Dld 1

The WiDr, SW480, DLD-1, SW837, PANC-1, MIA PaCa-2, ASF 4-1, TIG-3-20, and KMST-6 cell lines were obtained from the Japanese Cancer Research Resources Bank. The SW48 cell line was from the Cell Resource Center for Biomedical Research of Tohoku University. The HT-29 and H9c2 cell lines were procured from the American Type Culture Collection. The BxPC-3 cell line was obtained from the European Collection of Authenticated Cell Cultures. RPMI-1640 medium (Wako Pure Chemicals Industries) used to culture SW48, WiDr, HT-29, SW480, DLD-1, SW837, and BxPC-3 cells; Eagle’s minimum essential medium (Wako) for MIA PaCa-2, PANC-1, ASF 4-1, and TIG-3-20 cells and Dulbecco’s minimum essential medium (Wako) for H9c2 cells. All media were supplemented with 10% (v/v) heat-inactivated FBS (Nichirei Biosciences, Tokyo, Japan), and cells were incubated under an atmosphere of 95% air and 5% CO₂ at 37°C. Testing for Mycoplasma contamination was performed using the activity of certain mycoplasmal enzymes (MycoAlert Mycoplasma detection kit; Lonza, Basel, Switzerland). The number of viable cells was assessed using the trypan blue dye exclusion test.

Human colorectal cancer cell lines DLD-1 and WiDr, human rhabdmyosarcoma cell line Rh30, human urinary bladder carcinoma cell lines T24 and 253J-BV, human stomach cancer cell lines KATOIII, NUGC3, and MKN7, and human prostate cancer cell line PC3 were maintained in RPMI-1640 medium (Wako Pure Chemical Industries, Ltd., Osaka, Japan), supplemented with 8% (v/v) heat-inactivated FBS (Sigma-Aldrich Co., St. Louis, MO, USA) and 2 mM l-glutamine. Human diploid fibroblast cell line ASF-4-1 and human rhabdmyosarcoma cell line RD were maintained in E-MEM medium (Wako), supplemented with 10% (v/v) heat-inactivated FBS and 2 mM l-glutamine. All cell lines were cultured under an atmosphere of 95% air and 5% CO₂ at 37 °C. DLD-1, WiDr, T24, 253J-BV, KATOIII, MKN7, RD, and ASF-4-1 cell lines were obtained from the JCRB cell bank (Osaka, Japan). The PC3 cell line was purchased from the American Type Cell Collection (ATCC). The Rh30 and NUGC3 cell lines were provided by Dr. Hosoi (Kyoto Prefectural University of Medicine) and Taiho Pharmaceutical (Tokyo, Japan), respectively. The number of viable cells was determined by performing the trypan-blue (Life Technologies, Carlsbad, CA, USA) dye exclusion test.