Dylight conjugated goat anti hrp antibody

Larvae were dissected and stained as described previously[27 (link), 67 (link)]. Following primary antibodies were used: anti-BRP (1:250)[16 (link)], anti-Tubulin (E7) (1:100) (obtained from the Developmental Studies Hybridoma Bank), anti-GFP (1:500)[41 (link)] (obtained from abcam), anti-DVGLUT (1:10,000)[19 (link)](gift from Aaron Diantonio, Washington University Medical School), anti-Liprin-α (1:500)[68 (link)](gift from Stephan Sigrist, Free University Berlin), anti-DAB [24 (link)](gift from Richard Ordway, Pennsylvania State University), and anti-dTau (1:1000)[34 (link), 35 (link)](gift from Doris Kretzschmar, Oregon Health and Science University and Daniel St. Johnston, University of Cambridge). Dylight conjugated goat anti-HRP antibody (1:1,000), Goat Cy3-, and Alexa 488 conjugated secondary antibodies against mouse, rabbit, and chicken IgG (1:1000) were obtained from Jackson ImmunoResearch, West Grove, PA.

Larvae were dissected and stained as previously described^{54 (link)}. Briefly, the larvae were dissected in cold 1X PBS solution followed by fixation in Bouin’s fixative for 5 minutes. Larvae were washed 3X with PBS-Triton (0.1% solution) and blocked using 5% NGS solution in PBS. Following primary antibodies were used: anti-BRP (1:250, DSHB, Iowa city, IA)^{55 (link)}, anti-Futsch (1:100, DSHB, Iowa City, IA)^{22 (link)}, anti-DVGLUT (1:10,000, gift from Aaron DiAntonio, Washington University in St. Louis, MO)^{56 (link)} and anti-Par-1 (1:1,000, gift from Bingwei Lu, Stanford School of Medicine, CA)^{17 (link)}. Dylight conjugated goat anti-HRP antibody (1:1,000), Goat Cy3-, and Alexa 488 conjugated secondary antibodies against mouse and rabbit IgG (1:1000) were obtained from Jackson ImmunoResearch, West Grove, PA.