Lsr model 2 flow cytometer

For experiments involving purification of cell populations by flow sorting, an Influx or a FACSAria II was used (BD Biosciences, Oxford, UK). Apoptosis and cell cycle analyses were performed using an LSR Model II flow cytometer (BD Biosciences). Apoptosis was measured using an Annexin V-APC kit (BD Pharmingen, Oxford UK) according to the manufacturer’s instructions. Cell cycle analyses were performed as described.^{50 (link)} Quantitative PCR was performed as described ^{34 (link)} using Universal Probe Library System (Roche, UK) designed primers and probes (Supplementary Table 5). Antibodies for western blotting and pharmacological inhibitors are shown in Supplementary Tables 6 and 7 respectively. Lipid assays were performed as detailed elsewhere.^{51 (link),52 (link)}