Carbon coated copper grid

Manufactured by Electron Microscopy Sciences

Sourced in United States

Carbon-coated copper grids are a type of specimen support used in electron microscopy. They consist of a thin layer of carbon deposited on a copper mesh or grid. The carbon coating provides a stable and conductive surface for mounting and observing samples in an electron microscope.

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Lab products found in correlation

Orca side mounted camera, by Ametek (3 mentions) 831 bottom mounted ccd camera, by Ametek (3 mentions) Jem 1400 transmission electron microscope, by JEOL (2 mentions) Hydrochloric acid (hcl), by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Ammonium molybdate, by Electron Microscopy Sciences (2 mentions) Poly allylamine hydrochloride salt pah, by Merck Group (2 mentions) Glutaraldehyde, by Merck Group (2 mentions) Uranyl acetate, by Polysciences (2 mentions) Jem 3010, by JEOL (2 mentions) Lead citrate agents, by Merck Group (2 mentions) Spectramax m5, by Molecular Devices (2 mentions) Jem 1011 transmission electron microscope, by JEOL (2 mentions) Osmium tetroxide, by Merck Group (2 mentions) Tecnai 12 biotwin tem, by Thermo Fisher Scientific (1 mentions) Cytation 3, by Agilent Technologies (1 mentions) Eagle bottom mount camera, by Thermo Fisher Scientific (1 mentions) Tecnai 12 twin transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Lc325 cu, by Electron Microscopy Sciences (1 mentions) Edax apollo, by Ametek (1 mentions)

Close spelling variants of this product

Formvar/carbon-coated copper grids (51 citations) Copper grids (32 citations) Glow-discharged carbon-coated copper grids (22 citations) 300 mesh carbon-coated copper grids (17 citations) 400 mesh carbon-coated copper grids (16 citations) Carbon-coated 400 mesh copper grids (12 citations) Carbon-coated grids (11 citations) 200-mesh carbon-coated copper grid (10 citations) Glow-discharged formvar/carbon-coated copper grid (9 citations) Glow-discharged carbon-coated 400 mesh copper grids (9 citations)

45 protocols using carbon coated copper grid

Negative Staining of Hydrogel Samples

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Hydrogels were
first diluted 10-fold using ddH₂O and then negatively stained.
A carbon-coated copper grid (400 mesh from Electron Microscopy Sciences)
was placed on a 10 μL droplet of sample for 1 min and excess
liquid drained off using lint-free tissue. The grid was then placed
on a 10 μL droplet of ddH₂O for 10 s before excess
liquid was drained off. The grid was then transferred to a 10 μL
droplet of a 1% uranyl acetate solution for 30 s, and again excess
liquid was drained off. Finally, the grid was transferred to a 10
μL droplet of ddH₂O for 10 s before excess liquid
was drained off for a final time. The sample was then left to dry
before imaging using a FEI Tecnai12 BioTwin TEM at 100 keV.

Elsawy M.A., Smith A.M., Hodson N., Squires A., Miller A.F, & Saiani A. (2016). Modification of β-Sheet Forming Peptide Hydrophobic Face: Effect on Self-Assembly and Gelation. Langmuir, 32(19), 4917-4923.

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Comprehensive Characterization of PDL-FITC AuNPs

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PDL-FITC AuNPs were characterized using a combination of spectrophotometry (Cytation 3, Biotek), dynamic light scattering (DLS) and zeta potential (Malvern Zetasizer Nano-ZS), and transmission electron microscopy (TEM; FEI Tecnai). All PDL-FITC AuNP suspensions were sonicated for 20–30 seconds prior to analysis. Absorbance spectra were acquired to confirm PDL-FITC conjugation to AuNPs. DLS and zeta potential measurements, performed in DI water, were used to obtain size and charge information using standard methods. TEM samples were prepared by drop-casting PDL-FITC AuNPs on a plasma irradiated, carbon-coated copper grid (Electron Microscopy Sciences). The suspension was blotted with filter paper and the grid was allowed to fully air dry prior to imaging.

Hernandez D.S., Schunk H.C., Shankar K.M., Rosales A.M, & Suggs L.J. (2020). Poly-d-lysine coated nanoparticles to identify pro-inflammatory macrophages. Nanoscale Advances, 2(9), 3849-3857.

