For the histological examination, bone samples from the mandibular lesion site were harvested. For decalcification, after fixation, the mandibula of the animals were kept in a mix of 8% formic and 8% clorhidric acid for 24 h and embedded in paraffin. The samples were fixed in 10% buffered neutral formalin, embedded in paraffin. The sections were made with a high-precision microtome Leica RM 2125 RT, at 5-μm thick, and stained by the hematoxylin–eosin method (HE). The slides were examined under a BX51 Olympus microscope, and images were taken with an Olympus UC 30 digital camera and processed using Olympus Basic Stream software. Sections were examined by an independent observer blinded to the experimental protocol.
Uc30 digital camera
Manufactured by Olympus
Sourced in Japan
The UC30 digital camera is a compact, high-resolution imaging device designed for laboratory applications. It features a 3.0-megapixel sensor and captures images with a resolution of up to 2048 x 1536 pixels. The camera provides a direct USB connection for easy data transfer and integration with computer systems.
Automatically generated - may contain errors
Lab products found in correlation
Bx51 microscope, by Olympus (3 mentions)
Rm2125rt, by Leica (1 mentions)
Szx10 research high class stereo microscope, by Olympus (1 mentions)
Xc30 microscope, by Olympus (1 mentions)
Corresponding assay kits, by Abcam (1 mentions)
Stream basic software, by Olympus (1 mentions)
Bx41 microscope, by Olympus (1 mentions)
Stream basic, by Olympus (1 mentions)
6 protocols using uc30 digital camera
1
Histological Analysis of Mandibular Lesions
2
Morphological Identification of Larval Trematodes
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Larval stages were preliminarily identified under an Olympus BX51 microscope (Tokyo, Japan) using morphological descriptions of Našincová (1992) and Bykhovskaya-Pavlovskaya & Kulakova (1971) and other relevant publications (e.g. Heinemann, 1937; Zdun, 1961; Našincová & Scholz, 1994; Kudlai et al., 2015) . Preliminary identification was made to the family or genus level. Morphology of cercariae was studied on live and fixed specimens. Series of photomicrographs were taken for collected isolates with an Olympus UC30 digital camera (Tokyo, Japan) for measurements and further identification. Measurements were taken from the digital images using cellSens 1.16 Life Science image software (https://www.olympus-lifescience. com/en/software/cellsens). Measurements are in micrometres (μm) and are presented as a range, followed by a mean in parentheses.
3
Microplastic Identification Using Microscopy
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The filters were observed under an Olympus SZX10 Research High-Class Stereo microscope (Olympus Corporation, Japan), and photographed with an Olympus UC30 digital camera. A visual assessment was conducted to identify particles according to the physical characteristics. MPs were classified as fibers, film, fragments or spheres using the descriptions from Tagg et al. (2015) . A number of commonly detected particles were selected and verified with a micro-FT-IR, iNicolet, Thermofisher Scientific) cooled with liquid nitrogen (Tagg et al. 2015) . Analysis was conducted in transmittance mode with MPs mounted on a diamond compression cell. Spectra were acquired and matched using a series of polymer library databases (Hummel), a hit index of at least 70% was considered acceptable.
4
Histological Analysis of Brain Samples
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Brain samples were excised and post-fixed for 48 h at room temperature in 4% paraformaldehyde. Specimens were fixed in 5% buffered formalin, dried, and cleaned before being embedded in paraffin. Serial sections with a five-micrometer thickness were placed on slides coated with poly-L-lysine, deparaffinized in xylene, and rehydrated in ethanol. The serial sections were then stained with hematoxylin and eosin (H&E) in accordance with the standard procedure for histological analysis of sections from various groups [56, 57] (link). Representative photographs were captured at different magnifications of each experimental group with an Olympus UC30 digital camera and an Olympus XC30 microscope (Germany).
5
Liver Protein and Oxidative Stress Analysis
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Extraction of total protein from liver tissue was done by homogenization with a phosphate buffer solution (at 10 mM, pH 7.4). The obtained protein extracts were analyzed for total protein content, catalase (CAT), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity, and lipid peroxidation. The activity of CAT, XOD and GPx was determined using the corresponding assay kits (BioVision, USA), according to the manufacturer’s specifications. The concentration of malonyl dialdehyde (MDA) was determined using reaction with thiobarbituric acid (TBA). The results were measured by a METERTECH Spectrophotometer SP-830 Plus.
For histopathological examination, the tissue fragments were first placed in 10% formalin solution for 24 hours, processed for paraffin embedding and cut to a thickness of 5–6 μm. The slides were than stained with the standard histological hematoxylin-eosin stain. The microscopic image was obtained using the Olympus BX 41 microscope. Image acquisition was performed using an Olympus UC30 digital camera and OLYMPUS Stream Basic software. Histopathological examination aimed to identify the main degenerative, necrotic and inflammatory processes present in the liver, at different stages of the pathological process and at different extract doses.
For histopathological examination, the tissue fragments were first placed in 10% formalin solution for 24 hours, processed for paraffin embedding and cut to a thickness of 5–6 μm. The slides were than stained with the standard histological hematoxylin-eosin stain. The microscopic image was obtained using the Olympus BX 41 microscope. Image acquisition was performed using an Olympus UC30 digital camera and OLYMPUS Stream Basic software. Histopathological examination aimed to identify the main degenerative, necrotic and inflammatory processes present in the liver, at different stages of the pathological process and at different extract doses.
6
Histological Examination of Hepatic Samples
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For the histological study, the hepatic samples were fixed in 10% buffered neutral formalin, embedded in paraffin, while sections were made at 4 micrometers and the slides were stained with hematoxylin and eosin (H&E) dye. Then the slides were examined under an Olympus BX 51 microscope. The images were taken with an Olympus UC 30 digital camera and processed by a special image acquisition and processing program, i.e. Olympus Stream Basic.
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