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Mtt kit

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The MTT kit is a colorimetric assay used to measure cell viability and proliferation. It utilizes the yellow tetrazolium dye MTT, which is reduced to purple formazan crystals by metabolically active cells. The formazan crystals are then solubilized, and the absorbance is measured, providing a quantitative assessment of cell number and metabolic activity.

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Lab products found in correlation

Microplate reader, by Thermo Fisher Scientific (6 mentions) Microplate reader, by Bio-Rad (2 mentions) Microplate absorbance reader, by Thermo Fisher Scientific (2 mentions) Fluorescence imaging system, by Bio-Rad (1 mentions) Microscopic imaging system, by Leica (1 mentions) Puerarin, by Merck Group (1 mentions) Rna extraction kit, by Takara Bio (1 mentions) Phosphorylated erk1 2 p erk1 2, by Abcam (1 mentions) Mmp 9, by Abcam (1 mentions) Matrigel, by BD (1 mentions) Erk1 2, by Abcam (1 mentions) 96 well plate, by Corning (1 mentions) Multimode plate reader, by Bio-Rad (1 mentions) Prism 5, by GraphPad (1 mentions) Docetaxel, by Merck Group (1 mentions) Thymoquinone, by Merck Group (1 mentions) Dspe peg2000, by Avanti Polar Lipids (1 mentions) Cholesterol, by Avanti Polar Lipids (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Fas antibody, by BD (1 mentions)

Spelling variants of this product

MTT assay kit (39 citations) CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) kit (19 citations) Celltiter 96 non-radioactive MTT assay kit (4 citations) MTT cell proliferation assay kit (4 citations) MTT colorimetric assay kit (3 citations) Non-radioactive MTT assay kit (2 citations) MTT proliferation assay kit (2 citations) CellTiter 96 MTT assay kit (2 citations) Modified MTT assay kit (2 citations)

19 protocols using mtt kit

1

Evaluation of GRA's Anti-Proliferative Effects

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The anti-proliferative effects of GRA were determined using MTT assay as previously reported [46 (link)]. The gastric tumor cell lines BGC-823 and MKN-1 were seeded in 96-well plates and treated with serial dilutions of GRA (0, 50, 100, 150, 200 μM) (n = 10) for 4 h, 24 h and 48 h. The immortal gastric epithelia cell line GES-1 was used as a control. Cell viability was assessed using an MTT Kit (Promega, Madison, USA) and absorbance was measured at 490 nm using a micro plate reader (Thermo, MA, USA).
Cao D., Jia Z., You L., Wu Y., Hou Z., Suo Y., Zhang H., Wen S., Tsukamoto T., Oshima M., Jiang J, & Cao X. (2016). 18β-glycyrrhetinic acid suppresses gastric cancer by activation of miR-149-3p-Wnt-1 signaling. Oncotarget, 7(44), 71960-71973.
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2

MTT-based Cell Proliferation Assay

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Cell proliferation was evaluated with an MTT kit (Promega) complying with manufacturer's instructions. Thereafter, 3 × 103 cells were seeded in each well of a 24‐well plate and maintained in medium containing 10% FBS for 2 weeks, during which time the medium was replaced with MTT reagent every 4 days. Cell proliferation and viability were measured with absorbance at 490 nm.
Chen W., Li Q., Zhang G., Wang H., Zhu Z, & Chen L. (2020). LncRNA HOXA‐AS3 promotes the malignancy of glioblastoma through regulating miR‐455‐5p/USP3 axis. Journal of Cellular and Molecular Medicine, 24(20), 11755-11767.
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3

Evaluating Neuronal Cell Viability with Aβ1-42

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The viability of SH-SY5Y and SK-N-SH cells with or without Aβ1-42 treatment was detected by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit (Promega Corporation, Madison, WI, U.S.A.). Briefly, SH-SY5Y and SK-N-SH cells (5Cor4 cells/well) were treated with different concentrations of Aβ1-42 (0, 5, 10, 20 μM) for 24 h or Aβ1-42 (10 μM) for different times (0, 12, 24, 48 h). Subsequently, MTT (20 μl, 5 mg/ml) was added to each well and maintained for 4 h. Next, dimethyl sulfoxide solution was added to dissolve the formazan crystals. The optical density (OD) at 490 nm was measured using the Microplate Reader (Thermo Fisher Scientific).
Xu W., Li K., Fan Q., Zong B, & Han L. (2020). Knockdown of long non-coding RNA SOX21-AS1 attenuates amyloid-β-induced neuronal damage by sponging miR-107. Bioscience Reports, 40(3), BSR20194295.
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4

