Costar ultra low attachment tissue culture plates

Manufactured by Corning

Costar Ultra Low Attachment tissue culture plates are designed for the growth and maintenance of cells in a suspension culture. These plates feature a specialized surface treatment that minimizes cell attachment, promoting the formation of spheroid structures. This product is suitable for a variety of cell types and applications that require a non-adherent, three-dimensional cell culture environment.

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Lab products found in correlation

Mammocult basal medium human, by STEMCELL (2 mentions) Proliferation supplement, by STEMCELL (1 mentions)

2 protocols using costar ultra low attachment tissue culture plates

Mammosphere Formation Assay Protocol

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Single cell dissociation for mammosphere formation assays was performed following established protocols with MDA-MB-231 and MDA-MB-436 cells plated at a density of 1×10 ⁴ cells/mL [28 (link)]. Tumorspheres were cultured in MammoCult Human Basal Medium with added Proliferation Supplement (StemCell Technologies, #05621 & #05622) on Costar Ultra Low Attachment tissue culture plates (Corning, #3471). At the end of seven days, for both primary and secondary generations, mammosphere sizes and numbers were determined using a Leica inverted microscope. Size was measured as the widest diameter with the scale bar. All experiments were performed in triplicate.

Huang W., Martin E.E., Burman B., Gonzalez M.E, & Kleer C.G. (2016). The matricellular protein CCN6 (WISP3) decreases Notch1 and suppresses breast cancer initiating cells. Oncotarget, 7(18), 25180-25193.

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Mammosphere Formation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mammosphere formation assays were performed following established protocols with single cultures and co-cultures as indicated in the figures. Cells were plated at a density of 1×10⁴ cells/mL in a 1:1 ratio (Dontu et al., 2003 (link)). Mammospheres were cultured in MammoCult Human Basal Medium with added Proliferation Supplement (StemCell Technologies, #05621 & #05622) on Costar Ultra Low Attachment tissue culture plates (Corning, #3471). At the end of 7 days for primary and secondary generations, mammosphere sizes and numbers were determined using a Leica inverted microscope. Size was measured as the widest diameter with the scale bar.

Chen Y.C., Gonzalez M.E., Burman B., Zhao X., Anwar T., Tran M., Medhora N., Hiziroglu A.B., Lee W., Cheng Y.H., Choi Y., Yoon E, & Kleer C.G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell reports, 27(13), 3916-3926.e5.

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