP-40, and blocked with 10% BSA, followed by incubating with β-catenin antibody (Santa Cruz Biotechnology). After being washed, cells were incubated with Texas Red-labeled secondary antibody (Santa Cruz Biotechnology). Stains were examined under a fluorescence microscope. Stains without primary antibodies, or with control IgG, were used as negative controls.
Deng F., Chen X., Liao Z., Yan Z., Wang Z., Deng Y., Zhang Q., Zhang Z., Ye J., Qiao M., Li R., Denduluri S., Wang J., Wei Q., Li M., Geng N., Zhao L., Zhou G., Zhang P., Luu H.H., Haydon R.C., Reid R.R., Yang T, & He T.C. (2014). A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly. PLoS ONE, 9(11), e113064.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!