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Biomark x ray

Manufactured by Carestream
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The Biomark X-ray is a piece of lab equipment designed for X-ray imaging. It is used to capture high-quality images for analysis and diagnostic purposes.

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Lab products found in correlation

Anti α tubulin, by Bio-Rad (2 mentions) Nupage sample loading buffer, by Thermo Fisher Scientific (2 mentions) Anti flag m2, by Merck Group (2 mentions) Anti cdk1, by Santa Cruz Biotechnology (2 mentions) Anti cdc25b, by Cell Signaling Technology (2 mentions) Anti pkcβ2 c terminal, by Santa Cruz Biotechnology (2 mentions) Anti pt641 pkcα β2, by Cell Signaling Technology (2 mentions) Super rx n, by Fujifilm (2 mentions) Ecl select, by GE Healthcare (2 mentions) Anti erk2, by Santa Cruz Biotechnology (2 mentions)

2 protocols using biomark x ray

1

Immunoblotting Protein Detection Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
All samples were mixed with NuPAGE sample loading buffer (Invitrogen) and heated at 95°C for 5 min. Antibodies used for western blotting were anti-FLAG M2 (mouse, Sigma), anti-ERK2 (rabbit, Santa Cruz), anti-α-tubulin (rat, AbD Serotec), anti-PKCβ2 C-terminal (rabbit, Santa Cruz), anti-CDK1 (mouse, Santa Cruz), anti-CDC25B (rabbit, Cell Signaling Technology), and anti-pT641 PKCα/β2 (rabbit, Cell Signaling Technology). All primary antibodies were used in 5% dry milk in PBS with 0.1% Tween 20 and incubated overnight at 4°C. Horseradish peroxidase-coupled secondary anti-mouse (Dako), anti-rabbit (Fisher), and anti-rat (Santa Cruz) antibodies were detected by enhanced chemiluminescence (ECL select, GE Healthcare) and Biomark X-ray (Carestream) or Super RX-N (Fujifilm) films.
Tischer T, & Schuh M. (2016). The Phosphatase Dusp7 Drives Meiotic Resumption and Chromosome Alignment in Mouse Oocytes. Cell reports, 17(5), 1426-1437.
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2

Immunoblotting Protein Detection Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
All samples were mixed with NuPAGE sample loading buffer (Invitrogen) and heated at 95°C for 5 min. Antibodies used for western blotting were anti-FLAG M2 (mouse, Sigma), anti-ERK2 (rabbit, Santa Cruz), anti-α-tubulin (rat, AbD Serotec), anti-PKCβ2 C-terminal (rabbit, Santa Cruz), anti-CDK1 (mouse, Santa Cruz), anti-CDC25B (rabbit, Cell Signaling Technology), and anti-pT641 PKCα/β2 (rabbit, Cell Signaling Technology). All primary antibodies were used in 5% dry milk in PBS with 0.1% Tween 20 and incubated overnight at 4°C. Horseradish peroxidase-coupled secondary anti-mouse (Dako), anti-rabbit (Fisher), and anti-rat (Santa Cruz) antibodies were detected by enhanced chemiluminescence (ECL select, GE Healthcare) and Biomark X-ray (Carestream) or Super RX-N (Fujifilm) films.
Tischer T, & Schuh M. (2016). The Phosphatase Dusp7 Drives Meiotic Resumption and Chromosome Alignment in Mouse Oocytes. Cell reports, 17(5), 1426-1437.
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