Vcx 750 230 5

Manufactured by Sonics

The VCX 750-230 V is a lab equipment product that provides a core function. It operates at 230 V.

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Lab products found in correlation

Zetasizer nano z, by Malvern Panalytical (2 mentions) Orius sc1000b, by Ametek (1 mentions) Digital micrograph software, by Ametek (1 mentions) Jem 1400, by JEOL (1 mentions)

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VCX 750 (177 citations)

2 protocols using vcx 750 230 5

Characterization of Food Additive E171

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The E171 food additive was purchased as powder from the website of a French commercial supplier of food colouring agents. These food-grade TiO₂ particles were prepared following the generic Nanogenotox dispersion protocol as previously described [7 (link)]. The E171 stock suspension (25.6 mg/mL) was sonicated in an ice bath for 16 min at 30% amplitude (VCX 750-230 V, Sonics Materials) to obtain a stable dispersion of TiO₂ particles and then stocked at 4 °C during 15 days maximum before use. Dynamic light scattering (DLS, ZetaSizer nano ZS; Malvern Instruments Ltd.) measurements were performed on TiO₂ particles in ultrapure water (pH = 8.92) and perfusion medium (PM = Earle + 2% BSA, pH 7.4). Five μL of E171 stock suspension were diluted in 3 mL of PM and the hydrodynamic diameter (Z-average), polydispersity index and zeta potential of the TiO₂ particles were measured. Particle diameters were measured by scanning electron microscopy (SEM,n = 600 particles) and are expressed as the percentage of NPs by number. The specific surface area (SSA) of the particles was assessed according to the Brunauer, Emmet and Teller method (BET).

Guillard A., Gaultier E., Cartier C., Devoille L., Noireaux J., Chevalier L., Morin M., Grandin F., Lacroix M.Z., Coméra C., Cazanave A., de Place A., Gayrard V., Bach V., Chardon K., Bekhti N., Adel-Patient K., Vayssière C., Fisicaro P., Feltin N., de la Farge F., Picard-Hagen N., Lamas B, & Houdeau E. (2020). Basal Ti level in the human placenta and meconium and evidence of a materno-foetal transfer of food-grade TiO2 nanoparticles in an ex vivo placental perfusion model. Particle and Fibre Toxicology, 17, 51.

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Characterization of Food-Grade Silica Nanoparticles

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The

{SiO}_{2}

sample was purchased as powder from a French commercial supplier producing food-grade (

food-grade silicon dioxide

) precipitated amorphous silica (INS 551) in the frame of the PAIPITO project funded by the French National Research Agency.^{17 (link)} For physicochemical characterization,

food-grade silicon dioxide

was sonicated (VCX 750-230V; Sonics and Materials, Inc.) in ultrapure water (

1 milligram per milliliter

) for 1 min at 40% amplitude in an ice bath.^{24 (link)} The hydrodynamic diameter (Z-average), polydispersity index, and zeta potential were determined using dynamic light scattering (DLS) (Zetasizer Nano ZS; Malvern Instruments Ltd.) with

10 microliters

food-grade silicon dioxide

suspension diluted in

2 milliliters

of ultrapure water (Table S1).^{24 (link)} Transmission electron microscopy (TEM) analysis was performed on a JEM 1400 (JEOL) equipped with camera Orius SC1000B (Gatan, Inc.) driven by DigitalMicrograph software (Gatan-Ametek). The sonicated suspension of

food-grade silicon dioxide

was diluted in ultrapure water (

10 milligrams per milliliter

), and size measurement was determined from bright-field TEM images by using the image processing open-source software ImageJ (NIH).

Lamas B., Martins Breyner N., Malaisé Y., Wulczynski M., Galipeau H.J., Gaultier E., Cartier C., Verdu E.F, & Houdeau E. (2024). Evaluating the Effects of Chronic Oral Exposure to the Food Additive Silicon Dioxide on Oral Tolerance Induction and Food Sensitivities in Mice. Environmental Health Perspectives, 132(2), 027007.

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