Product d12492

Male C57BL/6 mice (6 wk, 18-22 g), purchased from B&K Laboratory Animal Corp., Ltd. (Shanghai, China) were randomly distributed into four treatment groups: Standard chow diet (SCD) + vehicle, SCD + LB100 1.5 mg/kg, HFD + vehicle, HFD + LB100 1.5 mg/kg. The mice were housed in a specific pathogen-free environment (24-26°C, relative humidity: 50%-60%) with a 12-h light/dark cycle and free access to food and water. For 10 wk, the mice were fed either a SCD or a HFD (60% of kilocalories as fat; Product D12492, Research Diets, New Brunswick, NJ, United States). The animals were then injected intraperitoneally with the vehicle or LB100 (three times a week) dissolved in normal saline for the next 6 wk. Blood and tissue samples were stored at -80°C. All animal experiments were performed according to the guidelines approved by the Animal Care and Use Committee of the First Affiliated Hospital, College of Medicine, Zhejiang University (Permit number: 2018-842).

We purchased 8-week old male C57BL/6 mice from Jackson Laboratories (Bar Harbor, U.S.A.). The bodyweights of the experimental mice were measured to be within the range of 20–25 g. The mice were allocated into four groups (10 mice in each group): normal chow (NC) control group; phoenixin 14 treatment group; high-fat diet (HFD) group; HFD + phoenixin 14 group. The mice were housed in a pathogen-free environment with a 12-h light/dark cycle and free access to food and water. Starting at the age of 10 weeks, the mice were fed with either NC (10% of kilocalories fat) or HFD (60% of kilocalories fat; Product D12492, Research Diets, New Brunswick, United States).13 (link),14 (link) At the same time, two groups of mice were given phoenixin 14 (100 ng/g body weight) daily by gastrogavage for a duration of ten weeks. At the end of the experiment, the mice were sacrificed and the body weights were recorded. To record the liver weight, the fat pad was removed from the liver. To harvest the organs, the bodies of mice were perfused with PBS, and the liver tissues were collected for the different experiments. All animal experiments were performed according to the guidelines approved by the Animal Care and Use Committee of Guilin People’s Hospital (GLH-AEA-20170023).