Anti vinculin v4505

Manufactured by Merck Group

Anti-vinculin (V4505) is a monoclonal antibody used for the detection and quantification of vinculin, a cytoskeletal protein involved in cell-cell and cell-matrix adhesion. This antibody can be used in various applications such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of vinculin in different cell types and tissues.

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Lab products found in correlation

Ab32034, by Abcam (1 mentions) Plx 4720, by MedChemExpress (1 mentions) Anti β tubulin t4026, by Merck Group (1 mentions) Otx 008, by Axon Medchem (1 mentions) Ab193355, by Abcam (1 mentions) Anti vsv g clone p5d4, by Merck Group (1 mentions) Anti phospho s6 ribosomal protein ser240 244 2215, by Cell Signaling Technology (1 mentions) Anti his, by Abcam (1 mentions) Anti p27 kip1 antibody, by Cell Signaling Technology (1 mentions) Anti flag, by Merck Group (1 mentions) N cadherin, by Santa Cruz Biotechnology (1 mentions) Anti e cadherin, by BD (1 mentions) Anti tgf r1 antibody, by Abcam (1 mentions) Sb431542 hydrate, by Merck Group (1 mentions) Plexin b2, by Santa Cruz Biotechnology (1 mentions)

4 protocols using anti vinculin v4505

Antibody Detection of EGFR and p27/Kip1

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EGFR was detected with an antibody purchased from Enzo Life Sciences, Farmingdale, NY, USA (cat. ALX-804-064-C100). The p27/Kip1 antibody (ab32034) was purchased from Abcam, Cambridge, UK. Other antibodies applied in this study were anti-vinculin (V4505, Sigma) and anti-βtubulin (T4026, Sigma). Secondary antibodies were purchased from Promega (Madison, WI, USA) or Jackson Laboratories (Bar Harbor, ME, USA). PLX-4720 was purchased from Med Chem Express (Monmouth Junction, NJ, USA) and resuspended in DMSO as the vehicle. OTX-008 was purchased from Axon Medchem (Reston, VA, USA). Purified Galectin-1 (cat: 10290-HNAE) was purchased from Sino Biological Europe GmbH (Eschborn, Germany).

Rizzolio S., Corso S., Giordano S, & Tamagnone L. (2020). Autocrine Signaling of NRP1 Ligand Galectin-1 Elicits Resistance to BRAF-Targeted Therapy in Melanoma Cells. Cancers, 12(8), 2218.

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Western Blotting Analysis of EGFR Signaling

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Anti‐PlexinB2 antibody used for Western blotting analysis was purchased from Abcam (ab193355; dilution 1:500). EGFR antibody was provided by Enzo Life Sciences (ALX‐804‐064‐C100; dil. 1:500), while anti‐phospho‐EGFR‐specific antibodies (directed to p‐Tyr1068; ab5644; dil. 1:500) were from Abcam. Anti‐vinculin (V4505; dil. 1:1,000) and anti‐VSV‐G (clone P5D4; dil. 1:1,000) were provided by Sigma‐Aldrich. Anti‐phospho‐p44/42‐MAPK (Erk1/2; Thr202/Tyr204; #4370; dil. 1:1,000), anti‐p44/42‐MAPK (Erk1/2; 137F5; #4695; dil. 1:1,000), and anti‐phospho‐S6 ribosomal protein (Ser240/244; #2215; dil. 1:1,000) antibodies were from Cell Signaling.

Brundu S., Napolitano V., Franzolin G., Lo Cascio E., Mastrantonio R., Sardo G., Cascardi E., Verginelli F., Sarnataro S., Gambardella G., Pisacane A., Arcovito A., Boccaccio C., Comoglio P.M., Giraudo E, & Tamagnone L. (2023). Mutated axon guidance gene PLXNB2 sustains growth and invasiveness of stem cells isolated from cancers of unknown primary. EMBO Molecular Medicine, 15(3), e16104.

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ChIP Assay for Protein Targets

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Anti-YAP antibody ChIP (Abcam, ab52771), Anti-YY1 antibody-ChIP Grade (Abcam, ab38422). Anti-EZH2 ChIP (Active motif, #39901). Anti-Lamin A/C (sc-6215 (N-18), 1:500). Anti-tubulin (T5168) anti-actin (A4700) and anti-vinculin (V4505) were from Sigma. Anti-YAP Cell Signaling (#4912). Anti-p27 Kip1 antibody (Cell signaling, 2552S). Anti-Flag (Sigma, F1804–200UG). Anti-His (Abcam, #18184). Anti-TAZ (D3I6D) (Cell signaling, 70148).

Hoxha S., Shepard A., Troutman S., Diao H., Doherty J.R., Janiszewska M., Witwicki R.M., Pipkin M.E., Ja W.W., Kareta M.S, & Kissil J.L. (2020). YAP-mediated recruitment of YY1 and EZH2 represses transcription of key cell cycle regulators.. Cancer research, 80(12), 2512-2522.

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Comprehensive Cell Line and Antibody Protocol

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MDA-MB231 and PC-3 tumor cell lines and COS-7 immortalized cells were obtained from the American Type Culture Collection and cultured in a humidified incubator with 5% CO 2 at 37°C in Dulbecco's modified Eagle's medium or RPMI 1640 media supplemented with 10% fetal bovine serum (FBS), penicillin, and streptomycin, respectively. Antibodies directed against Sema4C (sc-136445), N-cadherin (sc-7939), fibronectin (sc-8422), Zeb1 (sc-25388), SMAD1 (sc-7965), ID1 (sc-488), actin (sc-1616), PlexinB2 (sc-34504), TGF--R2 (sc-17792), and ACTR1/ACVR1 (sc-374523) were all from Santa Cruz Biotechnology. Anti-vimentin (VIM-3B4) was from Merck. Anti-p-SMAD1/5/9 (D5B10, no. 13820) and anti-p-SMAD2/3 (D27F4, no. 8828) were from Cell Signaling Technology. Anti-vinculin (V4505) and anti-Flag (clone M2_F3165) were from Sigma-Aldrich. Anti-E-cadherin was from BD Transduction Laboratories (catalog no. 610182). Anti-TGF--R1 antibody was from Abcam (ab31013). Anti-BMPR1A was purchased from Proteintech (catalog no. 12702-1-AP). Cell treatments with 1 to 5 M SB-431542 hydrate (Sigma-Aldrich) were performed for 48 to 72 hours, as described. Purified recombinant Sema4C ectodomain (catalog no. AF6125) was purchased from R&D Systems. A tumor multiple tissue array containing primary and metastatic breast cancer samples (BR1008A) was obtained from US Biomax Inc. (Rockville, MD).

Gurrapu S., Franzolin G., Fard D., Accardo M., Medico E., Sarotto I., Sapino A., Isella C, & Tamagnone L. (2019). Reverse signaling by semaphorin 4C elicits SMAD1/5- and ID1/3-dependent invasive reprogramming in cancer cells. Science signaling, 12(595).

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