At the end of the experiment with continuous supply of test substance for 84 days, blood from vena cava of each mouse was collected under inhalation anesthesia with 3% isoflurane for induction and 1.5–1.8% isoflurane for maintenance of anesthesia (in the mixture of 28.5% O2 and 70% N2O; Hana Pharm. Co., Hwasung, Korea) using rodent inhalation anesthesia apparatus (Surgivet, Waukesha, WI, USA) and rodent ventilator (Harvard Apparatus, Cambridge, UK). The blood samples were stored in NaF glucose vacuum tubes (Becton Dickinson, Franklin Lakes, NJ, USA), and plasma was separated and stored in an ultra-deep freezer (Sanyo, Tokyo, Japan) at −150 °C until further analysis. An automated blood analyzer (Fuji Medical System Co., Ltd., Tokyo, Japan) was used to measure blood glucose levels.
Naf glucose vacuum tubes
Manufactured by BD
Sourced in United States
NaF glucose vacuum tubes are laboratory equipment used to collect and preserve blood samples for the measurement of glucose levels. These tubes contain sodium fluoride (NaF) as a preservative, which helps to inhibit the breakdown of glucose in the sample.
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2 protocols using naf glucose vacuum tubes
1
Chronic Mouse Blood Glucose Measurement
2
Measurement of Serum Biochemical Markers
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For the measurement of blood glucose levels, blood samples were collected from the caudal vena cava into NaF glucose vacuum tubes (Becton-Dickinson, Franklin Lakes, NJ, USA) and plasma was separated at sacrifice. Blood glucose levels were measured using an automated blood glucose analyzer (200 FR; Toshiba, Tokyo, Japan). For other serum biochemistry measurements, blood samples were collected from the caudal vena cava into clotting activated serum tubes. The samples were centrifuged at 16,200 × g for 10 min at ambient temperature. The alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), blood urine nitrogen (BUN), TG, total cholesterol (TC), creatinine, low-density lipoprotein (LDL), high-density lipoprotein (HDL) cholesterol and cholesterol levels were measured using an automated blood analyzer (Hemagen Analyst Hemagen Diagnostic, Columbia, MD, USA), while serum LDL and HDL levels were detected using different blood analyzer (AU400; Olympus, Tokyo, Japan). Serum insulin and blood HbA1c levels were measured using a HbA1c measuring system (Infopia, Anyang, Korea) and an enzyme-linked immunosorbent assay (ELISA) kit (Alpco Diagnostics, Windham, NH, USA), as previously described (27 (link),28 (link)).
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