HPLC was performed using a JASCO LC-2000 Plus series instrument (JASCO, Tokyo, Japan) equipped with an intelligent UV-VIS detector (UV-2075 Plus), a quaternary gradient pump (PV-2089 Plus), an intelligent column oven (CO-2065 Plus), an intelligent autosampler (AS-2057 Plus), an LC-Net II/ADC user interface, and ChromNAV data analysis software. A COSMOSIL Cholester column (4.6 × 250 mm, particle size 5 μm, Nacalai Tesque INC. Kyoto, Japan) was used for HPLC analyses at 40°C. The mobile phase was a 40:60 (v/v) mixture of 0.1% formic acid (Wako Pure Chemical Industries, Ltd., Osaka, Japan) in acetonitrile (Nacalai Tesque Inc., Kyoto, Japan) and 0.1% formic acid in water. The flow rate was 1 mL/min, the injection volume was 10.0 μL, and the detection wavelength was 343 nm. The CM peak was observed at 11.1 min.
Co 2065 plus
Manufactured by Jasco
Sourced in Japan
The CO-2065 Plus is a laboratory instrument designed for the measurement of carbon dioxide (CO2) levels. It features a compact and durable design, and utilizes non-dispersive infrared (NDIR) technology to provide accurate and reliable CO2 readings.
Automatically generated - may contain errors
Lab products found in correlation
Uv 2075 plus, by Jasco (2 mentions)
Lsm 780, by Zeiss (2 mentions)
Acetonitrile, by Nacalai Tesque (1 mentions)
Formic acid, by Nacalai Tesque (1 mentions)
Formic acid, by Fujifilm (1 mentions)
Cosmosil cholester column, by Nacalai Tesque (1 mentions)
As 2057 plus, by Jasco (1 mentions)
400 spectrometer, by Bruker (1 mentions)
Autoflex speed instrument, by Bruker (1 mentions)
Avance 3 600 spectrometer, by Bruker (1 mentions)
Microtof q 2, by Bruker (1 mentions)
Pu 2089 plus, by Jasco (1 mentions)
Uv 2070 plus, by Jasco (1 mentions)
Extrema, by Jasco (1 mentions)
Jmtc 400 54 ss, by JEOL (1 mentions)
Mx 2080 32, by Jasco (1 mentions)
Md 2015 plus, by Jasco (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Pu 2080 plus, by Jasco (1 mentions)
6 protocols using co 2065 plus
1
HPLC Analysis of Cholesterol-Containing Compounds
2
Characterization of Novel Compounds
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The general chemicals were purchased from Fujifilm Wako Pure Chemical, the Tokyo Chemical Industry, Kanto Chemical or Aldrich. The target DNAs and RNAs were purchased from JBioS (Japan) (Table 1 ). The 1H NMR spectra (400 MHz) were recorded by a Bruker 400 spectrometer. The 1H NMR spectra (600 MHz) and 13C NMR spectra (150 MHz) were recorded by a Bruker AVANCE III 600 spectrometer. The high resolution electrospray mass analysis was performed by a Bruker MicrOTOFQ II. The HPLC purification was performed by a JASCO HPLC System (PU-2089Plus, UV-2075Plus, FP-2015Plus and CO-2065Plus). A reverse-phase C18 column (COSMOSIL 5C18-AR-II, Nacalai tesque, 4.6 × 250 mm or 10 × 250 mm for ligand purification). MALDI-TOF MS measurements were performed by a Bruker Autoflex speed instrument using a 3-hydroxypicolinic acid/diammonium hydrogen citrate matrix.
3
Cheddar Cheese Amino Acid Profile
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The free amino acid constitution of commercial Cheddar cheese and the best cheese variety which resulted using PF 5.85, Conc. of pure protease 0.01% (v/v) and 0.6 month ripened at 10 °C were assessed by using a system called gradient HPLC (Jasco CO-2065 Plus) with pre-column phenylisothiocyanate (PITC) derivatisation (Rozan et al. 2000 (link)). Concentration of every amino acid was denoted as mg/g of sample.
4
Comprehensive Polymer Characterization and Intravital Imaging
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Molecular weight and molecular weight distribution were measured by high-performance liquid chromatography (HPLC; EXTREMA, JASCO, Tokyo, Japan) with an intelligent sampler (AS-2051Plusm, JASCO, Tokyo, Japan), intelligent HPLC pump (PU-2080 Plus, JASCO, Tokyo, Japan), dynamic mixer (MX-2080-32, JASCO, Tokyo, Japan), 4-Line Degasser (DG-2080-54, JASCO, Tokyo, Japan), intelligent column oven (CO-2065 Plus, JASCO, Tokyo, Japan), multiwavelength detector (MD-2015 Plus, JASCO, Tokyo, Japan), intelligent UV/VIS detector (UV-2070 Plus, JASCO, Tokyo, Japan), and an LC-NetⅡ/ADC (JASCO, Tokyo, Japan). The chemical composition of the synthesized polymers was evaluated by 1H nuclear magnetic resonance spectroscopy (JMTC-400/54/SS, JEOL, Tokyo, Japan). The radius of the molecules was evaluated by dynamic light scattering (Zetasizer Nano-ZS, Malvern Instruments, Malvern, United Kingdom) using a laser with a wavelength of 532 nm. The images of the cells were obtained by confocal laser scanning microscopy (CLSM; LSM 780, Carl Zeiss, Germany). Intravital CLSM was performed using a Nikon A1R (Nikon, Japan).
5
Amino Acid Profile and Volatile Compounds in Cheddar Cheese
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The free amino acid constitution of commercial Cheddar cheese and the best cheese variety which resulted using PF 5.85, Conc. of pure protease 0.01% (v/v) and 0.6 month ripened at 10 °C were assessed by using a system called gradient HPLC (Jasco CO-2065 Plus) with pre-column phenylisothiocyanate (PITC) derivatisation (Rozan et al. 2000) . Concentration of every amino acid was denoted as mg/g of sample.
Volatile Flavor Compounds Release in Cheese Sample preparation (SPME Procedures)
The static headspace solid-phase micro-extraction method (HS-SPME) were used to remove the volatile elements (Verzera et al. 2010) . The ideal cheese using PF 3.12, Conc. of pure protease 0.01% (v/v) and 0.6/3 months ripened at 10 °C and commercial Cheddar cheese were analysed for fragrance volatiles by the SPME-GC-MS technique.
Volatile Flavor Compounds Release in Cheese Sample preparation (SPME Procedures)
The static headspace solid-phase micro-extraction method (HS-SPME) were used to remove the volatile elements (Verzera et al. 2010) . The ideal cheese using PF 3.12, Conc. of pure protease 0.01% (v/v) and 0.6/3 months ripened at 10 °C and commercial Cheddar cheese were analysed for fragrance volatiles by the SPME-GC-MS technique.
6
Quantitative LC-UV Analysis of Pantoprazole
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LC-UV analysis was carried out on a JASCO HPLC system (JASCO PU-2089 Plus binary gradient pump, AS-4050 autosampler, MD-2010 Plus diode array detector and column oven [CO2065 plus]). The software used for the operation of the equipment and data processing was ChromNAV. UV detection was performed at 288 nm. Stock solutions of 1-mg/mL pantoprazole were prepared in MeOH and were further diluted with the same solvent. An injection volume of 10 μL was used, and three parallel measurements were performed in all cases. Other chromatographic conditions including validation processes are given in Section 3.
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