HIV p24 antigen and CATB concentration were measured from macrophage supernatants at 11 days post-infection by ELISA as described in previous studies (Rodríguez-Franco et al., 2012 (link)). To assess HIV-1 infection levels in macrophages after drug treatments, an HIV p24 antigen ELISA was performed according to the manufacturer’s instructions (Express BioTech, Maryland, USA). CATB secretion was determined at 3, 6, 9 and 11 days post-infection from cell culture supernatants using the Human Pro-Cathepsin B Quantikine ELISA kit (R&D Systems, Minnesota, USA). Total Human Cathepsin B Quantikine ELISA kit was also used (R&D Systems, Minnesota, USA). Macrophage supernatants from 11 days post-infection were analyzed for CATB in a Versa Fluor Fluorometer (Bio-Rad) (Bio Vision, San Francisco, CA) that contains 400 nm excitation and 505 nm emission filters.
Human pro cathepsin b quantikine elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human Pro-Cathepsin B Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of pro-Cathepsin B levels in cell culture supernates, serum, and plasma.
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Lab products found in correlation
2 protocols using human pro cathepsin b quantikine elisa kit
1
Quantifying HIV-1 Infection and Cathepsin B Levels
2
HIV Infection Measurement in Macrophages
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HIV p24 antigen and CATB concentration were measured from macrophage supernatants at 11 days post-infection by ELISA as described in previous studies (Rodríguez-Franco et al. 2012 (link)). To assess HIV-1 infection levels in macrophages after drug treatments, an HIV p24 antigen ELISA was performed according to the manufacturer’s instructions (Express BioTech, Maryland, USA). CATB secretion was determined at 3, 6, 9 and 11 days post-infection from cell culture supernatants using the Human Pro-Cathepsin B Quantikine ELISA kit (R&D Systems, Minnesota, USA). Total Human Cathepsin B Quantikine ELISA kit was also used (R&D Systems, Minnesota, USA). Macrophage supernatants from 11 days post-infection were analyzed for CATB in a Versa Fluor Fluorometer (Bio-Rad) (Bio Vision, San Francisco, CA) that contains 400 nm excitation and 505 nm emission filters.
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