N hydroxysulfosuccinimide sodium salt

Manufactured by Merck Group

Sourced in United States, France

N-hydroxysulfosuccinimide sodium salt is a chemical compound used as a reagent in various laboratory applications. It functions as a water-soluble, anionic N-hydroxysuccinimide (NHS) derivative. This compound is commonly utilized in coupling reactions and bioconjugation procedures.

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N-hydroxysulfosuccinimide (38 citations) N-hydroxysulfosuccinimide sodium salt (sulfo-NHS) (26 citations) N-hydroxysulfosuccinimide sodium salt (NHS) (10 citations) N- hydroxysulfosuccinimide sodium (NHS) (3 citations) N-hydroxysulfosuccinimide sodium salt (S-NHS) (2 citations)

36 protocols using n hydroxysulfosuccinimide sodium salt

Synthesis and Functionalization of UCNPs

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Yttrium acetate tetra-hydrate (99.9%), ytterbium acetate hydrate (99.99%), thulium acetate hydrate (99.9%), neodymium acetate hydrate (99.9%), oleic acid (technical grade, 90%), 1-octadecene (technical grade, 90%), ammonium fluoride (≥98%), sodium hydroxide (reagent grade, ≥98%), ethanol, cyclohexane, branched poly(ethylenimine) (mol. weight 25 000), N,N-dimethylformamide (anhydrous, ≥99.8%), nitrosonium tetrafluoroborate (>98%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, >99%), N-hydroxysulfosuccinimide sodium salt (≥98%), and fluorescein-5-isothiocyanate (FITC) were purchased from Sigma-Aldrich and used as received, without any further purification. Sodium phosphate buffer solution of pH 7–8 (100 mM) and sodium citrate with citric acid buffer (100 mM) of pH 3–6 were purchased from LPS Solution (Daejeon, South Korea) for use in the pH-dependent experiments. The water used in the synthesis was purified with a Milli-Q filtration system before use. The radius of the rotator of the centrifuge machine was 19 cm and rcf was 3399 × g (Combi 514R, Hanil Science Medical) for the centrifugation of the UCNPs' synthesis. The centrifugation force (rcf = 11 620 × g) was applied for the purification of FITC-conjugated PEI-UCNPs in a centrifuge (SLCF-10, SeouLin Bioscience Co. Ltd).

Mahata M.K, & Lee K.T. (2019). Development of near-infrared sensitized core–shell–shell upconverting nanoparticles as pH-responsive probes. Nanoscale Advances, 1(6), 2372-2381.

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Nisin A-Based Antimicrobial Hydrogel Synthesis

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Nisin A (95%, isolated from Lactococcus lactis in sauerkraut) was obtained from Handary, Belgium. Dextran (from Leuconostoc mesenteroides, 100–200 kDa), Dextran 60 (from L. mesenteroides, ~ 60 kDa), alginic acid (from brown algae, low viscosity (4–12 cps), M/G ratio 1.43 as determined by ¹³C SS-NMR below), sodium (meta) periodate (> 99%), ethylene glycol (99%), N-hydroxysulfosuccinimide sodium salt (S-NHS, > 98%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, crystalline), adipic acid dihydrazide (ADH, > 98%), hydroxylamine hydrochloride, glycol chitosan (degree of deacetylation: > 60%), trifluroacetic acid (TFA, > 99%), acetonitrile (ACN, > 99.9%), phosphate-buffered saline (tablets, PBS), and sodium dodecyl sulphate were all purchased from Sigma-Aldrich, Ireland. Dimethyl sulfoxide (DMSO, for molecular biology) was purchased from VWR International. Liquid nitrogen was supplied by BOC Gases (Ireland). Glycine (lab grade), 2,4,6-trinitrobenzene sulfonic acid (5% w/v, MeOH), potassium chloride (99%) and hydrochloric acid (37%, fuming) were purchased from Fisher Scientific, Ireland. Pullulan/Dextran standards for light scattering/tetra detection (TDS3030) were provided by Particular Sciences, Dublin, Ireland. Staphylococcus aureus (DSM 20231) was purchased from the Leibniz Institute DSMZ-German collection of Microorganisms and Cell Cultures.

Flynn J., Culebras M., Collins M.N, & Hudson S.P. (2022). The impact of varying dextran oxidation levels on the inhibitory activity of a bacteriocin loaded injectable hydrogel. Drug Delivery and Translational Research, 13(1), 308-319.

