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Ma1 310

Manufactured by Thermo Fisher Scientific
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Sourced in Germany

The MA1-310 is a laboratory instrument designed for sample preparation and analysis. It features precise temperature control and stirring capabilities to facilitate various experimental protocols. The core function of the MA1-310 is to provide a controlled environment for sample handling and processing.

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Lab products found in correlation

Ac 40, by Merck Group (2 mentions) Sc 816, by Santa Cruz Biotechnology (2 mentions) Sc 166394, by Santa Cruz Biotechnology (2 mentions) Sc 67060, by Santa Cruz Biotechnology (2 mentions) Sc 20681, by Santa Cruz Biotechnology (2 mentions) Ma5 13065, by Thermo Fisher Scientific (2 mentions) Sc 32233, by Santa Cruz Biotechnology (2 mentions) Mcf10a cells, by American Type Culture Collection (1 mentions) Anti β actin monoclonal antibody a5441, by Merck Group (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) H 300, by Santa Cruz Biotechnology (1 mentions) Rabbit igg isotype control, by Novus Biologicals (1 mentions) Sc 8974, by Santa Cruz Biotechnology (1 mentions) H 150, by Santa Cruz Biotechnology (1 mentions) Sc 48524 r, by Santa Cruz Biotechnology (1 mentions) Sc 543, by Santa Cruz Biotechnology (1 mentions) Hc 20, by Santa Cruz Biotechnology (1 mentions)

4 protocols using ma1 310

1

Molecular Characterization of Breast Cancer Cell Lines

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The MDA-MB231 and the MDA-MB468 breast cancer cell lines and the MCF-10A cells were purchased from ATCC. These cells were grown as described previously [18 ,19 (link)]. The anti-β-actin monoclonal antibody (A5441) was from Sigma-Aldrich, the anti-Snail antibody (SN9H2) was from Cell Signaling, the anti-EGFR antibody (610017) was from BD Biosciences, the anti CK18 antibody (M7010) was from DAKO, the anti-smooth muscle actin (MA1-26017) and the anti-estrogen receptor alpha (MA1-310) antibodies were from Thermo Scientific, and the anti-MMP2 (MAB3308) and the anti-MMP9 (AB13458) antibodies were from Millipore. The anti-SHP2 (SC-7384), the anti-vimentin (SC-32322), the anti-progesterone receptor (SC-538), and the anti-fibronectin (SC-18825) antibodies were from Santa Cruz Biotechnology. Anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase were purchased from Jackson Immuno-Research Laboratories.
Zhao H, & Agazie Y.M. (2015). Inhibition of SHP2 in basal-like and triple-negative breast cells induces basal-to-luminal transition, hormone dependency, and sensitivity to anti-hormone treatment. BMC Cancer, 15, 109.
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2

Protein Expression and Interaction Analysis

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20 µg of protein aliquots from cell lysates of testis from V7BAC transgenic mice were separated by 15% SDS-PAGE and transferred to nitrocellulose membranes. The membranes were probed with primary antibodies against VAMP7 (sc-166394, 1:100, Santa-Cruz or sc-67060, 1:50, Santa-Cruz), AR (sc-816, 1:1,000, Santa-Cruz), ESR1 (MA1-310, 1:200, Thermo-Fisher), LMNA (sc-20681, 1:200, Santa-Cruz), GAPDH (sc-32233, 1:200, Santa-Cruz), and ACTB (AC-40, 1:5,000, Sigma). HeLa cells were cotransfected with AR or ESR1 or VAMP7 or in combination. After 24 h of overexpression, cell lysates were prepared and immunoprecipitation was carried out using antibodies raised against AR (sc-816, Santa-Cruz) or ESR1 (MA5-13065, Thermo-Fisher) or VAMP7 (sc-67060, Santa-Cruz) or control IgG antibody. The samples were later subjected to western blotting with the indicated antibodies. Input was 5% of the lysates.
Tannour-Louet M., Han S., Louet J.F., Zhang B., Romero K., Addai J., Sahin A., Cheung S.W, & Lamb D.J. (2014). Increased gene copy number of the vesicle SNARE VAMP7 disrupts human male urogenital development through altered estrogen action. Nature medicine, 20(7), 715-724.
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3

Protein Expression and Interaction Analysis

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20 µg of protein aliquots from cell lysates of testis from V7BAC transgenic mice were separated by 15% SDS-PAGE and transferred to nitrocellulose membranes. The membranes were probed with primary antibodies against VAMP7 (sc-166394, 1:100, Santa-Cruz or sc-67060, 1:50, Santa-Cruz), AR (sc-816, 1:1,000, Santa-Cruz), ESR1 (MA1-310, 1:200, Thermo-Fisher), LMNA (sc-20681, 1:200, Santa-Cruz), GAPDH (sc-32233, 1:200, Santa-Cruz), and ACTB (AC-40, 1:5,000, Sigma). HeLa cells were cotransfected with AR or ESR1 or VAMP7 or in combination. After 24 h of overexpression, cell lysates were prepared and immunoprecipitation was carried out using antibodies raised against AR (sc-816, Santa-Cruz) or ESR1 (MA5-13065, Thermo-Fisher) or VAMP7 (sc-67060, Santa-Cruz) or control IgG antibody. The samples were later subjected to western blotting with the indicated antibodies. Input was 5% of the lysates.
Tannour-Louet M., Han S., Louet J.F., Zhang B., Romero K., Addai J., Sahin A., Cheung S.W, & Lamb D.J. (2014). Increased gene copy number of the vesicle SNARE VAMP7 disrupts human male urogenital development through altered estrogen action. Nature medicine, 20(7), 715-724.
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4

Antibody Characterization for ESR1, ESR2, and GPER1

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The antibodies against ESR1 were rabbit polyclonal antibody HC-20 (against the C-terminus of the human protein) (sc-543, Santa Cruz Biotechnology, Inc. Heidelberg, Germany) and mouse monoclonal antibody MA1-310 (against the synthetic peptide within the DNA-binding domain of the human protein) (Thermo Fisher Scientific, IL, USA). The antibodies against ESR2 were rabbit polyclonal antibody H-150 (against amino acids 1–150 of the human protein) (sc-8974, Santa Cruz Biotechnology, Inc. Heidelberg, Germany) and mouse monoclonal antibody 6A12 MA1-23221 (against amino acids 1–153 of the human protein) (Thermo Fisher Scientific, IL, USA). The antibodies against GPER1 were rabbit polyclonal antibody K-19 (against the internal region of human GPR30) (sc-48524-R, Santa Cruz Biotechnology, Inc. Heidelberg, Germany) and rabbit polyclonal antibody H-300 (against amino acids 76–375 of human GPR30) (sc-134576, Santa Cruz Biotechnology, Inc. Heidelberg, Germany). The controls used in the experiments were rabbit IgG isotype control (Novus Biological, Centennial, CO, USA) and mouse IgG1/IgG2 isotype control (EXBIO, Vestec, Czech Republic).
Antalikova J., Secova P., Horovska L., Krejcirova R., Simonik O., Jankovicova J., Bartokova M., Tumova L, & Manaskova-Postlerova P. (2020). Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?. Cells, 9(1), 183.
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