Inveon multimodality system

During pouring of the TMM into the fixture, TC wires could be displaced slightly from their original position. As a result, high resolution micro-CT (Inveon Multimodality System, Siemens Inc., Germany) was used to scan the tissue phantoms, in order precisely determine the location of the TC junctions, which are later used in the inverse algorithm [17 (link)]. The processing software used in conjunction with the CT scan images was Inveon Research Workplace 4.2. The acquisition software used for obtaining the CT images was Inveon Acquisition Workplace (IAW) 2.0.2. The micro-CT images obtained with a voxel size of 36 μm were brighter for tissue phantoms with higher mNPs concentration compared to the control phantom. Fig 3 shows the TC junctions as bright spots inside the phantom. The position of these junctions were determined for the control phantom and the phantoms containing 0.0047% and 0.047% mNPs. The TC junction locations were later used in determining the focal location of the beam.

In this study, Micro-CT (Inveon Multimodality System, Siemens Medical Solutions, Malvern, PA, USA) was used for microstructural analysis of cancellous bone samples collected from the rib area of pigs, which were obtained from local commercial slaughterhouses. Figure 1 shows the extraction process of related parameters of bone trabeculae. The selected bone trabeculae samples were divided into three layers for extraction and then divide each layer into five 2 mm sided cubes. A separate color was assigned to each segmented model for later statistical analysis. A total of 10 bone trabecular models were selected and 150 samples were extracted for reconstruction and parameter measurement. The porosity of each model was counted in the range of 40% to 80% in 5% increments. The results were shown in Figure 1. The results showed that the average porosity of the block mode l was 58.9%, which was concentrated in the range of 55–65%, accounting for 83.4% of the total samples. Therefore, in this study, the porosity of the bionic scaffold was selected as 55–65%, and the porosity of the test model was determined to be 55%, 57%, 59%, 61%, 63%, 65% at the interval of 2%. The average thickness of bone trabeculae in the region was calculated to be 0.209 ± 0.004 mm.

The NOTA was purchased from Macrocyclic, Inc; protected amino acids, other peptide synthesis reagents, and resins were obtained from Nanchang tanzhenbio Co., Ltd; anhydrous aluminum chloride (AlCl₃) and sodium acetate were purchased from Alfa Aesar (China) Chemicals Co., Ltd; and trifluoroacetic acid (TFA) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (China). Reagents, including phosphate-buffered saline (PBS) and cell culture medium, were obtained from Sigma-Aldrich. Fetal bovine serum (FBS) was purchased from Biological Industries (BI) company. All reagents and solvents were commercial products and used without further purification. The Siemens Cyclotron produced [¹⁸F] fluoride by bombarding a [¹⁸O] H₂O target with 12.5 MeV protons. Analytical HPLC (high-performance liquid chromatography) was performed on an Agilent 1200 system with a reversed-phase column (Agilent Zorbax ODS, 250 × 4.6 mm). Mass spectra were obtained on a Q-T of a premier UPLC system equipped with an electrospray interface (ESI) (Waters, USA). A γ-counter (CAPRA-R, Capintec, Inc., Ramsey, New Jersey, USA) was used to measure the radiation value of the biological distribution experiment. Small animal imaging was performed using a micro-PET/CT scanner (Siemens Inveon Multimodality System, Germany).