MTT Assay for Cell Viability

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A 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit was purchased from Promega (USA). MMP9, ERK1/2, and phosphorylated ERK1/2 (p ERK1/2) antibodies were purchased from Abcam (USA). An RNA extraction kit, reverse transcription kit, and quantitative polymerase chain reaction (qPCR) SuperMIX were purchased from TAKARA (Japan). Puerarin was purchased from Sigma (USA). Matrigel was purchased from BD (USA). The gene amplification instrument and fluorescence imaging system were obtained from Bio-RAD (USA). A microscopic imaging system (Leica, Germany) from Germany was also used.
Lang J., Guo Z., Xing S., Sun J., Qiu B., Shu Y., Wang Z, & Liu G. (2022). Inhibitory role of puerarin on the A549 lung cancer cell line. Translational Cancer Research, 11(11), 4117-4125.
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5

MTT Cell Proliferation Assay

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A cell proliferation assay was carried out with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit (Promega Corporation) following manufacturer’s recommendations. MDA-MB-231 cells were allowed to grow in a 96-well plate with 2×104 cells/well and then transfected with different plasmids for 24 h. Next, 50 µL MTT (5 mg/mL) was added and incubated for 4 h in a humidified atmosphere containing 5% CO2 at 37°C. The reaction was then terminated by removal of the supernatant without disturbing the cells and 150 µL of dimethylsulfoxide (DMSO) solution was added to the wells. After continuous shaking at room temperature for 15 min to dissolve the purple formazan crystals, the optical density (OD) at a wavelength of 490 nm of each well was examined with a microplate reader (Bio-Rad Laboratories Inc.).
Dong Y., Chang C., Liu J, & Qiang J. (2017). Targeting of GIT1 by miR-149* in breast cancer suppresses cell proliferation and metastasis in vitro and tumor growth in vivo. OncoTargets and therapy, 10, 5873-5882.
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6

Lipotoxicity effects on MIN6 cells

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The viability of MIN6 cells expressing or not expressing CAV1 in lipotoxic in vitro conditions was assessed using the MTT kit (Promega, Madison, WI, USA). In brief, MIN6 cells were seeded in 96-well plates for 24 h, and then different concentrations (0.25, 0.5 and 1.0 mM) of palmitate or oleate were added for 24 h. At the end of this period, the MTT reagent was applied, following the manufacturer’s instructions. In the same way, viability was assessed in cells pre-incubated with MAPK inhibitors.
Lillo Urzúa P., Núñez Murillo O., Castro-Sepúlveda M., Torres-Quintana M.A., Lladser Caldera Á., Quest A.F., Espinoza Robles C., Llanos Vidal P, & Wehinger S. (2020). Loss of Caveolin-1 Is Associated with a Decrease in Beta Cell Death in Mice on a High Fat Diet. International Journal of Molecular Sciences, 21(15), 5225.
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7

MTT Proliferation Assay for Neuroblastoma

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The MTT kit from Promega (Madison, WI, USA) was applied to assess proliferation NB cells. In short, the transfected NB cells (3×103 cells/well) were inoculated into 96-well plates (Corning Costar, Corning, NY, USA) and cultured at 37°C constant temperature with 5% CO2 for 24 h, 48 h and 72 h. Then, each well inoculated with transfected NB cells was added with 20 μL of MTT solution (5 mg/mL) at the appointed time and maintained for 4 h. The supernatants were discarded and dimethyl sulfoxide solution (100 μL) was replenished for the dissolution of the formazan crystal. In the end, the Microplate Absorbance Reader from Thermo Fisher Scientific (Waltham, MA, USA) was conducted to determine the color reaction at 490 nm.
Wen Y., Gong X., Dong Y, & Tang C. (2020). Long Non Coding RNA SNHG16 Facilitates Proliferation, Migration, Invasion and Autophagy of Neuroblastoma Cells via Sponging miR-542-3p and Upregulating ATG5 Expression. OncoTargets and therapy, 13, 263-275.
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8

