Chrysanthemum zawadskii, Peppermint (Mentha piperita) and Glycyrrhiza glabra were purchased from Kyeondong market. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin antibiotics were purchased from Gibco; Thermo Fisher Scientific, Inc. Griess reagent and LPS (L2630) were procured Sigma-Aldrich; Merck KGaA. Quanti-Max™ WST-8 Cell Viability Assay Kit has gotten from Biomax. Goat anti-mouse IgG (H+L) Alexa Fluor plus 488 conjugated secondary antibodies were purchased from Invitrogen; Thermo Fisher Scientific, Inc. Enzyme-linked immunosorbent assay (ELISA) kit PGE2, TNF-α, IL-6, and IL-1β were procured from R&D System. Mouse IFN Beta ELISA Kit (TCM, Serum) was supplied by BL Assay Science. HO-1 activity kit was purchased from Cusabio. Radio-immunoprecipitation assay buffer (RIPA buffer) came from Thermo Fisher Scientific, Inc. Bradford's assay reagent was purchased from Bio-Rad Laboratories, Inc. 5X SDS-PAGE loading buffer was purchased from Biosesang. Antibodies against iNOS, COX-2, p-NF-κB, p-IκB, p-Akt, p-STAT1, STAT1, and β-actin were purchased from Santa Cruz Biotechnology, Inc. HO-1 antibody was procured from Abcam. Horseradish peroxidase (HRP)-IgG secondary antibodies and diamidino-2-phenylindole (DAPI) were purchased from Cell Signaling Technology, Inc.
Diamidino 2 phenylindole dapi
Manufactured by Cell Signaling Technology
Sourced in United States
Diamidino-2-phenylindole (DAPI) is a fluorescent stain that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a counterstain in fluorescence microscopy to visualize cell nuclei.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Ho 1 antibody, by Abcam (1 mentions)
Lps l2630, by Merck Group (1 mentions)
P nf κb, by Santa Cruz Biotechnology (1 mentions)
P stat1, by Santa Cruz Biotechnology (1 mentions)
Penicillin streptomycin antibiotic, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
P akt, by Santa Cruz Biotechnology (1 mentions)
Bradford s assay reagent, by Bio-Rad (1 mentions)
Sds page loading buffer, by Biosesang (1 mentions)
Quanti max wst 8 cell viability assay kit, by Tissue Array (1 mentions)
Radio immunoprecipitation assay buffer ripa buffer, by Thermo Fisher Scientific (1 mentions)
P iκb, by Santa Cruz Biotechnology (1 mentions)
Cox 2, by Santa Cruz Biotechnology (1 mentions)
Griess reagent, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Anti trpm7, by Abcam (1 mentions)
Mmp 2 d8n9y rabbit mab, by Cell Signaling Technology (1 mentions)
Accuprime taq dna polymerase system, by Thermo Fisher Scientific (1 mentions)
Cleaved caspase 3 asp175 antibody, by Cell Signaling Technology (1 mentions)
2 protocols using diamidino 2 phenylindole dapi
1
Anti-inflammatory Activity of Plant Extracts
2
Western Blot Analysis of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SuperScript® III First-Strand Synthesis System and AccuPrime™ Taq DNA Polymerase System were purchased from Invitrogen/Life Technologies Corporation, USA. Anti-TRPM7 (cat#ab85016), anti-Cofilin (phospho S3) antibody (cat#ab12866) and anti-Cofilin antibody [EP6376] (cat#ab134963) were purchased from Abcam, USA. Diamidino-2-phenylindole (DAPI, cat#4083), MMP-2 (D8N9Y) rabbit mAb (cat#13132), cleaved caspase-3 (Asp175) antibody (cat#9661), phosphor-Akt (ser473) antibody (p-Akt, cat#9271), Akt antibody (t-Akt, cat#9272) and phospho-p44/42 MAPK antibody (p-ERK1/2, cat#5726) were purchased from Cell Signaling Technology, USA. Anti-MAP Kinase ERK1/ERK2 Rabbit pAb (t-ERK1/2, cat#442704) was purchased from Millipore. Pierce™ BCA Protein Assay Kit was product of Pierce Biotechnology, USA. All cell culture materials were purchased from Gibco Life Technologies Corporation (USA). Sodium Dodecyl Sulfate (SDS), sodium chloride (NaCl) and phenylmethylsulfonyl fluoride (PMSF) were purchased from Bioshop, Canada. All other reagents used were obtained from Sigma-Aldrich, USA unless mentioned otherwise.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!