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Transmission Electron Microscopy of Nanoparticles

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TEM and Cryo-TEM images were captured on an FEI Tecnai 12 TWIN Transmission Electron Microscope, operating at 100 kV for TEM and 80 kV for cryo-TEM. 25 μM TEM samples were pipetted onto a carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, PA). Filter paper was used to wick away excess solution. 10uL of 2 wt% aqueous uranyl acetate was used to stain samples. For cryo-TEM, 25 μM samples were pipetted onto lacey carbon coated TEM grids (LC325-Cu, Electron Microscopy Sciences) pre-treated with plasma air to make the lacey carbon film hydrophilic. Samples were vitrified by plunging them into a liquid ethane reservoir precooled with liquid nitrogen. Both TEM and Cryo-TEM images were acquired with a 16 bit 2k × 2k FEI Eagle bottom mount camera (Hillsboro, OR). Images were processed with ImageJ (NIH, Bethesda, MD). Nanoparticle size was averages from three areas of view with more than 50 particles per image for TEM and 20 particles for Cryo-TEM. Bilayer thickness was averaged across three points along the circumference of the vesicle. Numbers are presented as averages ± 95% confidence interval.

Pastuszka M.K., Wang X., Lock L.L., Janib S.M., Cui H., DeLeve L.D, & MacKay J.A. (2014). An amphipathic alpha-helical peptide from Apolipoprotein A1 stabilizes protein polymer vesicles. Journal of controlled release : official journal of the Controlled Release Society, 191, 15-23.

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EDAX APOLLO Element Analysis Protocol

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The element analysis was conducted by EDAX APOLLO (EDAX Inc., Mahwah, NJ, USA). A 2 μL core-shell solution was added to a carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, PA, USA) and allowed to dry at room temperature.

Lu Y., Zhou T., You R., Wu Y., Shen H., Feng S, & Su J. (2018). Fabrication and Characterization of a Highly-Sensitive Surface-Enhanced Raman Scattering Nanosensor for Detecting Glucose in Urine. Nanomaterials, 8(8), 629.

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TEM Imaging of Nanomaterials

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Samples were prepared by depositing 7 μL of the appropriate solution onto a carbon-coated copper grid (Electron Microscopy Services, Hatfield, PA, USA), wicking away the excess solution with a small piece of filter paper. Next, 7 μL of a 2 wt % aqueous uranyl acetate solution was deposited and the excess solution was carefully removed as above to leave a very thin layer. The sample grid was then allowed to dry at room temperature prior to imaging. Bright-field TEM imaging was performed on a FEI Tecnai 12 TWIN Transmission Electron Microscope operated at an acceleration voltage of 100 kV. All TEM images were recorded by a SIS Megaview III wide-angle CCD camera.

Cheetham A.G., Zhang P., Lin Y.A., Lin R, & Cui H. (2014). Synthesis and Self-Assembly of a Mikto-Arm Star Dual Drug Amphiphile Containing both Paclitaxel and Camptothecin. Journal of materials chemistry. B, Materials for biology and medicine, 2(42), 7316-7326.

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Characterization of Peptide-Functionalized Nanoparticles

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The size and surface zeta potential of the nanoparticles were determined by dynamic light scattering (DLS, Malvern ZEN 3600 Zetasizer). For examining the morphology with transmission electron microscopy (TEM), the nanoparticles solution (10 μg mL⁻¹) was dropped on a carbon‐coated copper grid (400‐mesh, Electron Microscopy Sciences), washed with DI water, and stained with uranyl acetate (1 wt%, Sigma‐Aldrich). The grid was imaged with an FEI 200KV Sphera microscope. The peptide content on the ctLP‐NPs was quantified using a bicinchoninic acid (BCA) kit (Thermo Fisher Scientific) in reference to a bovine serum albumin (BSA) standard. The stability of bare PLGA cores, LP‐NPs, and ctLP‐NPs were measured by monitoring their sizes in 1× PBS over a period of 1 week.