Cell Proliferation Assay using MTT

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Cell proliferation was determined 48 h after transfection treatment using a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit (Promega Corporation) in a 96-well plate. The results were subsequently analyzed at 0, 1, 2 and 3 day. For the analysis, first, the cell culture medium was aspirated from the 96-well plate, and the cells were gently washed with PBS twice, followed by the addition of 20 µl of 5 mg/ml MTT reagent. After 4 h of incubation, MTT was carefully removed without disturbing the cells and 150 µl of dimethylsulfoxide (DMSO) solution was added to the wells, which was followed by continuous shaking at room temperature for 15 min to solubilise the purple formazan crystals. Finally, the optical density of each well, including the blank well without cells, was measured at a wavelength of 490 nm in a multimode plate reader (Bio-Rad, USA). Each sample was assayed in triplicate.
Liu J., Chen Z., Xiang J, & Gu X. (2018). MicroRNA-155 acts as a tumor suppressor in colorectal cancer by targeting CTHRC1 in vitro. Oncology Letters, 15(4), 5561-5568.
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9

MTT Assay for Anticancer Potency

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MTT assay was performed using HeLa, PC-3, LNCaP, parental HCT116, and HCT116HIF–1α–/–HIF–2α–/– cells. The HCT116 parental and knockout cells and the PC-3 and LNCap cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) or Roswell Park Memorial Institute Medium (rpmI1640) containing 10% fetal bovine serum and 100 U/mL penicillin and streptomycin and maintained at 37°C in a humidified incubator under 5% CO2. The cells (PC-3, 5×l03; LNCaP, HCT116 parental and knockout cells 8×103; HeLa, 104 cells/well) were seeded onto a 96-well plate and incubated overnight. Varying concentrations of purified adametizine A (1; up to 100 μM) were added to the wells. After incubation at 37°C for 48 or 72 h (HeLa), the cell viability was determined by MTT kit (Promega), following manufacture’s protocols. Alternatively, 10 μL of MTT (five mg/mL) in PBS was added and incubated for 4 h, followed by the aspiration of the medium. Dimethyl sulfoxide (DMSO, 100 μL) was added to each well to dissolve the MTT in the wells and the plate was agitated for 1 h. The optical density (OD) was measured at 570 nm using a UV/vis microplate spectrophotometer (BioTek or Spectramax). Three to six replications were performed per treatment. The IC50 value was determined by analyzing data with Excel or GraphPad Prism 5 (GraphPad Software, Inc.).
Jiang G., Zhang P., Ratnayake R., Yang G., Zhang Y., Zuo R., Powell M., Huguet-Tapia J.C., Abboud K.A., Dang L.H., Teplitski M., Paul V., Xiao R., Ahammad K.H., Zaman U., Hu Z., Cao S., Luesch H, & Ding Y. (2020). Fungal Epithiodiketopiperazines Carrying α,β-Polysulfide Bridges from Penicillium steckii YE, and Their Chemical Interconversion. Chembiochem : a European journal of chemical biology, 22(2), 416-422.
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10

MTT Cell Viability Assay

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Cells were cultured in 96-well plates with DMEM containing 10% FBS. After HEC-23 treatment for 24 h, 15 μL of dye solution was added into each well. Then the plates were incubated at 37°C for 2 h in a humidified CO2 incubator. 100 μL of stop solution was added to each well, and the absorbance was recorded at 570 nm using a 96-well plate reader. A reference wavelength at 630 nm was used. The MTT kit was purchased from Promega (Cat# G4002).
Li Y., Zhang Y., Gan Q., Xu M., Ding X., Tang G., Liang J., Liu K., Liu X., Wang X., Guo L., Gao Z., Hao X, & Yang C. (2018). C. elegans-based screen identifies lysosome-damaging alkaloids that induce STAT3-dependent lysosomal cell death. Protein & Cell, 9(12), 1013-1026.
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