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Characterization of Hydrophobic Intraocular Lens Materials

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MPC, 4-cyano-4-(phenyl-carbonothioylthio) pentanoic acid (CPCTTPA), 2-morpholino-ethanesulfonic acid, 4,4′-azobis-(4-cyanovaleric acid) (V501), (3-aminopropyl) triethoxysilane (APTES), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (NHSS), and fluorescein diacetate were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). LIVE/DEAD BacLight bacterial viability kit (L-7012) was supplied by Invitrogen. Masson’ Trichrome staining kits were purchased from Leagene Bio-tech Co., Ltd (Beijing, People’s Republic of China). Silicone (polydimethylsiloxane, PDMS), prepared from Sylgard^® 184 of Dow Corning (Midland, MI, USA), was used as hydrophobic IOL material substrate for characterization.21 Foldable IOL for animal experiments was supplied by Alcon (Fort Worth, TX, USA; AcrySof^® SN60WF; diameter of optic: 6 mm). Human LEC line (HLE B3, CRL-11421™) was originally obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA). The clinical postoperative administration drugs were obtained from Eye Hospital of Wenzhou Medical University.

Han Y., Xu X., Tang J., Shen C., Lin Q, & Chen H. (2016). Bottom-up fabrication of zwitterionic polymer brushes on intraocular lens for improved biocompatibility. International Journal of Nanomedicine, 12, 127-135.

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Fabrication of Hyaluronic Acid-Based Hydrogel

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Metronidazole (Pharmaceutical Secondary Standard), vancomycin (Van, >90%), 1-(3-dimethylaminopropyl)−3-ethylcarbodiimide hydrochloride (EDC, >99%), N-hydroxysulfosuccinimide sodium salt (NHS, >98%), hyaluronidase (Hyal, 400 units/mg), fibrinogen, thrombin (~120 units/mg), dimethyl sulfoxide (DMSO, >99.9%), dimethylformamide (DMF, >99.9%), 9, 10-anthracenediyl-bis(methylene)-dimalonic acid (ABDA, >90%), and ethylenediamine (>99.5%) were obtained from Sigma-Aldrich; sodium hyaluronate (200 kDa) was purchased from SunlidaBio; and Ce6 (>99%) was bought from JenKem Technology.

Xiu W., Wan L., Yang K., Li X., Yuwen L., Dong H., Mou Y., Yang D, & Wang L. (2022). Potentiating hypoxic microenvironment for antibiotic activation by photodynamic therapy to combat bacterial biofilm infections. Nature Communications, 13, 3875.

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Carboxylated Magnetic Bead Acquisition and Characterization

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Acquisition of Carboxylated magnetic beads (10 mg/mL, 180 nm) was sourced from Shanghai Allrun Nano Science & Technology Co. Ltd. Sigma Aldrich Chemical Co (St. Louis, U.S.A.). served as the supplier of N-Hydroxysulfosuccinimide sodium salt (NHSS) and 1-(3-(dimethylamino) propyl)-3-ethylcarbodiimide hydrochloride (EDC). Penicillin was bought from Shanghai Aladdin Industrial Corporation. Besides, LB medium was obtained from Beijing Land Bridge Technology Co. Ltd. Columbia blood agar plates were supplied by Zhengzhou Autobio Biotechnology Co. Ltd. A solution of 0.01 M PBS (0.137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.4), was provided by Beijing Solarbio Science & Technology Co. Ltd. The Department of Clinical Laboratory, Jiangxi Maternal and Child Health Hospital, Nanchang, China was the provider of urine samples.

Tang X., Fan L.P, & Liu Y. (2023). Quantitative real-time PCR and magnetic separation strategy for specific detection of group B streptococcus in perinatal Women's urine. Practical Laboratory Medicine, 38, e00348.

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Carboxyl-Functionalized Polystyrene Particle Conjugation

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N-Ethyl-N-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), sodium dodecyl sulfate (SDS), N-Hydroxysulfosuccinimide sodium salt (NHS) and sNHS-biotin were obtained from Sigma Aldrich. NHS-PEG

_{12}

was obtained from Iris Biotech. Carboxyl functionalized polystyrene particles (20 nm) 8.86 × 10

^{14}

particles/mL were obtained from Nanocs Inc. (New York, NY, USA) Buffers: MES-T Buffer pH 6: 10 mM MES (2-(N-morpholino)ethanesulfonic acid)), 0.05% Tween20. PB-T pH 7: 100 mM H

_{2}

NaPO

_{4}

, 0.05% Tween20. HBS-T pH 7.5: 50 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), 150 mM NaCl, 0.1% Tween20. HBS-T pH 8: 10 mM HEPES, 150 mM NaCl, 0.05% Tween20.

Frutiger A., Gatterdam K., Blickenstorfer Y., Reichmuth A.M., Fattinger C, & Vörös J. (2020). Ultra Stable Molecular Sensors by Submicron Referencing and Why They Should Be Interrogated by Optical Diffraction—Part II. Experimental Demonstration. Sensors (Basel, Switzerland), 21(1), 9.