Ai X., Duan Y., Zhang Q., Sun D., Fang R.H., Liu‐Bryan R., Gao W, & Zhang L. (2020). Cartilage‐targeting ultrasmall lipid‐polymer hybrid nanoparticles for the prevention of cartilage degradation. Bioengineering & Translational Medicine, 6(1), e10187.

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Transmission Electron Microscopy Imaging of Purified Vesicles

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10 µL of purified vesicles was placed onto a carbon‐coated copper grid (Electron Microscopy Services, Hatfield, PA, USA) for 10 min before being wicked away with a piece of filter paper. The grid was dipped in PBS twice to remove excess proteins from the media and was allowed to dry for 2 min. Next, uranyl acetate (10 µL of a 2 wt% solution) was placed on the grid for 1 min, before again being wicked away with filter paper. The grid was allowed to fully dry for 3 h to overnight at room temperature. Bright‐field TEM imaging was performed on a JEOL 1230 TEM. TEM operated at an acceleration voltage of 100 kV. All TEM images were recorded by a Hamamatsu ORCA side‐mounted camera or a Gatan 831 bottom‐mounted CCD camera, and AMT imaging software.

Gunnels T.F., Stranford D.M., Mitrut R.E., Kamat N.P, & Leonard J.N. (2022). Elucidating Design Principles for Engineering Cell‐Derived Vesicles to Inhibit SARS‐CoV‐2 Infection. Small (Weinheim an Der Bergstrasse, Germany), 18(19), 2200125.

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Staining and Imaging Samples for TEM

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A 5 μl aliquot of the sample to be analysed was placed on a carbon-coated copper grid (Electron Microscopy Sciences), incubated for 2 min and washed thrice with 7 μl H₂O. The grid was stained thrice with 5 μl 2% (w/v) uranyl acetate solution, dried and analysed using a JEM-1400 transmission electron microscope (Jeol), equipped with a 2 k × 2 k TVIPS TemCam-F216 camera.

Claus S., Puscalau-Girtu I., Walther P., Syrovets T., Simmet T., Haupt C, & Fändrich M. (2017). Cell-to-cell transfer of SAA1 protein in a cell culture model of systemic AA amyloidosis. Scientific Reports, 7, 45683.

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Negative Stain Electron Microscopy

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3 µl of freshly prepared protein (10 µg/ml) was applied to a glow-discharged (15 mA, 1 min, easiGlow; PELCO), homemade, carbon-coated copper grid (400 mesh; Electron Microscopy Sciences) and incubated for 60 s. The grid was washed four times with double-distilled H₂O, and excess water was removed by blotting after each step. The grid was dipped into 1.5% uranyl acetate stain for 1 s, then blotted and stained again for an additional 20 s. Excessive stain was removed by blotting, and the grid was air dried for 5 min. This protocol was adapted from Ohi et al. (2004) (link).
The prepared grids were imaged in an FEI Tecnai T12 electron microscope operated at 120 kV acceleration voltage, equipped with a Tietz F416 camera (2,048 × 2,048 pixels). Micrographs were obtained at a magnification of 49,000 with Leginon (Suloway et al., 2005 (link)), resulting in a pixel size of 3.44 Å/pixel. Particles were selected with DoG Picker (Voss et al., 2009 (link)), included in the Appion (Lander et al., 2009 (link); Voss et al., 2010 (link)) processing package. Extracted particle images were subjected to iterative rounds of 2-D classification with RELION 2.1 (Scheres, 2012 (link); Kimanius et al., 2016 (link)).

Schmidpeter P.A., Gao X., Uphadyay V., Rheinberger J, & Nimigean C.M. (2018). Ligand binding and activation properties of the purified bacterial cyclic nucleotide–gated channel SthK. The Journal of General Physiology, 150(6), 821-834.

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Transmission Electron Microscopy of NPs

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A drop of suspension containing NPs was deposited on a carbon-coated copper grid (Electron Microscopy Sciences, Hatfield, PA, USA) and allowed to dry at room temperature. Transmission electron microscopy was performed by using the low-/high-resolution transmission electron microscopy (TEM; JEM-2100, Tokyo, Japan; FEI F20, Columbus, OH, USA) at an accelerating voltage of 200 kV.

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