Prior to their analysis, the samples (1 mL) were processed and aliquoted as described previously [28 (link)]. Every assay was run in duplicate according to the manufacturer's instructions and standard curves were performed for each analyte.
Raybio human egf elisa kit
The RayBio Human EGF ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human epidermal growth factor (EGF) in cell culture supernatants, serum, and plasma samples.
3 protocols using raybio human egf elisa kit
Milk Immune Factors Quantification
Prior to their analysis, the samples (1 mL) were processed and aliquoted as described previously [28 (link)]. Every assay was run in duplicate according to the manufacturer's instructions and standard curves were performed for each analyte.
Biomarker Profiling of Human Milk
The inter-assay coefficients of variation were below manufacturers' instructions for all the immune markers, and the lower limit of quantification (LLOQ) for every analyte in human milk were: 0.21 μg/L for IgA, 0.78 μg/L for IgM, 2.19 μg/L for IgG, 1.57 ng/L for TGF-β2, 0.03 ng/L for EGF, 23.10 ng/L for adiponectin, 7.40 ng/L for ghrelin, and 11.45 ng/L for leptin.
Cytokine Profiling of HUVEC Response to Borrelia
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