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Silica-Coated Magnetic Nanoparticles for miRNA-122 Detection

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N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC); N-Hydroxy-sulfosuccinimide sodium salt (NHS); 3-(N-Morpholino)propanesulfonic acid sodium salt (MOPS); tetraethoxyorthosilicate (TEOS); and 3-(aminopropyl)triethoxysilane (APTS) were purchased from Sigma-Aldrich (St Quentin Fallavier, France). Citric acid was purchased from Merck (Nogent sur Marne, France). 2-[Methoxy(polyethyleneoxy)propyl]-trimethoxysilane (PEOS), containing 3-6 ethylene oxide groups, was purchased from Gelest (Morrisville, PA, USA). 30% H₂O₂, HCl and NaOH solutions were obtained from VWR (Strasbourg, France). The oligonucleotides were purchased from Integrated DNA Technology (Leuven, Belgium). The probe was 21-basis long and was modified with a carboxy-end for conjugation to silica coated magnetic nanoparticles. The target was an unmodified DNA sequence of 20 bases as written in Table 1 mimicking the fragment of interest, miRNA-122, for the diagnosis of liver cells in case of injury (hepatitis, alcoholism, obesity).

Horny M.C., Gamby J., Dupuis V, & Siaugue J.M. (2021). Magnetic Hyperthermia on γ-Fe2O3@SiO2 Core-Shell Nanoparticles for mi-RNA 122 Detection. Nanomaterials, 11(1), 149.

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Functionalized Silica Nanoparticle Synthesis

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Sodium chloride (NormaPur), methylene blue (Alfa Aesar), potassium ferricyanide (III), and potassium hexacyanoferrate (II) (Sigma-Aldrich, St Quentin Fallavier, France) were used in the experiments without further purification. N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC); N-Hydroxy-sulfosuccinimide sodium salt (NHS); 3-(N-Morpholino)propanesulfonic acid sodium salt (MOPS) and tetraethoxyorthosilicate (TEOS); 3-(aminopropyl)triethoxysilane (APTS) were purchased from Sigma–Aldrich (St Quentin Fallavier, France). Citric acid was purchased from Merck (Nogent sur Marne, France). 2-[Methoxy(polyethyleneoxypropyl]-trimethoxysilane (PEOS), containing 3–6 ethylene oxide groups, was purchased from Gelest (Morrisville, PA, USA). From VWR (Strasbourg, France) 30% H₂O₂, HCl and NaOH solutions were obtained.

Horny M.C., Dupuis V., Siaugue J.M, & Gamby J. (2020). Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip. Sensors (Basel, Switzerland), 21(1), 185.

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Radiolabeling of Peptides with Gallium-68

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⁶⁸Ga (t½ = 68 min, β += 89% and EC = 11%) was obtained from a ⁶⁸Ge/⁶⁸Ga generator system (ITG Isotope Technologies Garching GmbH, Germany) in which ⁶⁸Ge (T½ = 270 d) was attached to a column based on organic matrix generator. The ⁶⁸Ga was eluted with 4 mL of 0.05 M hydrochloric acid. Iron (III) chloride, hydrazine monohydrate, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride and N-hydroxysulfosuccinimide sodium salt were purchased from Sigma-Aldrich. Citric acid trisodium salt dihydrate was purchased from Acros organics. cFLFLF peptide was purchased from Biomedal.

Pellico J., Lechuga-Vieco A.V., Almarza E., Hidalgo A., Mesa-Nuñez C., Fernández-Barahona I., Quintana J.A., Bueren J., Enríquez J.A., Ruiz-Cabello J, & Herranz F. (2017). In vivo imaging of lung inflammation with neutrophil-specific 68Ga nano-radiotracer. Scientific Reports, 7, 13242.

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Gold Nanoparticle Functionalization Protocol

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Gold chloride hydrate and CF300-Cu-50 transmission electron microscopy (TEM) grids were purchased from Electron Microscopy Services. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), O-(2-carboxyethyl)-O′-(2-mercaptoethyl)heptaethylene glycol (HEG), sodium citrate, calcein, sodium phosphate, Triton X-100, CL-4B sepharose, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-hydroxy sulfo-succinimide sodium salt (NHS) were purchased from Sigma-Aldrich. Polystyrene disposable cuvettes were purchased from VWR. Millipore deionized water was used in all experiments without further treatment. All reagents and solvents were used as is without further purification.

Zheng Z., Saar J., Zhi B., Qiu T.A., Gallagher M.J., Fairbrother D.H., Haynes C.L., Lienkamp K, & Rosenzweig Z. (2018). Structure–Property Relationships of Amine-rich and Membrane-Disruptive Poly(oxonorbornene)-Coated Gold Nanoparticles. Langmuir : the ACS journal of surfaces and colloids, 34(15), 4614-4625.

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