Mouse anti rabbit igg light chain hrp

The following antibodies were used: anti-EHD1 (109311, Abcam), affinity-purified rabbit polyclonal peptide antibody directed against the C-terminus of CORO2A (RELLTQREVQAKQLELEIKN; LifeTein for IP and immunoblotting), anti-TOM20 (sc-11415, Santa Cruz Biotechnology), anti-HA (T501, SAB), anti-FLAG (F1804, Sigma for immunofluorescence; ab205606, Abcam for immunoblotting), anti-GST-HRP (A01380, GenScript), anti-GAPDH-HRP (HRP-60004, Proteintech), anti-MHC-1 (purified W6/32, Leinco Technologies), anti-CD98 (315602, BioLegend), anti-CD59 (A4-233-C100, Exbio), anti-EEA1 (3288, Cell Signaling), anti-MICAL-L1 (H00085377-B01P, Novus), donkey anti-mouse-HRP (715-035-151, Jackson), mouse anti-rabbit IgG light chain-HRP (211-032-171, Jackson), Alexa Fluor 568–conjugated goat anti-rabbit (A11036, Molecular Probes), Alexa Fluor 568–conjugated donkey anti-mouse (21043, Molecular Probes), Alexa Fluor 488–conjugated goat anti-rabbit (A11034, Molecular Probes), and Alexa Fluor 488–conjugated donkey anti-mouse (A21202, Molecular Probes). The following reagents were used: CF-568–conjugated Phalloidin (44-T VWR, Biotium), cytochalasin D (Calbiochem), Alexa fluor 568–conjugated transferrin (Molecular Probes), Rhodamine-tagged EGF(Invitrogen), and Blasticidin S HCl (ThermoFisher Scientific).

The following antibodies were used (also see Table 1): Rabbit anti‐EHD1 (Abcam, ab109311), rabbit anti‐EHD4,²⁹ Rabbit anti‐acetyl‐α‐tubulin (Lys40) (D20G3) (Cell Signaling, 5335), mouse anti‐acetylated tubulin (Sigma‐Aldrich, T7451), rabbit anti‐CP110 (ProteinTech, 12780‐1‐AP), rabbit anti‐ARL13B (ProteinTech, 17711‐1‐AP), mouse anti‐GFP (Roche, 11814460001), mouse anti‐pan actin (Novus, NB600‐535), mouse anti‐GAPDH‐HRP (ProteinTech, HRP‐60004), donkey anti‐mouse‐HRP (Jackson, 715‐035‐151), mouse anti‐rabbit IgG light chain‐HRP (Jackson, 211‐032‐171), DAPI (4′,6‐diamidino‐2‐phenylindole, dihydrochloride) (Molecular Probes, D1306), biotin‐conjugated goat anti‐GFP (Rockland, 600‐106‐215), Alexa‐fluor 488‐conjugated streptavidin (Molecular Probes, S11223), Alexa‐fluor 488‐conjugated goat anti‐mouse (Molecular Probes, A11029), Alexa‐fluor 568‐conjugated goat anti‐rabbit (Molecular Probes, A11036), Alexa‐fluor 568‐conjugated goat anti‐mouse (Molecular Probes, A11031), Alexa‐fluor 633‐conjugated goat anti‐rabbit (Molecular Probes, A21070).