Penicillin/Streptomycin 100X solution and Dulbecco’s Modified Eagle Medium (DMEM) were obtained from Mediatech (Manassas, VA, USA). Fetal bovine serum (FBS) and cosmic calf serum (CCS) were purchased from Hyclone (Logan, Utah, USA). The ProFection® Mammalian Transfection System kit for calcium phosphate transfections and the trypsin used in enzymatic digests prior to LC-MS/MS analysis were from Promega (Madison, WI, USA). Protein G resin was obtained from G-Biosciences (St. Louis, MO, USA). Protein A resin and enhanced chemiluminescence (ECL) reagents were purchased from Pierce (Rockford, IL, USA), and x-ray film was from Denville Scientific (Metuchen, NJ, USA). Packing material used for HPLC was 5 μm C18-coated silica beads, 200 Å pore size, purchased from Michrom Bioresources Inc. (Auburn, CA, USA). Nitrocellulose membranes were from GVS Life Sciences (Sanford, ME, USA). All additional reagents were purchased from Sigma (St. Louis, MO, USA) unless otherwise noted.
Protein g resin
Manufactured by G Biosciences
Sourced in United States
Protein G resin is a solid-phase chromatography media used for the purification of antibodies. It consists of recombinant Protein G immobilized on agarose beads. Protein G has a high affinity for the Fc region of most immunoglobulin classes and subclasses, allowing for the capture and purification of antibodies from complex samples.
Automatically generated - may contain errors
2 protocols using protein g resin
1
Mammalian Cell Culture Reagents
2
SILAC Reagents and Materials
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Penicillin/Streptomycin 100 × solution and Dulbecco's Modified Eagle Medium (DMEM) were obtained from Mediatech (Manassas, VA). DMEM for stable isotope labeling of amino acids in cell culture (SILAC) and kanamycin sulfate were acquired from Thermo Scientific (Waltham, MA), and fetal bovine serum (FBS), dialyzed FBS for SILAC experiments, and cosmic calf serum (CCS) were purchased from Hyclone (Logan, Utah). Heavy labeled l -arginine (13C6, 15N4) and l -lysine (13C6, 15N2) were obtained from Cambridge Isotope Laboratories (Tewksbury, MA), and unlabeled amino acids were purchased from MP Biomedicals (Santa Ana, CA). The sequencing-grade trypsin used in enzymatic digests before LC–MS/MS analysis was from Promega (Madison, WI). The BSA standard for Bradford assays and the Bradford Reagent were obtained from Amresco Life Sciences, LLC. (Cleveland, OH). Protein G resin was obtained from G-Biosciences (St. Louis, MO). Protein A resin and enhanced chemiluminescence (ECL) reagents were purchased from Pierce (Rockford, IL), and x-ray film was from Denville scientific (Metuchen, NJ). Packing material used for HPLC was purchased from Michrom Bioresources Inc. (Auburn, CA). Nitrocellulose membranes were from GVS Life Sciences (Sanford, ME). All additional reagents were purchased from Sigma (St. Louis, MO) unless otherwise noted.
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