Bs 2442r

Manufactured by Bioss Antibodies

Sourced in China, United States, United Kingdom

The BS-2442R is a centrifuge instrument designed for general laboratory applications. It features a compact design, variable speed control, and a high-quality rotor for efficient separation of samples. The centrifuge operates at a maximum speed of 4,000 RPM and can accommodate a range of sample tube sizes. This product is intended for use in various laboratory settings to facilitate sample preparation and processing tasks.

Automatically generated - may contain errors

Lab products found in correlation

Bs 3550r, by Bioss Antibodies (3 mentions) Ab 528428, by Abcam (2 mentions) Bs 20702r, by Bioss Antibodies (2 mentions) Wf10x, by Olympus (2 mentions) Triton x 100, by Merck Group (1 mentions) Goat anti mouse igg h l a0216, by Beyotime (1 mentions) Goat anti rabbit igg hrp, by Bioss Antibodies (1 mentions) Ab8226, by Abcam (1 mentions) Ab59493, by Abcam (1 mentions) Bs 4509r, by Bioss Antibodies (1 mentions) Bs 1630r, by Bioss Antibodies (1 mentions) Bs 0295g, by Bioss Antibodies (1 mentions) Bs 1402r, by Bioss Antibodies (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

3 protocols using bs 2442r

Immunofluorescence Analysis of Muscle Stem Cells

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BSC were grown on 14 mm coverslip-bottomed dishes and fixed with 4%
paraformaldehyde at ambient temperature for 20 min, washed with phosphate
buffered saline (PBS), permeabilized with 0.2% Triton X-100 (Sigma-Aldrich) at
ambient temperature for 30 min, and washed with PBS again. The polyclonal
anti-myogenic differentiation 1 (MYOD1) (BS-2442R, Bioss, Beijing, China),
anti-paired box 7 (Pax7) (AB-528428, Abcam, Cambridge, UK), and anti-desmin Po
(BS-20702R, Bioss) were diluted in PBS with 5% bovine serum albumin (BSA) at a
ratio of 1:100 and incubated at ambient temperature for 2 h. The horseradish
peroxidase (HRP)-labeled Goat Anti-Mouse Immunoglobulin G (IgG) (H + L) (A0216,
Beyotime, Shanghai, China) or Goat Anti-Rabbit IgG-HRP antibody (BS-3550R,
Bioss) was diluted in PBS with 5% BSA at a ratio of 1:200 and incubated for 2 h
at ambient temperature in the dark. Finally, the BSC was incubated in the 0.1
μg/mL 4’,6-diamidino-2-phenylindole, dihydrochloride solution for
30 min in the dark and observed with a fluorescence microscope (WF10X, Olympus,
Tokyo, Japan).

Zhang J., Li Q., Yan Y., Sun B., Wang Y., Tang L., Wang E., Yu J., Nogoy K.M., Li X, & Choi S.H. (2021). Effect of ciglitazone on adipogenic transdifferentiation of bovine skeletal muscle satellite cells. Journal of Animal Science and Technology, 63(4), 934-953.

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Immunofluorescence Staining of Muscle Satellite Cells

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To verify whether the cells isolated were BSC, the primary cells were stained for
immu-nofluorescence. Cultured satellite cells were fixed with ice-cold 4%
paraformaldehyde for 20 min, rinsed twice with PBS, and permeated with 0.2%
Triton x-100 (in PBS) at room temperature for 30 min. The permeabilized cells
were blocked with PBS containing 5% bovine serum albumin (BSA) and shaken at
room temperature for 1 h, and incubated with primary antibodies: polyclonal
anti-myogenic differentiation 1 (MYOD1; BS-2442R, Bioss, Woburn, MA, USA),
anti-Pax7 (AB-528428, Abcam, Cambridge, UK), and anti-desmin Po (BS-20702R,
Bioss) in 5% BSA at 1:100 dilutions for 2 h at room temperature. After washing
with PBS, the cells were incubated with HRP-labeled Goat Anti-Mouse IgG (H + L)
(A0216, Beyotime, Shanghai, China) or Goat Anti-rabbit IgG-HRP antibody
(BS-3550R, Bioss) for 2 h at room temperature in dark room and mounted in 0.1
μg/mL DAPI solution for 30 min. The staining of the cells was observed
using a fluorescence microscope (WF10X, Olympus, Tokyo, Japan) afterwards.

Zhang J., Li Q., Nogoy K.M., Sun J., Sun B., Wang Y., Tang L., Yu J., Jin X., Li X, & Choi S.H. (2021). Effect of palmitoleic acid on the differentiation of bovine skeletal muscle satellite cells. Journal of Animal Science and Technology, 63(4), 919-933.

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Protein Expression Analysis in Transfected Cells

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Transfected cells were lysed in RIPA buffer containing 1% phenylmethylsulfonyl fluoride (PMSF). A BCA protein kit (Pierce, Thermo Fisher Scientific Inc., Waltham, MA, USA) was used to determine the total protein concentration. Protein samples were separated using 10% SDS-PAGE and transferred onto PVDF membranes (Millipore, Billerica, MA, USA). The membranes were incubated overnight at 4 °C with the following antibodies: anti-DGAT1 (ab189994, Abcam, Cambridge, UK), anti-DGAT2 (ab59493, Abcam, Cambridge, UK), anti-PPARγ (bs-4509R, Bioss, Beijing, China), anti-C/EBPα (bs-1630R, Bioss, Beijing, China), anti-SREBF1(bs-1402R, Bioss, Beijing, China), anti-Pax7 (ab61067, Abcam, Cambridge, UK), anti-MYOD (bs-2442R, Bioss, Beijing, China), and anti-MYOG (bs-3550R, Bioss, Beijing, China). Thereafter, the membranes were washed and incubated with horseradish peroxidase-conjugated goat anti-mouse or goat anti-rabbit IgG (bs-0295G; Bioss, Beijing, China) for 1 h. β-actin (ab8226, Abcam, Cambridge, UK) was used as an endogenous control. A grayscale intensity analysis was performed using ImageJ software (NIH).

Zhang J.F., Choi S.H., Li Q., Wang Y., Sun B., Tang L., Wang E.Z., Hua H, & Li X.Z. (2022). Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells. Animals : an Open Access Journal from MDPI, 12(14), 1847.

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